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Phenotypical changes of satellite glial cells in a murine model of G(M1)‐gangliosidosis

Satellite glial cells (SGCs) of dorsal root ganglia (DRG) react in response to various injuries in the nervous system. This study investigates reactive changes within SGCs in a murine model for G(M1)‐gangliosidosis (G(M1)). DRG of homozygous β‐galactosidase‐knockout mice and homozygous C57BL/6 wild‐...

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Autores principales: Huang, Bei, Zdora, Isabel, de Buhr, Nicole, Eikelberg, Deborah, Baumgärtner, Wolfgang, Leitzen, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743646/
https://www.ncbi.nlm.nih.gov/pubmed/34877779
http://dx.doi.org/10.1111/jcmm.17113
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author Huang, Bei
Zdora, Isabel
de Buhr, Nicole
Eikelberg, Deborah
Baumgärtner, Wolfgang
Leitzen, Eva
author_facet Huang, Bei
Zdora, Isabel
de Buhr, Nicole
Eikelberg, Deborah
Baumgärtner, Wolfgang
Leitzen, Eva
author_sort Huang, Bei
collection PubMed
description Satellite glial cells (SGCs) of dorsal root ganglia (DRG) react in response to various injuries in the nervous system. This study investigates reactive changes within SGCs in a murine model for G(M1)‐gangliosidosis (G(M1)). DRG of homozygous β‐galactosidase‐knockout mice and homozygous C57BL/6 wild‐type mice were investigated performing immunostaining on formalin‐fixed, paraffin‐embedded tissue. A marked upregulation of glial fibrillary acidic protein (GFAP), the progenitor marker nestin and Ki67 within SGCs of diseased mice, starting after 4 months at the earliest GFAP, along with intracytoplasmic accumulation of ganglioside within neurons and deterioration of clinical signs was identified. Interestingly, nestin‐positive SGCs were detected after 8 months only. No changes regarding inwardly rectifying potassium channel 4.1, 2, 3‐cyclic nucleotide 3‐phosphodiesterase, Sox2, doublecortin, periaxin and caspase3 were observed in SGCs. Iba1 was only detected in close vicinity of SGCs indicating infiltrating or tissue‐resident macrophages. These results indicate that SGCs of DRG show phenotypical changes during the course of G(M1), characterized by GFAP upregulation, proliferation and expression of a neural progenitor marker at a late time point. This points towards an important role of SGCs during neurodegenerative disorders and supports that SGCs represent a multipotent glial precursor cell line with high plasticity and functionality.
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spelling pubmed-87436462022-01-12 Phenotypical changes of satellite glial cells in a murine model of G(M1)‐gangliosidosis Huang, Bei Zdora, Isabel de Buhr, Nicole Eikelberg, Deborah Baumgärtner, Wolfgang Leitzen, Eva J Cell Mol Med Original Articles Satellite glial cells (SGCs) of dorsal root ganglia (DRG) react in response to various injuries in the nervous system. This study investigates reactive changes within SGCs in a murine model for G(M1)‐gangliosidosis (G(M1)). DRG of homozygous β‐galactosidase‐knockout mice and homozygous C57BL/6 wild‐type mice were investigated performing immunostaining on formalin‐fixed, paraffin‐embedded tissue. A marked upregulation of glial fibrillary acidic protein (GFAP), the progenitor marker nestin and Ki67 within SGCs of diseased mice, starting after 4 months at the earliest GFAP, along with intracytoplasmic accumulation of ganglioside within neurons and deterioration of clinical signs was identified. Interestingly, nestin‐positive SGCs were detected after 8 months only. No changes regarding inwardly rectifying potassium channel 4.1, 2, 3‐cyclic nucleotide 3‐phosphodiesterase, Sox2, doublecortin, periaxin and caspase3 were observed in SGCs. Iba1 was only detected in close vicinity of SGCs indicating infiltrating or tissue‐resident macrophages. These results indicate that SGCs of DRG show phenotypical changes during the course of G(M1), characterized by GFAP upregulation, proliferation and expression of a neural progenitor marker at a late time point. This points towards an important role of SGCs during neurodegenerative disorders and supports that SGCs represent a multipotent glial precursor cell line with high plasticity and functionality. John Wiley and Sons Inc. 2021-12-07 2022-01 /pmc/articles/PMC8743646/ /pubmed/34877779 http://dx.doi.org/10.1111/jcmm.17113 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Huang, Bei
Zdora, Isabel
de Buhr, Nicole
Eikelberg, Deborah
Baumgärtner, Wolfgang
Leitzen, Eva
Phenotypical changes of satellite glial cells in a murine model of G(M1)‐gangliosidosis
title Phenotypical changes of satellite glial cells in a murine model of G(M1)‐gangliosidosis
title_full Phenotypical changes of satellite glial cells in a murine model of G(M1)‐gangliosidosis
title_fullStr Phenotypical changes of satellite glial cells in a murine model of G(M1)‐gangliosidosis
title_full_unstemmed Phenotypical changes of satellite glial cells in a murine model of G(M1)‐gangliosidosis
title_short Phenotypical changes of satellite glial cells in a murine model of G(M1)‐gangliosidosis
title_sort phenotypical changes of satellite glial cells in a murine model of g(m1)‐gangliosidosis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743646/
https://www.ncbi.nlm.nih.gov/pubmed/34877779
http://dx.doi.org/10.1111/jcmm.17113
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