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MicroRNAs affect GPCR and Ion channel genes needed for influenza replication

Influenza virus causes seasonal epidemics and sporadic pandemics resulting in morbidity, mortality, and economic losses worldwide. Understanding how to regulate influenza virus replication is important for developing vaccine and therapeutic strategies. Identifying microRNAs (miRs) that affect host g...

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Autores principales: Orr-Burks, Nichole, Murray, Jackelyn, Todd, Kyle V., Bakre, Abhijeet, Tripp, Ralph A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742985/
https://www.ncbi.nlm.nih.gov/pubmed/34787540
http://dx.doi.org/10.1099/jgv.0.001691
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author Orr-Burks, Nichole
Murray, Jackelyn
Todd, Kyle V.
Bakre, Abhijeet
Tripp, Ralph A.
author_facet Orr-Burks, Nichole
Murray, Jackelyn
Todd, Kyle V.
Bakre, Abhijeet
Tripp, Ralph A.
author_sort Orr-Burks, Nichole
collection PubMed
description Influenza virus causes seasonal epidemics and sporadic pandemics resulting in morbidity, mortality, and economic losses worldwide. Understanding how to regulate influenza virus replication is important for developing vaccine and therapeutic strategies. Identifying microRNAs (miRs) that affect host genes used by influenza virus for replication can support an antiviral strategy. In this study, G-protein coupled receptor (GPCR) and ion channel (IC) host genes in human alveolar epithelial (A549) cells used by influenza virus for replication (Orr-Burks et al., 2021) were examined as miR target genes following A/CA/04/09- or B/Yamagata/16/1988 replication. Thirty-three miRs were predicted to target GPCR or IC genes and their miR mimics were evaluated for their ability to decrease influenza virus replication. Paired miR inhibitors were used as an ancillary measure to confirm or not the antiviral effects of a miR mimic. Fifteen miRs lowered influenza virus replication and four miRs were found to reduce replication irrespective of virus strain and type differences. These findings provide evidence for novel miR disease intervention strategies for influenza viruses.
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spelling pubmed-87429852022-01-10 MicroRNAs affect GPCR and Ion channel genes needed for influenza replication Orr-Burks, Nichole Murray, Jackelyn Todd, Kyle V. Bakre, Abhijeet Tripp, Ralph A. J Gen Virol Animal Influenza virus causes seasonal epidemics and sporadic pandemics resulting in morbidity, mortality, and economic losses worldwide. Understanding how to regulate influenza virus replication is important for developing vaccine and therapeutic strategies. Identifying microRNAs (miRs) that affect host genes used by influenza virus for replication can support an antiviral strategy. In this study, G-protein coupled receptor (GPCR) and ion channel (IC) host genes in human alveolar epithelial (A549) cells used by influenza virus for replication (Orr-Burks et al., 2021) were examined as miR target genes following A/CA/04/09- or B/Yamagata/16/1988 replication. Thirty-three miRs were predicted to target GPCR or IC genes and their miR mimics were evaluated for their ability to decrease influenza virus replication. Paired miR inhibitors were used as an ancillary measure to confirm or not the antiviral effects of a miR mimic. Fifteen miRs lowered influenza virus replication and four miRs were found to reduce replication irrespective of virus strain and type differences. These findings provide evidence for novel miR disease intervention strategies for influenza viruses. Microbiology Society 2021-11-17 /pmc/articles/PMC8742985/ /pubmed/34787540 http://dx.doi.org/10.1099/jgv.0.001691 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
spellingShingle Animal
Orr-Burks, Nichole
Murray, Jackelyn
Todd, Kyle V.
Bakre, Abhijeet
Tripp, Ralph A.
MicroRNAs affect GPCR and Ion channel genes needed for influenza replication
title MicroRNAs affect GPCR and Ion channel genes needed for influenza replication
title_full MicroRNAs affect GPCR and Ion channel genes needed for influenza replication
title_fullStr MicroRNAs affect GPCR and Ion channel genes needed for influenza replication
title_full_unstemmed MicroRNAs affect GPCR and Ion channel genes needed for influenza replication
title_short MicroRNAs affect GPCR and Ion channel genes needed for influenza replication
title_sort micrornas affect gpcr and ion channel genes needed for influenza replication
topic Animal
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8742985/
https://www.ncbi.nlm.nih.gov/pubmed/34787540
http://dx.doi.org/10.1099/jgv.0.001691
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