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Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus
The C2H2-type zinc finger proteins (C2H2-ZFPs) regulate various developmental processes and abiotic stress responses in eukaryotes. Yet, a comprehensive analysis of these transcription factors which could be used to find candidate genes related to the control the development and abiotic stress toler...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742544/ https://www.ncbi.nlm.nih.gov/pubmed/35036086 http://dx.doi.org/10.7717/peerj.12654 |
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author | Ding, Qiangqiang Zhao, Hongyuan Zhu, Peilei Jiang, Xiangting Nie, Fan Li, Guoqing |
author_facet | Ding, Qiangqiang Zhao, Hongyuan Zhu, Peilei Jiang, Xiangting Nie, Fan Li, Guoqing |
author_sort | Ding, Qiangqiang |
collection | PubMed |
description | The C2H2-type zinc finger proteins (C2H2-ZFPs) regulate various developmental processes and abiotic stress responses in eukaryotes. Yet, a comprehensive analysis of these transcription factors which could be used to find candidate genes related to the control the development and abiotic stress tolerance has not been performed in Pleurotus ostreatus. To fill this knowledge gap, 18 C2H2-ZFs were identified in the P. ostreatus genome. Phylogenetic analysis indicated that these proteins have dissimilar amino acid sequences. In addition, these proteins had variable protein characteristics, gene intron-exon structures, and motif compositions. The expression patterns of PoC2H2-ZFs in mycelia, primordia, and young and mature fruiting bodies were investigated using qRT-PCR. The expression of some PoC2H2-ZFs is regulated by auxin and cytokinin. Moreover, members of PoC2H2-ZFs expression levels are changed dramatically under heat and cold stress, suggesting that these genes may participate in abiotic stress responses. These findings could be used to study the role of P. ostreatus-derived C2H2-ZFs in development and stress tolerance. |
format | Online Article Text |
id | pubmed-8742544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87425442022-01-14 Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus Ding, Qiangqiang Zhao, Hongyuan Zhu, Peilei Jiang, Xiangting Nie, Fan Li, Guoqing PeerJ Agricultural Science The C2H2-type zinc finger proteins (C2H2-ZFPs) regulate various developmental processes and abiotic stress responses in eukaryotes. Yet, a comprehensive analysis of these transcription factors which could be used to find candidate genes related to the control the development and abiotic stress tolerance has not been performed in Pleurotus ostreatus. To fill this knowledge gap, 18 C2H2-ZFs were identified in the P. ostreatus genome. Phylogenetic analysis indicated that these proteins have dissimilar amino acid sequences. In addition, these proteins had variable protein characteristics, gene intron-exon structures, and motif compositions. The expression patterns of PoC2H2-ZFs in mycelia, primordia, and young and mature fruiting bodies were investigated using qRT-PCR. The expression of some PoC2H2-ZFs is regulated by auxin and cytokinin. Moreover, members of PoC2H2-ZFs expression levels are changed dramatically under heat and cold stress, suggesting that these genes may participate in abiotic stress responses. These findings could be used to study the role of P. ostreatus-derived C2H2-ZFs in development and stress tolerance. PeerJ Inc. 2022-01-05 /pmc/articles/PMC8742544/ /pubmed/35036086 http://dx.doi.org/10.7717/peerj.12654 Text en ©2022 Ding et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Ding, Qiangqiang Zhao, Hongyuan Zhu, Peilei Jiang, Xiangting Nie, Fan Li, Guoqing Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
title | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
title_full | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
title_fullStr | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
title_full_unstemmed | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
title_short | Genome-wide identification and expression analyses of C2H2 zinc finger transcription factors in Pleurotus ostreatus |
title_sort | genome-wide identification and expression analyses of c2h2 zinc finger transcription factors in pleurotus ostreatus |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742544/ https://www.ncbi.nlm.nih.gov/pubmed/35036086 http://dx.doi.org/10.7717/peerj.12654 |
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