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Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SA...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Authors. Published by Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744407/ https://www.ncbi.nlm.nih.gov/pubmed/35032958 http://dx.doi.org/10.1016/j.jhazmat.2022.128251 |
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author | Tizaoui, Chedly Stanton, Richard Statkute, Evelina Rubina, Anzelika Lester-Card, Edward Lewis, Anthony Holliman, Peter Worsley, Dave |
author_facet | Tizaoui, Chedly Stanton, Richard Statkute, Evelina Rubina, Anzelika Lester-Card, Edward Lewis, Anthony Holliman, Peter Worsley, Dave |
author_sort | Tizaoui, Chedly |
collection | PubMed |
description | This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log(10)-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log(10)-reduction per ozone CT value(g min/m(3)) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19. |
format | Online Article Text |
id | pubmed-8744407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Authors. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87444072022-01-10 Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media Tizaoui, Chedly Stanton, Richard Statkute, Evelina Rubina, Anzelika Lester-Card, Edward Lewis, Anthony Holliman, Peter Worsley, Dave J Hazard Mater Research Paper This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log(10)-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log(10)-reduction per ozone CT value(g min/m(3)) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19. The Authors. Published by Elsevier B.V. 2022-04-15 2022-01-10 /pmc/articles/PMC8744407/ /pubmed/35032958 http://dx.doi.org/10.1016/j.jhazmat.2022.128251 Text en © 2022 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Research Paper Tizaoui, Chedly Stanton, Richard Statkute, Evelina Rubina, Anzelika Lester-Card, Edward Lewis, Anthony Holliman, Peter Worsley, Dave Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media |
title | Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media |
title_full | Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media |
title_fullStr | Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media |
title_full_unstemmed | Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media |
title_short | Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media |
title_sort | ozone for sars-cov-2 inactivation on surfaces and in liquid cell culture media |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744407/ https://www.ncbi.nlm.nih.gov/pubmed/35032958 http://dx.doi.org/10.1016/j.jhazmat.2022.128251 |
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