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Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production
Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications. Different strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles. Optimizing t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743079/ http://dx.doi.org/10.1007/s43393-021-00069-1 |
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author | Udayan, Aswathy Sreekumar, Nidhin Arumugam, Muthu |
author_facet | Udayan, Aswathy Sreekumar, Nidhin Arumugam, Muthu |
author_sort | Udayan, Aswathy |
collection | PubMed |
description | Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications. Different strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles. Optimizing the composition of media for the cultivation of microalgae to induce biomass production and high-value metabolite accumulation has been considered as an important factor for sustainable product development. In this study, the effect of plant growth regulators together with basal microalgal cultivation medium on biomass, total lipid, and EPA production was studied using the Plackett–Burman model and Response surface methodology. The traditional one-factor-at-a-time optimization approach is laborious, time-consuming, and requires more experiments which makes the process and analysis more difficult. The Designed PB model was found to be significant for biomass (396 mg/L), lipid (254 mg/L), and EPA (5.6%) production with a P value < 0.05. The major objective of this study is to formulate a medium for EPA production without compromising the growth properties. Further, we had formulated a new media using RSM to achieve the goal and the significant variables selected were NaNO(3), NaH(2)PO(4,) and IAA and was found to be significant with 16.72% EPA production with a biomass production of 893 mg/L with a P value < 0.05. The formulated medium can be used in large-scale cultivation systems which can enhance biomass production as well as the omega 3 fatty acid production in marine microalgae Nannochloropsis oceanica. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43393-021-00069-1. |
format | Online Article Text |
id | pubmed-8743079 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-87430792022-01-10 Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production Udayan, Aswathy Sreekumar, Nidhin Arumugam, Muthu Syst Microbiol and Biomanuf Original Article Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications. Different strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles. Optimizing the composition of media for the cultivation of microalgae to induce biomass production and high-value metabolite accumulation has been considered as an important factor for sustainable product development. In this study, the effect of plant growth regulators together with basal microalgal cultivation medium on biomass, total lipid, and EPA production was studied using the Plackett–Burman model and Response surface methodology. The traditional one-factor-at-a-time optimization approach is laborious, time-consuming, and requires more experiments which makes the process and analysis more difficult. The Designed PB model was found to be significant for biomass (396 mg/L), lipid (254 mg/L), and EPA (5.6%) production with a P value < 0.05. The major objective of this study is to formulate a medium for EPA production without compromising the growth properties. Further, we had formulated a new media using RSM to achieve the goal and the significant variables selected were NaNO(3), NaH(2)PO(4,) and IAA and was found to be significant with 16.72% EPA production with a biomass production of 893 mg/L with a P value < 0.05. The formulated medium can be used in large-scale cultivation systems which can enhance biomass production as well as the omega 3 fatty acid production in marine microalgae Nannochloropsis oceanica. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43393-021-00069-1. Springer Singapore 2022-01-10 2022 /pmc/articles/PMC8743079/ http://dx.doi.org/10.1007/s43393-021-00069-1 Text en © Jiangnan University 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Article Udayan, Aswathy Sreekumar, Nidhin Arumugam, Muthu Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production |
title | Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production |
title_full | Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production |
title_fullStr | Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production |
title_full_unstemmed | Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production |
title_short | Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production |
title_sort | statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743079/ http://dx.doi.org/10.1007/s43393-021-00069-1 |
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