Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant

A Gram-stain-negative, yellow-pigmented bacterium, designated as L7(T), was isolated from seeds of Alhagi sparsifolia Shap., a leguminous plant that grows in northwest PR China. Strain L7(T) was found to be non-flagellated, non-spore forming rods which can grow at 10–37 °C, pH 6.0–8.5 and in 0–3 % (v/w) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain L7(T) belongs to the genus Chryseobacterium with sequence similarities to Chryseobacterium vietnamense GIMN1.005(T) (98.1%), C. bernardetii NCCTC13530(T) (98.0%), C. vrystaatense LMG 22846(T) (97.9%), C. nakagawai NCTC13529(T) (97.7%), C. shigense DSM 17126(T) (97.6%) and C. rhizosphaerae RSB3-1(T) (97.5%). The average nucleotide identity of strain L7(T) to 31 reference strains were 78.6–85.6 %, lower than the species delineation threshold of 95 %. MK-6 was the only respiratory quinone of L7(T) and major fatty acids were iso-C(15 : 0), iso-C(17 : 0) 3-OH, C(16 : 1) ω7c and/or C(16 : 1) ω6c, isoC(17 : 1) ω9c and/or C(16 : 0) 10-methyl. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, two unidentified aminolipids, three unidentified glycolipids and two unidentified lipids. The G+C content of the genome was 38.58 mol%. On the basis of polyphasic taxonomy analyses in this study, strain L7(T) is considered to represent a novel species in the genus Chryseobacterium , for which the name Chryseobacterium endalhagicum sp. nov. is proposed. The type strain is L7(T) (=MCCC 1K05687(T)=JCM 34506(T))