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Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury

Monosodium glutamate (MSG) is a controversial food additive reported to cause negative effects on public health. Adipose stem cells (ASCs) and their derived vesicles (MVs) represent a promising cure for human diseases. This work was planned to compare the therapeutic effects of adipose stem cells an...

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Autores principales: Mazen, Nehad F., Abdel‐Fattah, Eman A., Desoky, Shimaa R., El‐Shal, Amal S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743657/
https://www.ncbi.nlm.nih.gov/pubmed/34874117
http://dx.doi.org/10.1111/jcmm.17083
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author Mazen, Nehad F.
Abdel‐Fattah, Eman A.
Desoky, Shimaa R.
El‐Shal, Amal S.
author_facet Mazen, Nehad F.
Abdel‐Fattah, Eman A.
Desoky, Shimaa R.
El‐Shal, Amal S.
author_sort Mazen, Nehad F.
collection PubMed
description Monosodium glutamate (MSG) is a controversial food additive reported to cause negative effects on public health. Adipose stem cells (ASCs) and their derived vesicles (MVs) represent a promising cure for human diseases. This work was planned to compare the therapeutic effects of adipose stem cells and microvesicles in MSG‐induced cerebellar damage. Forty adult healthy male Wister rats were equally divided into four groups: Group I (control group), group II (MSG‐treated), group III (MSG/ASCs‐treated), and group IV (MSG/MVs‐treated). Motor behaviour of rats was assessed. Characterization of ASCs and MVs was done by flow cytometry. The cerebellum was processed for light and electron microscopic studies, and immunohistochemical localization of PCNA and GFAP. Morphometry was done for the number of Purkinje cells in H&E‐stained sections, area per cent of GFAP immune reactivity and number of positive PCNA cells. Our results showed MSG‐induced deterioration in the motor part. Moreover, MSG increases oxidant and apoptotic with decreases of antioxidant biomarkers. Structural changes in the cerebellar cortex as degeneration of nerve cells and gliosis were detected. There were also a decrease in the number of Purkinje cells, an increase in the area per cent of GFAP immune reactivity and a decrease in the number of positive PCNA cells, as compared to the control. Rats treated with ASCs showed marked functional and structural improvement in comparison with MV‐treated rats. Thus, both ASCs and MVs had therapeutic potential for MSG‐induced cerebellar damage with better results in case of ASCs.
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spelling pubmed-87436572022-01-12 Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury Mazen, Nehad F. Abdel‐Fattah, Eman A. Desoky, Shimaa R. El‐Shal, Amal S. J Cell Mol Med Original Articles Monosodium glutamate (MSG) is a controversial food additive reported to cause negative effects on public health. Adipose stem cells (ASCs) and their derived vesicles (MVs) represent a promising cure for human diseases. This work was planned to compare the therapeutic effects of adipose stem cells and microvesicles in MSG‐induced cerebellar damage. Forty adult healthy male Wister rats were equally divided into four groups: Group I (control group), group II (MSG‐treated), group III (MSG/ASCs‐treated), and group IV (MSG/MVs‐treated). Motor behaviour of rats was assessed. Characterization of ASCs and MVs was done by flow cytometry. The cerebellum was processed for light and electron microscopic studies, and immunohistochemical localization of PCNA and GFAP. Morphometry was done for the number of Purkinje cells in H&E‐stained sections, area per cent of GFAP immune reactivity and number of positive PCNA cells. Our results showed MSG‐induced deterioration in the motor part. Moreover, MSG increases oxidant and apoptotic with decreases of antioxidant biomarkers. Structural changes in the cerebellar cortex as degeneration of nerve cells and gliosis were detected. There were also a decrease in the number of Purkinje cells, an increase in the area per cent of GFAP immune reactivity and a decrease in the number of positive PCNA cells, as compared to the control. Rats treated with ASCs showed marked functional and structural improvement in comparison with MV‐treated rats. Thus, both ASCs and MVs had therapeutic potential for MSG‐induced cerebellar damage with better results in case of ASCs. John Wiley and Sons Inc. 2021-12-07 2022-01 /pmc/articles/PMC8743657/ /pubmed/34874117 http://dx.doi.org/10.1111/jcmm.17083 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Mazen, Nehad F.
Abdel‐Fattah, Eman A.
Desoky, Shimaa R.
El‐Shal, Amal S.
Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury
title Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury
title_full Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury
title_fullStr Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury
title_full_unstemmed Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury
title_short Therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury
title_sort therapeutic role of adipose tissue–derived stem cells versus microvesicles in a rat model of cerebellar injury
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8743657/
https://www.ncbi.nlm.nih.gov/pubmed/34874117
http://dx.doi.org/10.1111/jcmm.17083
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