Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells

Epstein–Barr virus (EBV) is present in a state of latency in infected memory B-cells and EBV-associated lymphoid and epithelial cancers. Cell stimulation or differentiation of infected B-cells and epithelial cells induces reactivation to the lytic replication cycle. In each cell type, the EBV transc...

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Autores principales: Godfrey, Anja, Osborn, Kay, Sinclair, Alison J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742585/
https://www.ncbi.nlm.nih.gov/pubmed/35018326
http://dx.doi.org/10.1099/acmi.0.000282
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author Godfrey, Anja
Osborn, Kay
Sinclair, Alison J.
author_facet Godfrey, Anja
Osborn, Kay
Sinclair, Alison J.
author_sort Godfrey, Anja
collection PubMed
description Epstein–Barr virus (EBV) is present in a state of latency in infected memory B-cells and EBV-associated lymphoid and epithelial cancers. Cell stimulation or differentiation of infected B-cells and epithelial cells induces reactivation to the lytic replication cycle. In each cell type, the EBV transcription and replication factor Zta (BZLF1, EB1) plays a role in mediating the lytic cycle of EBV. Zta is a transcription factor that interacts directly with Zta response elements (ZREs) within viral and cellular genomes. Here we undertake chromatin-precipitation coupled to DNA-sequencing (ChIP-Seq) of Zta-associated DNA from cancer-derived epithelial cells. The analysis identified over 14 000 Zta-binding sites in the cellular genome. We assessed the impact of lytic cycle reactivation on changes in gene expression for a panel of Zta-associated cellular genes. Finally, we compared the Zta-binding sites identified in this study with those previously identified in B-cells and reveal substantial conservation in genes associated with Zta-binding sites.
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spelling pubmed-87425852022-01-10 Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells Godfrey, Anja Osborn, Kay Sinclair, Alison J. Access Microbiol Research Articles Epstein–Barr virus (EBV) is present in a state of latency in infected memory B-cells and EBV-associated lymphoid and epithelial cancers. Cell stimulation or differentiation of infected B-cells and epithelial cells induces reactivation to the lytic replication cycle. In each cell type, the EBV transcription and replication factor Zta (BZLF1, EB1) plays a role in mediating the lytic cycle of EBV. Zta is a transcription factor that interacts directly with Zta response elements (ZREs) within viral and cellular genomes. Here we undertake chromatin-precipitation coupled to DNA-sequencing (ChIP-Seq) of Zta-associated DNA from cancer-derived epithelial cells. The analysis identified over 14 000 Zta-binding sites in the cellular genome. We assessed the impact of lytic cycle reactivation on changes in gene expression for a panel of Zta-associated cellular genes. Finally, we compared the Zta-binding sites identified in this study with those previously identified in B-cells and reveal substantial conservation in genes associated with Zta-binding sites. Microbiology Society 2021-11-26 /pmc/articles/PMC8742585/ /pubmed/35018326 http://dx.doi.org/10.1099/acmi.0.000282 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle Research Articles
Godfrey, Anja
Osborn, Kay
Sinclair, Alison J.
Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells
title Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells
title_full Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells
title_fullStr Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells
title_full_unstemmed Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells
title_short Interaction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells
title_sort interaction sites of the epstein–barr virus zta transcription factor with the host genome in epithelial cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742585/
https://www.ncbi.nlm.nih.gov/pubmed/35018326
http://dx.doi.org/10.1099/acmi.0.000282
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