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Bilateral symmetry of linear streptomycete chromosomes

Here, we characterize an uncommon set of telomeres from Streptomyces rimosus ATCC 10970, the parental strain of a lineage of one of the earliest-discovered antibiotic producers. Following the closure of its genome sequence, we compared unusual telomeres from this organism with the other five classes...

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Autores principales: Algora-Gallardo, Lis, Schniete, Jana K., Mark, David R., Hunter, Iain S., Herron, Paul R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743542/
https://www.ncbi.nlm.nih.gov/pubmed/34779763
http://dx.doi.org/10.1099/mgen.0.000692
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author Algora-Gallardo, Lis
Schniete, Jana K.
Mark, David R.
Hunter, Iain S.
Herron, Paul R.
author_facet Algora-Gallardo, Lis
Schniete, Jana K.
Mark, David R.
Hunter, Iain S.
Herron, Paul R.
author_sort Algora-Gallardo, Lis
collection PubMed
description Here, we characterize an uncommon set of telomeres from Streptomyces rimosus ATCC 10970, the parental strain of a lineage of one of the earliest-discovered antibiotic producers. Following the closure of its genome sequence, we compared unusual telomeres from this organism with the other five classes of replicon ends found amongst streptomycetes. Closed replicons of streptomycete chromosomes were organized with respect to their phylogeny and physical orientation, which demonstrated that different telomeres were not associated with particular clades and are likely shared amongst different strains by plasmid-driven horizontal gene transfer. Furthermore, we identified a ~50 kb origin island with conserved synteny that is located at the core of all streptomycete chromosomes and forms an axis around which symmetrical chromosome inversions can take place. Despite this chromosomal bilateral symmetry, a bias in parS sites to the right of oriC is maintained across the family Streptomycetaceae and suggests that the formation of ParB/parS nucleoprotein complexes on the right replichore is a conserved feature in streptomycetes. Consequently, our studies reveal novel features of linear bacterial replicons that, through their manipulation, may lead to improvements in growth and productivity of this important industrial group of bacteria.
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spelling pubmed-87435422022-01-10 Bilateral symmetry of linear streptomycete chromosomes Algora-Gallardo, Lis Schniete, Jana K. Mark, David R. Hunter, Iain S. Herron, Paul R. Microb Genom Research Articles Here, we characterize an uncommon set of telomeres from Streptomyces rimosus ATCC 10970, the parental strain of a lineage of one of the earliest-discovered antibiotic producers. Following the closure of its genome sequence, we compared unusual telomeres from this organism with the other five classes of replicon ends found amongst streptomycetes. Closed replicons of streptomycete chromosomes were organized with respect to their phylogeny and physical orientation, which demonstrated that different telomeres were not associated with particular clades and are likely shared amongst different strains by plasmid-driven horizontal gene transfer. Furthermore, we identified a ~50 kb origin island with conserved synteny that is located at the core of all streptomycete chromosomes and forms an axis around which symmetrical chromosome inversions can take place. Despite this chromosomal bilateral symmetry, a bias in parS sites to the right of oriC is maintained across the family Streptomycetaceae and suggests that the formation of ParB/parS nucleoprotein complexes on the right replichore is a conserved feature in streptomycetes. Consequently, our studies reveal novel features of linear bacterial replicons that, through their manipulation, may lead to improvements in growth and productivity of this important industrial group of bacteria. Microbiology Society 2021-11-15 /pmc/articles/PMC8743542/ /pubmed/34779763 http://dx.doi.org/10.1099/mgen.0.000692 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Research Articles
Algora-Gallardo, Lis
Schniete, Jana K.
Mark, David R.
Hunter, Iain S.
Herron, Paul R.
Bilateral symmetry of linear streptomycete chromosomes
title Bilateral symmetry of linear streptomycete chromosomes
title_full Bilateral symmetry of linear streptomycete chromosomes
title_fullStr Bilateral symmetry of linear streptomycete chromosomes
title_full_unstemmed Bilateral symmetry of linear streptomycete chromosomes
title_short Bilateral symmetry of linear streptomycete chromosomes
title_sort bilateral symmetry of linear streptomycete chromosomes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743542/
https://www.ncbi.nlm.nih.gov/pubmed/34779763
http://dx.doi.org/10.1099/mgen.0.000692
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