Cargando…
Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation
We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, i...
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Microbiology Society
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743550/ https://www.ncbi.nlm.nih.gov/pubmed/34818145 http://dx.doi.org/10.1099/mgen.0.000687 |
| _version_ | 1784629927215104000 |
|---|---|
| author | Bossé, Janine T. Li, Yanwen Cohen, Liza Miriam Stegger, Marc Angen, Øystein Lacouture, Sonia Gottschalk, Marcelo Lei, Liancheng Koene, Miriam Kuhnert, Peter Bandara, Aloka B. Inzana, Thomas J. Holden, Matthew T. G. Harris, David Oshota, Olusegun Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. |
| author_facet | Bossé, Janine T. Li, Yanwen Cohen, Liza Miriam Stegger, Marc Angen, Øystein Lacouture, Sonia Gottschalk, Marcelo Lei, Liancheng Koene, Miriam Kuhnert, Peter Bandara, Aloka B. Inzana, Thomas J. Holden, Matthew T. G. Harris, David Oshota, Olusegun Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. |
| author_sort | Bossé, Janine T. |
| collection | PubMed |
| description | We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain. |
| format | Online Article Text |
| id | pubmed-8743550 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Microbiology Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-87435502022-01-10 Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation Bossé, Janine T. Li, Yanwen Cohen, Liza Miriam Stegger, Marc Angen, Øystein Lacouture, Sonia Gottschalk, Marcelo Lei, Liancheng Koene, Miriam Kuhnert, Peter Bandara, Aloka B. Inzana, Thomas J. Holden, Matthew T. G. Harris, David Oshota, Olusegun Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. Microb Genom Short Communications We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain. Microbiology Society 2021-11-24 /pmc/articles/PMC8743550/ /pubmed/34818145 http://dx.doi.org/10.1099/mgen.0.000687 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. |
| spellingShingle | Short Communications Bossé, Janine T. Li, Yanwen Cohen, Liza Miriam Stegger, Marc Angen, Øystein Lacouture, Sonia Gottschalk, Marcelo Lei, Liancheng Koene, Miriam Kuhnert, Peter Bandara, Aloka B. Inzana, Thomas J. Holden, Matthew T. G. Harris, David Oshota, Olusegun Maskell, Duncan J. Tucker, Alexander W. Wren, Brendan W. Rycroft, Andrew N. Langford, Paul R. Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation |
| title | Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation |
| title_full | Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation |
| title_fullStr | Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation |
| title_full_unstemmed | Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation |
| title_short | Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation |
| title_sort | complete genome for actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation |
| topic | Short Communications |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743550/ https://www.ncbi.nlm.nih.gov/pubmed/34818145 http://dx.doi.org/10.1099/mgen.0.000687 |
| work_keys_str_mv | AT bossejaninet completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT liyanwen completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT cohenlizamiriam completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT steggermarc completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT angenøystein completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT lacouturesonia completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT gottschalkmarcelo completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT leiliancheng completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT koenemiriam completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT kuhnertpeter completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT bandaraalokab completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT inzanathomasj completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT holdenmatthewtg completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT harrisdavid completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT oshotaolusegun completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT maskellduncanj completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT tuckeralexanderw completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT wrenbrendanw completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT rycroftandrewn completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT langfordpaulr completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation AT completegenomeforactinobacilluspleuropneumoniaeserovar8referencestrain405comparativeanalysiswithdraftgenomesfordifferentlaboratorystockculturesindicateslittlegeneticvariation |