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Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant

A Gram-stain-negative, yellow-pigmented bacterium, designated as L7(T), was isolated from seeds of Alhagi sparsifolia Shap., a leguminous plant that grows in northwest PR China. Strain L7(T) was found to be non-flagellated, non-spore forming rods which can grow at 10–37 °C, pH 6.0–8.5 and in 0–3 % (...

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Autores principales: Zhang, Xiaobo, Guo, Xingyan, Kahaer, Mayina, Tian, Tingting, Sun, Yuping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742555/
https://www.ncbi.nlm.nih.gov/pubmed/34739366
http://dx.doi.org/10.1099/ijsem.0.005077
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author Zhang, Xiaobo
Guo, Xingyan
Kahaer, Mayina
Tian, Tingting
Sun, Yuping
author_facet Zhang, Xiaobo
Guo, Xingyan
Kahaer, Mayina
Tian, Tingting
Sun, Yuping
author_sort Zhang, Xiaobo
collection PubMed
description A Gram-stain-negative, yellow-pigmented bacterium, designated as L7(T), was isolated from seeds of Alhagi sparsifolia Shap., a leguminous plant that grows in northwest PR China. Strain L7(T) was found to be non-flagellated, non-spore forming rods which can grow at 10–37 °C, pH 6.0–8.5 and in 0–3 % (v/w) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain L7(T) belongs to the genus Chryseobacterium with sequence similarities to Chryseobacterium vietnamense GIMN1.005(T) (98.1%), C. bernardetii NCCTC13530(T) (98.0%), C. vrystaatense LMG 22846(T) (97.9%), C. nakagawai NCTC13529(T) (97.7%), C. shigense DSM 17126(T) (97.6%) and C. rhizosphaerae RSB3-1(T) (97.5%). The average nucleotide identity of strain L7(T) to 31 reference strains were 78.6–85.6 %, lower than the species delineation threshold of 95 %. MK-6 was the only respiratory quinone of L7(T) and major fatty acids were iso-C(15 : 0), iso-C(17 : 0) 3-OH, C(16 : 1)  ω7c and/or C(16 : 1)  ω6c, isoC(17 : 1)  ω9c and/or C(16 : 0) 10-methyl. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, two unidentified aminolipids, three unidentified glycolipids and two unidentified lipids. The G+C content of the genome was 38.58 mol%. On the basis of polyphasic taxonomy analyses in this study, strain L7(T) is considered to represent a novel species in the genus Chryseobacterium , for which the name Chryseobacterium endalhagicum sp. nov. is proposed. The type strain is L7(T) (=MCCC 1K05687(T)=JCM 34506(T))
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spelling pubmed-87425552022-01-10 Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant Zhang, Xiaobo Guo, Xingyan Kahaer, Mayina Tian, Tingting Sun, Yuping Int J Syst Evol Microbiol New Taxa A Gram-stain-negative, yellow-pigmented bacterium, designated as L7(T), was isolated from seeds of Alhagi sparsifolia Shap., a leguminous plant that grows in northwest PR China. Strain L7(T) was found to be non-flagellated, non-spore forming rods which can grow at 10–37 °C, pH 6.0–8.5 and in 0–3 % (v/w) NaCl concentration. The 16S rRNA gene sequence analysis showed that strain L7(T) belongs to the genus Chryseobacterium with sequence similarities to Chryseobacterium vietnamense GIMN1.005(T) (98.1%), C. bernardetii NCCTC13530(T) (98.0%), C. vrystaatense LMG 22846(T) (97.9%), C. nakagawai NCTC13529(T) (97.7%), C. shigense DSM 17126(T) (97.6%) and C. rhizosphaerae RSB3-1(T) (97.5%). The average nucleotide identity of strain L7(T) to 31 reference strains were 78.6–85.6 %, lower than the species delineation threshold of 95 %. MK-6 was the only respiratory quinone of L7(T) and major fatty acids were iso-C(15 : 0), iso-C(17 : 0) 3-OH, C(16 : 1)  ω7c and/or C(16 : 1)  ω6c, isoC(17 : 1)  ω9c and/or C(16 : 0) 10-methyl. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, two unidentified aminolipids, three unidentified glycolipids and two unidentified lipids. The G+C content of the genome was 38.58 mol%. On the basis of polyphasic taxonomy analyses in this study, strain L7(T) is considered to represent a novel species in the genus Chryseobacterium , for which the name Chryseobacterium endalhagicum sp. nov. is proposed. The type strain is L7(T) (=MCCC 1K05687(T)=JCM 34506(T)) Microbiology Society 2021-11-05 /pmc/articles/PMC8742555/ /pubmed/34739366 http://dx.doi.org/10.1099/ijsem.0.005077 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle New Taxa
Zhang, Xiaobo
Guo, Xingyan
Kahaer, Mayina
Tian, Tingting
Sun, Yuping
Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant
title Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant
title_full Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant
title_fullStr Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant
title_full_unstemmed Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant
title_short Chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant
title_sort chryseobacterium endalhagicum sp. nov., isolated from seed of leguminous plant
topic New Taxa
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742555/
https://www.ncbi.nlm.nih.gov/pubmed/34739366
http://dx.doi.org/10.1099/ijsem.0.005077
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