Cargando…

Establishment and evaluation of a 30-minute detection method for SARS-CoV-2 nucleic acid using a novel ultra-fast real-time PCR instrument

BACKGROUND: The coronavirus disease 2019 (COVID-19) pandemic is still raging worldwide. Efficient, fast and low-cost severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid detection methods are urgently needed. METHODS: A rapid PCR temperature change mode was explored by moving th...

Descripción completa

Detalles Bibliográficos
Autores principales:	Liu, Wenkuan, Zhou, Zhichao, Zhang, Lu, Li, Lei, Wang, Lin, Song, Linxiu, Qiu, Shuyan, Zhang, Li, Xu, Duo, Tian, Xingui, Li, Xiao, Yang, Yujie, Liang, Jiaxin, Liu, Yong, Li, Xiaobo, Zhou, Rong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	AME Publishing Company 2021
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743416/ https://www.ncbi.nlm.nih.gov/pubmed/35070371 http://dx.doi.org/10.21037/jtd-21-1288

Ejemplares similares

Abnormal liver function in children hospitalized with acute respiratory infection of adenoviruses: a retrospective study
por: Tian, Xingui, et al.
Publicado: (2023)

Analysis of severe human adenovirus infection outbreak in Guangdong Province, southern China in 2019
por: Liu, Wenkuan, et al.
Publicado: (2022)

Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody
por: Tian, Xingui, et al.
Publicado: (2021)

Identification of conserved linear epitopes in the SARS-CoV-2 receptor-binding region using monoclonal antibodies
por: Yang, Yujie, et al.
Publicado: (2023)

A NOVEL ULTRAFAST REAL-TIME PCR INSTRUMENT FOR RAPID DETECTION OF SARS-COV-2 NUCLEIC ACIDS IN 30 MINUTES
por: Tan, J., et al.
Publicado: (2023)

Human Adenovirus Subtype 21a Isolates From Children With Severe Lower Respiratory Illness in China
por: Liu, Wenkuan, et al.
Publicado: (2022)

Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B
por: Li, Xiao, et al.
Publicado: (2021)

Severe pneumonia and pathogenic damage in human airway epithelium caused by Coxsackievirus B4
por: Dai, Jing, et al.
Publicado: (2023)

Broadly neutralizing monoclonal antibodies against human adenovirus types 55, 14p, 7, and 11 generated with recombinant type 11 fiber knob
por: Tian, Xingui, et al.
Publicado: (2018)

Epidemiology and Genetic Variabilities of Human Adenovirus Type 55 Reveal Relative Genome Stability Across Time and Geographic Space in China
por: Chen, Shi-ying, et al.
Publicado: (2020)

Epidemiology and Clinical Presentations of Respiratory Syncytial Virus Subgroups A and B Detected with Multiplex Real-Time PCR
por: Liu, Wenkuan, et al.
Publicado: (2016)

Epidemiological and clinical features of human metapneumovirus in hospitalised paediatric patients with acute respiratory illness: a cross-sectional study in Southern China, from 2013 to 2016
por: Zhang, Ling, et al.
Publicado: (2018)

Protection against Enterovirus 71 with Neutralizing Epitope Incorporation within Adenovirus Type 3 Hexon
por: Tian, Xingui, et al.
Publicado: (2012)

Correction: Protection against Enterovirus 71 with Neutralizing Epitope Incorporation within Adenovirus Type 3 Hexon
por: Tian, Xingui, et al.
Publicado: (2012)

New Epidemiological and Clinical Signatures of 18 Pathogens from Respiratory Tract Infections Based on a 5-Year Study
por: Liao, Xiaohong, et al.
Publicado: (2015)

Establishment and Characterization of the First Chinese National Standard for Nucleic Acid Amplification Technology Assays for Hepatitis E Virus Nucleic Acid Detection
por: Li, Manyu, et al.
Publicado: (2023)

Development of two antigen-binding fragments to a conserved linear epitope of human adenovirus and their application in immunofluorescence
por: Liu, Zhenwei, et al.
Publicado: (2019)

Generation of Neutralizing Monoclonal Antibodies against a Conformational Epitope of Human Adenovirus Type 7 (HAdv-7) Incorporated in Capsid Encoded in a HAdv-3-Based Vector
por: Liu, Minglong, et al.
Publicado: (2014)

ESTABLISHMENT OF AN ULTRA HIGH-THROUGHPUT PROTEIN PRODUCTION PLATFORM (ULTRA 96) FOR EARLY STAGE IN VITRO STUDIES
por: Jiang, Kai, et al.
Publicado: (2023)

Two novel human coronavirus OC43 genotypes circulating in hospitalized children with pneumonia in China
por: Zhang, Zhaoyong, et al.
Publicado: (2022)

15-Minute nucleic acid test circles strategy in large cities in China
por: Li, Zhenjun, et al.
Publicado: (2022)

A 10-Day-Old Murine Model of Coxsackievirus A6 Infection for the Evaluation of Vaccines and Antiviral Drugs
por: Jiang, Zaixue, et al.
Publicado: (2021)

Seroprevalence of Neutralizing Antibodies against Six Human Adenovirus Types Indicates the Low Level of Herd Immunity in Young Children from Guangzhou, China
por: Tian, Xingui, et al.
Publicado: (2020)

R.F. minutes no. 30
por: Geibel, J
Publicado: (1959)

Nucleic Acids for Ultra-Sensitive Protein Detection
por: Janssen, Kris P. F., et al.
Publicado: (2013)

Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection
por: Jiang, Hao, et al.
Publicado: (2023)

Establishment of heterotopic ossification via sharp instrument injury in rats
por: Xu, J-C., et al.
Publicado: (2017)

An instrument-free, programmable approach for nucleic acid detection
Publicado: (2023)

Renal Tissue Thawed for 30 Minutes Is Still Suitable for Gene Expression Analysis
por: Ma, Yi, et al.
Publicado: (2014)

Boiling sheep liver or lung for 30 minutes is necessary and sufficient to kill Echinococcus granulosus protoscoleces in hydatid cysts
por: Li, Jun, et al.
Publicado: (2014)

Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus
por: Lu, Xu, et al.
Publicado: (2017)

Better punctuation in 30 minutes a day
por: Cleveland, Ceil
Publicado: (2002)

Better spelling in 30 minutes a day
por: Crosby, Harry, et al.
Publicado: (1999)

Minutes of the QCD Meeting on October 30, 1996
por: Cowan, G
Publicado: (1996)

Minutes of the meeting held on 30 1 87
por: Tejessy, W
Publicado: (1987)

Better grammar in 30 minutes a day
por: Immel, Constance, et al.
Publicado: (1995)

Establishment of a Simple and Rapid Nucleic Acid Detection Method for Hookworm Identification
por: Ding, Xin, et al.
Publicado: (2023)

SMART: On-Site Rapid Detection of Nucleic Acid from Plants, Animals, and Microorganisms in under 25 Minutes
por: Ma, Jun-Yuan, et al.
Publicado: (2023)

Complete genome analysis of a novel E3-partial-deleted human adenovirus type 7 strain isolated in Southern China
por: Su, Xiaobo, et al.
Publicado: (2011)

Minutes of APAL meeting no.5: Topic : AD instrumentation
por: Eriksson, T, et al.
Publicado: (1997)

Cannot write session to /tmp/vufind_sessions/sess_mb0g4n553dfs697edhq359i9ip