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Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production

Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications. Different strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles. Optimizing t...

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Autores principales: Udayan, Aswathy, Sreekumar, Nidhin, Arumugam, Muthu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743079/
http://dx.doi.org/10.1007/s43393-021-00069-1
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author Udayan, Aswathy
Sreekumar, Nidhin
Arumugam, Muthu
author_facet Udayan, Aswathy
Sreekumar, Nidhin
Arumugam, Muthu
author_sort Udayan, Aswathy
collection PubMed
description Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications. Different strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles. Optimizing the composition of media for the cultivation of microalgae to induce biomass production and high-value metabolite accumulation has been considered as an important factor for sustainable product development. In this study, the effect of plant growth regulators together with basal microalgal cultivation medium on biomass, total lipid, and EPA production was studied using the Plackett–Burman model and Response surface methodology. The traditional one-factor-at-a-time optimization approach is laborious, time-consuming, and requires more experiments which makes the process and analysis more difficult. The Designed PB model was found to be significant for biomass (396 mg/L), lipid (254 mg/L), and EPA (5.6%) production with a P value < 0.05. The major objective of this study is to formulate a medium for EPA production without compromising the growth properties. Further, we had formulated a new media using RSM to achieve the goal and the significant variables selected were NaNO(3), NaH(2)PO(4,) and IAA and was found to be significant with 16.72% EPA production with a biomass production of 893 mg/L with a P value < 0.05. The formulated medium can be used in large-scale cultivation systems which can enhance biomass production as well as the omega 3 fatty acid production in marine microalgae Nannochloropsis oceanica. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43393-021-00069-1.
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spelling pubmed-87430792022-01-10 Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production Udayan, Aswathy Sreekumar, Nidhin Arumugam, Muthu Syst Microbiol and Biomanuf Original Article Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications. Different strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles. Optimizing the composition of media for the cultivation of microalgae to induce biomass production and high-value metabolite accumulation has been considered as an important factor for sustainable product development. In this study, the effect of plant growth regulators together with basal microalgal cultivation medium on biomass, total lipid, and EPA production was studied using the Plackett–Burman model and Response surface methodology. The traditional one-factor-at-a-time optimization approach is laborious, time-consuming, and requires more experiments which makes the process and analysis more difficult. The Designed PB model was found to be significant for biomass (396 mg/L), lipid (254 mg/L), and EPA (5.6%) production with a P value < 0.05. The major objective of this study is to formulate a medium for EPA production without compromising the growth properties. Further, we had formulated a new media using RSM to achieve the goal and the significant variables selected were NaNO(3), NaH(2)PO(4,) and IAA and was found to be significant with 16.72% EPA production with a biomass production of 893 mg/L with a P value < 0.05. The formulated medium can be used in large-scale cultivation systems which can enhance biomass production as well as the omega 3 fatty acid production in marine microalgae Nannochloropsis oceanica. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s43393-021-00069-1. Springer Singapore 2022-01-10 2022 /pmc/articles/PMC8743079/ http://dx.doi.org/10.1007/s43393-021-00069-1 Text en © Jiangnan University 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Original Article
Udayan, Aswathy
Sreekumar, Nidhin
Arumugam, Muthu
Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production
title Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production
title_full Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production
title_fullStr Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production
title_full_unstemmed Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production
title_short Statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production
title_sort statistical optimization and formulation of microalga cultivation medium for improved omega 3 fatty acid production
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743079/
http://dx.doi.org/10.1007/s43393-021-00069-1
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