Cargando…

Invasive atypical non-typhoidal Salmonella serovars in The Gambia

Invasive non-typhoidal Salmonella (iNTS) disease continues to be a significant public health problem in sub-Saharan Africa. Common clinical misdiagnosis, antimicrobial resistance, high case fatality and lack of a vaccine make iNTS a priority for global health research. Using whole genome sequence an...

Descripción completa

Detalles Bibliográficos
Autores principales: Kanteh, Abdoulie, Sesay, Abdul Karim, Alikhan, Nabil-Fareed, Ikumapayi, Usman Nurudeen, Salaudeen, Rasheed, Manneh, Jarra, Olatunji, Yekini, Page, Andrew J., Mackenzie, Grant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743563/
https://www.ncbi.nlm.nih.gov/pubmed/34812716
http://dx.doi.org/10.1099/mgen.0.000677
_version_ 1784629930446815232
author Kanteh, Abdoulie
Sesay, Abdul Karim
Alikhan, Nabil-Fareed
Ikumapayi, Usman Nurudeen
Salaudeen, Rasheed
Manneh, Jarra
Olatunji, Yekini
Page, Andrew J.
Mackenzie, Grant
author_facet Kanteh, Abdoulie
Sesay, Abdul Karim
Alikhan, Nabil-Fareed
Ikumapayi, Usman Nurudeen
Salaudeen, Rasheed
Manneh, Jarra
Olatunji, Yekini
Page, Andrew J.
Mackenzie, Grant
author_sort Kanteh, Abdoulie
collection PubMed
description Invasive non-typhoidal Salmonella (iNTS) disease continues to be a significant public health problem in sub-Saharan Africa. Common clinical misdiagnosis, antimicrobial resistance, high case fatality and lack of a vaccine make iNTS a priority for global health research. Using whole genome sequence analysis of 164 invasive Salmonella isolates obtained through population-based surveillance between 2008 and 2016, we conducted genomic analysis of the serovars causing invasive Salmonella diseases in rural Gambia. The incidence of iNTS varied over time. The proportion of atypical serovars causing disease increased over time from 40 to 65 % compared to the typical serovars Enteritidis and Typhimurium that decreased from 30 to 12 %. Overall iNTS case fatality was 10%, but case fatality associated with atypical iNTS alone was 10 %. Genetic virulence factors were identified in 14/70 (20 %) typical serovars and 45/68 (66 %) of the atypical serovars and were associated with: invasion, proliferation and/or translocation (Clade A); and host colonization and immune modulation (Clade G). Among Enteritidis isolates, 33/40 were resistant to four or more of the antimicrobials tested, except ciprofloxacin, to which all isolates were susceptible. Resistance was low in Typhimurium isolates, but all 16 isolates were resistant to gentamicin. The increase in incidence and proportion of iNTS disease caused by atypical serovars is concerning. The increased proportion of atypical serovars and the high associated case fatality may be related to acquisition of specific genetic virulence factors. These factors may provide a selective advantage to the atypical serovars. Investigations should be conducted elsewhere in Africa to identify potential changes in the distribution of iNTS serovars and the extent of these virulence elements.
format Online
Article
Text
id pubmed-8743563
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-87435632022-01-10 Invasive atypical non-typhoidal Salmonella serovars in The Gambia Kanteh, Abdoulie Sesay, Abdul Karim Alikhan, Nabil-Fareed Ikumapayi, Usman Nurudeen Salaudeen, Rasheed Manneh, Jarra Olatunji, Yekini Page, Andrew J. Mackenzie, Grant Microb Genom Research Articles Invasive non-typhoidal Salmonella (iNTS) disease continues to be a significant public health problem in sub-Saharan Africa. Common clinical misdiagnosis, antimicrobial resistance, high case fatality and lack of a vaccine make iNTS a priority for global health research. Using whole genome sequence analysis of 164 invasive Salmonella isolates obtained through population-based surveillance between 2008 and 2016, we conducted genomic analysis of the serovars causing invasive Salmonella diseases in rural Gambia. The incidence of iNTS varied over time. The proportion of atypical serovars causing disease increased over time from 40 to 65 % compared to the typical serovars Enteritidis and Typhimurium that decreased from 30 to 12 %. Overall iNTS case fatality was 10%, but case fatality associated with atypical iNTS alone was 10 %. Genetic virulence factors were identified in 14/70 (20 %) typical serovars and 45/68 (66 %) of the atypical serovars and were associated with: invasion, proliferation and/or translocation (Clade A); and host colonization and immune modulation (Clade G). Among Enteritidis isolates, 33/40 were resistant to four or more of the antimicrobials tested, except ciprofloxacin, to which all isolates were susceptible. Resistance was low in Typhimurium isolates, but all 16 isolates were resistant to gentamicin. The increase in incidence and proportion of iNTS disease caused by atypical serovars is concerning. The increased proportion of atypical serovars and the high associated case fatality may be related to acquisition of specific genetic virulence factors. These factors may provide a selective advantage to the atypical serovars. Investigations should be conducted elsewhere in Africa to identify potential changes in the distribution of iNTS serovars and the extent of these virulence elements. Microbiology Society 2021-11-23 /pmc/articles/PMC8743563/ /pubmed/34812716 http://dx.doi.org/10.1099/mgen.0.000677 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Research Articles
Kanteh, Abdoulie
Sesay, Abdul Karim
Alikhan, Nabil-Fareed
Ikumapayi, Usman Nurudeen
Salaudeen, Rasheed
Manneh, Jarra
Olatunji, Yekini
Page, Andrew J.
Mackenzie, Grant
Invasive atypical non-typhoidal Salmonella serovars in The Gambia
title Invasive atypical non-typhoidal Salmonella serovars in The Gambia
title_full Invasive atypical non-typhoidal Salmonella serovars in The Gambia
title_fullStr Invasive atypical non-typhoidal Salmonella serovars in The Gambia
title_full_unstemmed Invasive atypical non-typhoidal Salmonella serovars in The Gambia
title_short Invasive atypical non-typhoidal Salmonella serovars in The Gambia
title_sort invasive atypical non-typhoidal salmonella serovars in the gambia
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743563/
https://www.ncbi.nlm.nih.gov/pubmed/34812716
http://dx.doi.org/10.1099/mgen.0.000677
work_keys_str_mv AT kantehabdoulie invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia
AT sesayabdulkarim invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia
AT alikhannabilfareed invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia
AT ikumapayiusmannurudeen invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia
AT salaudeenrasheed invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia
AT mannehjarra invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia
AT olatunjiyekini invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia
AT pageandrewj invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia
AT mackenziegrant invasiveatypicalnontyphoidalsalmonellaserovarsinthegambia