Cargando…
Expression and Clinical Value of miR-185 and miR-424 in Patients with Acute Ischemic Stroke
BACKGROUND: To investigate the expression of serum miR-185 and miR-424 in patients with acute ischemic stroke (AIS) and their predictive value. A total of 142 patients with AIS and 50 healthy controls were enrolled. METHODS: According to the modified Rankin scale (mRS) score, AIS patients were divid...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742602/ https://www.ncbi.nlm.nih.gov/pubmed/35018114 http://dx.doi.org/10.2147/IJGM.S340586 |
_version_ | 1784629752077746176 |
---|---|
author | Guo, Chunxuan Yao, Yanping Li, Qi Gao, Yuanjie Cao, Hongyan |
author_facet | Guo, Chunxuan Yao, Yanping Li, Qi Gao, Yuanjie Cao, Hongyan |
author_sort | Guo, Chunxuan |
collection | PubMed |
description | BACKGROUND: To investigate the expression of serum miR-185 and miR-424 in patients with acute ischemic stroke (AIS) and their predictive value. A total of 142 patients with AIS and 50 healthy controls were enrolled. METHODS: According to the modified Rankin scale (mRS) score, AIS patients were divided into the good prognosis group and the poor prognosis group. Based on the National Institutes of Health Stroke Scale (NIHSS) score, AIS patients were divided into the mild group, the moderate group, and the severe group. RT-qPCR was used to determine the expression. ROC curve and Pearson correlation analysis were adopted to predict poor prognosis and analyze the correlation between the expression and NIHSS, mRS score. RESULTS: Compared with the control group, the expression of miR-185 and miR-424 in the AIS group was significantly higher (P<0.01). Similarly, significantly higher expressions could be found in the poor prognosis group and the severe group (P<0.01). The ROC curve revealed that the optimal cut-off values were 2.14 and 4.08, respectively. The area under the ROC curve (0.928, 95% CI: 0.870–0.993) was the largest, with sensitivity and specificity of 92.0% and 85.7%. Pearson correlation analysis showed that their expression was positively correlated with NIHSS score and mRS score in AIS patients (r=0.735, 0.802, 0.796, 0.873, P<0.01). CONCLUSION: There are two factors related to the up-regulated expression of serum miR-185 and miR-424, one is the severity degree of neurological impairment of patients with AIS and the other is their prognosis. These two combined indicators can contribute to predicting the prognosis of AIS patients. |
format | Online Article Text |
id | pubmed-8742602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-87426022022-01-10 Expression and Clinical Value of miR-185 and miR-424 in Patients with Acute Ischemic Stroke Guo, Chunxuan Yao, Yanping Li, Qi Gao, Yuanjie Cao, Hongyan Int J Gen Med Original Research BACKGROUND: To investigate the expression of serum miR-185 and miR-424 in patients with acute ischemic stroke (AIS) and their predictive value. A total of 142 patients with AIS and 50 healthy controls were enrolled. METHODS: According to the modified Rankin scale (mRS) score, AIS patients were divided into the good prognosis group and the poor prognosis group. Based on the National Institutes of Health Stroke Scale (NIHSS) score, AIS patients were divided into the mild group, the moderate group, and the severe group. RT-qPCR was used to determine the expression. ROC curve and Pearson correlation analysis were adopted to predict poor prognosis and analyze the correlation between the expression and NIHSS, mRS score. RESULTS: Compared with the control group, the expression of miR-185 and miR-424 in the AIS group was significantly higher (P<0.01). Similarly, significantly higher expressions could be found in the poor prognosis group and the severe group (P<0.01). The ROC curve revealed that the optimal cut-off values were 2.14 and 4.08, respectively. The area under the ROC curve (0.928, 95% CI: 0.870–0.993) was the largest, with sensitivity and specificity of 92.0% and 85.7%. Pearson correlation analysis showed that their expression was positively correlated with NIHSS score and mRS score in AIS patients (r=0.735, 0.802, 0.796, 0.873, P<0.01). CONCLUSION: There are two factors related to the up-regulated expression of serum miR-185 and miR-424, one is the severity degree of neurological impairment of patients with AIS and the other is their prognosis. These two combined indicators can contribute to predicting the prognosis of AIS patients. Dove 2022-01-04 /pmc/articles/PMC8742602/ /pubmed/35018114 http://dx.doi.org/10.2147/IJGM.S340586 Text en © 2022 Guo et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Guo, Chunxuan Yao, Yanping Li, Qi Gao, Yuanjie Cao, Hongyan Expression and Clinical Value of miR-185 and miR-424 in Patients with Acute Ischemic Stroke |
title | Expression and Clinical Value of miR-185 and miR-424 in Patients with Acute Ischemic Stroke |
title_full | Expression and Clinical Value of miR-185 and miR-424 in Patients with Acute Ischemic Stroke |
title_fullStr | Expression and Clinical Value of miR-185 and miR-424 in Patients with Acute Ischemic Stroke |
title_full_unstemmed | Expression and Clinical Value of miR-185 and miR-424 in Patients with Acute Ischemic Stroke |
title_short | Expression and Clinical Value of miR-185 and miR-424 in Patients with Acute Ischemic Stroke |
title_sort | expression and clinical value of mir-185 and mir-424 in patients with acute ischemic stroke |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742602/ https://www.ncbi.nlm.nih.gov/pubmed/35018114 http://dx.doi.org/10.2147/IJGM.S340586 |
work_keys_str_mv | AT guochunxuan expressionandclinicalvalueofmir185andmir424inpatientswithacuteischemicstroke AT yaoyanping expressionandclinicalvalueofmir185andmir424inpatientswithacuteischemicstroke AT liqi expressionandclinicalvalueofmir185andmir424inpatientswithacuteischemicstroke AT gaoyuanjie expressionandclinicalvalueofmir185andmir424inpatientswithacuteischemicstroke AT caohongyan expressionandclinicalvalueofmir185andmir424inpatientswithacuteischemicstroke |