Cargando…

Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin

Traditional tissue engineering skin are composed of living cells and natural or synthetic scaffold. Besize the time delay and the risk of contamination involved with cell culture, the lack of autologous cell source and the persistence of allogeneic cells in heterologous grafts have limited its appli...

Descripción completa

Detalles Bibliográficos
Autores principales: Chang, Peng, Li, Shijie, Sun, Qian, Guo, Kai, Wang, Heran, Li, Song, Zhang, Liming, Xie, Yongbao, Zheng, Xiongfei, Liu, Yunhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744076/
https://www.ncbi.nlm.nih.gov/pubmed/35024135
http://dx.doi.org/10.1177/20417314211063022
_version_ 1784630044024373248
author Chang, Peng
Li, Shijie
Sun, Qian
Guo, Kai
Wang, Heran
Li, Song
Zhang, Liming
Xie, Yongbao
Zheng, Xiongfei
Liu, Yunhui
author_facet Chang, Peng
Li, Shijie
Sun, Qian
Guo, Kai
Wang, Heran
Li, Song
Zhang, Liming
Xie, Yongbao
Zheng, Xiongfei
Liu, Yunhui
author_sort Chang, Peng
collection PubMed
description Traditional tissue engineering skin are composed of living cells and natural or synthetic scaffold. Besize the time delay and the risk of contamination involved with cell culture, the lack of autologous cell source and the persistence of allogeneic cells in heterologous grafts have limited its application. This study shows a novel tissue engineering functional skin by carrying minimal functional unit of skin (MFUS) in 3D-printed polylactide-co-caprolactone (PLCL) scaffold and collagen gel (PLCL + Col + MFUS). MFUS is full-layer micro skin harvested from rat autologous tail skin. 3D-printed PLCL elastic scaffold has the similar mechanical properties with rat skin which provides a suitable environment for MFUS growing and enhances the skin wound healing. Four large full-thickness skin defects with 30 mm diameter of each wound are created in rat dorsal skin, and treated either with tissue engineering functional skin (PLCL + Col + MFUS), or with 3D-printed PLCL scaffold and collagen gel (PLCL + Col), or with micro skin islands only (Micro skin), or without treatment (Normal healing). The wound treated with PLCL + Col + MFUS heales much faster than the other three groups as evidenced by the fibroblasts migration from fascia to the gap between the MFUS dermis layer, and functional skin with hair follicles and sebaceous gland has been regenerated. The PLCL + Col treated wound heals faster than normal healing wound, but no skin appendages formed in PLCL + Col-treated wound. The wound treated with micro skin islands heals slower than the wounds treated either with tissue engineering skin (PLCL + Col + MFUS) or with PLCL + Col gel. Our results provide a new strategy to use autologous MFUS instead “seed cells” as the bio-resource of engineering skin for large full-thickness skin wound healing.
format Online
Article
Text
id pubmed-8744076
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-87440762022-01-11 Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin Chang, Peng Li, Shijie Sun, Qian Guo, Kai Wang, Heran Li, Song Zhang, Liming Xie, Yongbao Zheng, Xiongfei Liu, Yunhui J Tissue Eng Original Article Traditional tissue engineering skin are composed of living cells and natural or synthetic scaffold. Besize the time delay and the risk of contamination involved with cell culture, the lack of autologous cell source and the persistence of allogeneic cells in heterologous grafts have limited its application. This study shows a novel tissue engineering functional skin by carrying minimal functional unit of skin (MFUS) in 3D-printed polylactide-co-caprolactone (PLCL) scaffold and collagen gel (PLCL + Col + MFUS). MFUS is full-layer micro skin harvested from rat autologous tail skin. 3D-printed PLCL elastic scaffold has the similar mechanical properties with rat skin which provides a suitable environment for MFUS growing and enhances the skin wound healing. Four large full-thickness skin defects with 30 mm diameter of each wound are created in rat dorsal skin, and treated either with tissue engineering functional skin (PLCL + Col + MFUS), or with 3D-printed PLCL scaffold and collagen gel (PLCL + Col), or with micro skin islands only (Micro skin), or without treatment (Normal healing). The wound treated with PLCL + Col + MFUS heales much faster than the other three groups as evidenced by the fibroblasts migration from fascia to the gap between the MFUS dermis layer, and functional skin with hair follicles and sebaceous gland has been regenerated. The PLCL + Col treated wound heals faster than normal healing wound, but no skin appendages formed in PLCL + Col-treated wound. The wound treated with micro skin islands heals slower than the wounds treated either with tissue engineering skin (PLCL + Col + MFUS) or with PLCL + Col gel. Our results provide a new strategy to use autologous MFUS instead “seed cells” as the bio-resource of engineering skin for large full-thickness skin wound healing. SAGE Publications 2022-01-06 /pmc/articles/PMC8744076/ /pubmed/35024135 http://dx.doi.org/10.1177/20417314211063022 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Chang, Peng
Li, Shijie
Sun, Qian
Guo, Kai
Wang, Heran
Li, Song
Zhang, Liming
Xie, Yongbao
Zheng, Xiongfei
Liu, Yunhui
Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin
title Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin
title_full Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin
title_fullStr Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin
title_full_unstemmed Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin
title_short Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin
title_sort large full-thickness wounded skin regeneration using 3d-printed elastic scaffold with minimal functional unit of skin
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744076/
https://www.ncbi.nlm.nih.gov/pubmed/35024135
http://dx.doi.org/10.1177/20417314211063022
work_keys_str_mv AT changpeng largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT lishijie largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT sunqian largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT guokai largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT wangheran largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT lisong largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT zhangliming largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT xieyongbao largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT zhengxiongfei largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin
AT liuyunhui largefullthicknesswoundedskinregenerationusing3dprintedelasticscaffoldwithminimalfunctionalunitofskin