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Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study

Lead poisoning is often considered a traditional disease; however, the specific mechanism of toxicity remains unclear. The study of Pb-induced alterations in cellular metabolic pathways is important to understand the biological response and disorders associated with environmental exposure to lead. M...

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Autores principales: Nakata, Hokuto, Eguchi, Akifumi, Nakayama, Shouta M. M., Yabe, John, Muzandu, Kaampwe, Ikenaka, Yoshinori, Mori, Chisato, Ishizuka, Mayumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744629/
https://www.ncbi.nlm.nih.gov/pubmed/35010801
http://dx.doi.org/10.3390/ijerph19010541
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author Nakata, Hokuto
Eguchi, Akifumi
Nakayama, Shouta M. M.
Yabe, John
Muzandu, Kaampwe
Ikenaka, Yoshinori
Mori, Chisato
Ishizuka, Mayumi
author_facet Nakata, Hokuto
Eguchi, Akifumi
Nakayama, Shouta M. M.
Yabe, John
Muzandu, Kaampwe
Ikenaka, Yoshinori
Mori, Chisato
Ishizuka, Mayumi
author_sort Nakata, Hokuto
collection PubMed
description Lead poisoning is often considered a traditional disease; however, the specific mechanism of toxicity remains unclear. The study of Pb-induced alterations in cellular metabolic pathways is important to understand the biological response and disorders associated with environmental exposure to lead. Metabolomics studies have recently been paid considerable attention to understand in detail the biological response to lead exposure and the associated toxicity mechanisms. In the present study, wild rodents collected from an area contaminated with lead (N = 18) and a control area (N = 10) were investigated. This was the first ever experimental metabolomic study of wildlife exposed to lead in the field. While the levels of plasma phenylalanine and isoleucine were significantly higher in a lead-contaminated area versus the control area, hydroxybutyric acid was marginally significantly higher in the contaminated area, suggesting the possibility of enhancement of lipid metabolism. In the interregional least-absolute shrinkage and selection operator (lasso) regression model analysis, phenylalanine and isoleucine were identified as possible biomarkers, which is in agreement with the random forest model. In addition, in the random forest model, glutaric acid, glutamine, and hydroxybutyric acid were selected. In agreement with previous studies, enrichment analysis showed alterations in the urea cycle and ATP-binding cassette transporter pathways. Although regional rodent species bias was observed in this study, and the relatively small sample size should be taken into account, the present results are to some extent consistent with those of previous studies on humans and laboratory animals.
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spelling pubmed-87446292022-01-11 Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study Nakata, Hokuto Eguchi, Akifumi Nakayama, Shouta M. M. Yabe, John Muzandu, Kaampwe Ikenaka, Yoshinori Mori, Chisato Ishizuka, Mayumi Int J Environ Res Public Health Article Lead poisoning is often considered a traditional disease; however, the specific mechanism of toxicity remains unclear. The study of Pb-induced alterations in cellular metabolic pathways is important to understand the biological response and disorders associated with environmental exposure to lead. Metabolomics studies have recently been paid considerable attention to understand in detail the biological response to lead exposure and the associated toxicity mechanisms. In the present study, wild rodents collected from an area contaminated with lead (N = 18) and a control area (N = 10) were investigated. This was the first ever experimental metabolomic study of wildlife exposed to lead in the field. While the levels of plasma phenylalanine and isoleucine were significantly higher in a lead-contaminated area versus the control area, hydroxybutyric acid was marginally significantly higher in the contaminated area, suggesting the possibility of enhancement of lipid metabolism. In the interregional least-absolute shrinkage and selection operator (lasso) regression model analysis, phenylalanine and isoleucine were identified as possible biomarkers, which is in agreement with the random forest model. In addition, in the random forest model, glutaric acid, glutamine, and hydroxybutyric acid were selected. In agreement with previous studies, enrichment analysis showed alterations in the urea cycle and ATP-binding cassette transporter pathways. Although regional rodent species bias was observed in this study, and the relatively small sample size should be taken into account, the present results are to some extent consistent with those of previous studies on humans and laboratory animals. MDPI 2022-01-04 /pmc/articles/PMC8744629/ /pubmed/35010801 http://dx.doi.org/10.3390/ijerph19010541 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nakata, Hokuto
Eguchi, Akifumi
Nakayama, Shouta M. M.
Yabe, John
Muzandu, Kaampwe
Ikenaka, Yoshinori
Mori, Chisato
Ishizuka, Mayumi
Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study
title Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study
title_full Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study
title_fullStr Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study
title_full_unstemmed Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study
title_short Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study
title_sort metabolomic alteration in the plasma of wild rodents environmentally exposed to lead: a preliminary study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8744629/
https://www.ncbi.nlm.nih.gov/pubmed/35010801
http://dx.doi.org/10.3390/ijerph19010541
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