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Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths

The effects of visible light, from short to long wavelengths, on the retina were investigated functionally and histologically. The left eyes of Sprague–Dawley albino rats (6-weeks old, n = 6 for each wavelength) were exposed to seven narrow-band wavelengths (central wavelengths, 421, 441, 459, 501,...

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Autores principales: Kaidzu, Sachiko, Okuno, Tsutomu, Tanito, Masaki, Ohira, Akihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745104/
https://www.ncbi.nlm.nih.gov/pubmed/35008736
http://dx.doi.org/10.3390/ijms23010309
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author Kaidzu, Sachiko
Okuno, Tsutomu
Tanito, Masaki
Ohira, Akihiro
author_facet Kaidzu, Sachiko
Okuno, Tsutomu
Tanito, Masaki
Ohira, Akihiro
author_sort Kaidzu, Sachiko
collection PubMed
description The effects of visible light, from short to long wavelengths, on the retina were investigated functionally and histologically. The left eyes of Sprague–Dawley albino rats (6-weeks old, n = 6 for each wavelength) were exposed to seven narrow-band wavelengths (central wavelengths, 421, 441, 459, 501, 541, 581, and 615 nm) with bandwidths of 16 to 29 nm (half bandwidth, ±8–14.5 nm) using a xenon lamp source with bandpass filters at the retinal radiant exposures of 340 and 680 J/cm(2). The right unexposed eyes served as controls. Seven days after exposure, flash electroretinograms (ERGs) were recorded, and the outer nuclear layer (ONL) thickness was measured. Compared to the unexposed eyes, significant reductions in the a- and b-wave ERG amplitudes were seen in eyes exposed to 460-nm or shorter wavelengths of light. The ONL thickness near the optic nerve head also tended to decrease with exposure to shorter wavelengths. The decreased ERG amplitudes and ONL thicknesses were most prominent in eyes exposed to 420-nm light at both radiant exposures. When the wavelengths were the same, the higher the amount of radiant exposure and the stronger the damage. Compared to the unexposed eyes, the a- and b-waves did not decrease significantly in eyes exposed to 500-nm or longer wavelength light. The results indicate that the retinal damage induced by visible light observed in albino rats depends on the wavelength and energy level of the exposed light.
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spelling pubmed-87451042022-01-11 Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths Kaidzu, Sachiko Okuno, Tsutomu Tanito, Masaki Ohira, Akihiro Int J Mol Sci Article The effects of visible light, from short to long wavelengths, on the retina were investigated functionally and histologically. The left eyes of Sprague–Dawley albino rats (6-weeks old, n = 6 for each wavelength) were exposed to seven narrow-band wavelengths (central wavelengths, 421, 441, 459, 501, 541, 581, and 615 nm) with bandwidths of 16 to 29 nm (half bandwidth, ±8–14.5 nm) using a xenon lamp source with bandpass filters at the retinal radiant exposures of 340 and 680 J/cm(2). The right unexposed eyes served as controls. Seven days after exposure, flash electroretinograms (ERGs) were recorded, and the outer nuclear layer (ONL) thickness was measured. Compared to the unexposed eyes, significant reductions in the a- and b-wave ERG amplitudes were seen in eyes exposed to 460-nm or shorter wavelengths of light. The ONL thickness near the optic nerve head also tended to decrease with exposure to shorter wavelengths. The decreased ERG amplitudes and ONL thicknesses were most prominent in eyes exposed to 420-nm light at both radiant exposures. When the wavelengths were the same, the higher the amount of radiant exposure and the stronger the damage. Compared to the unexposed eyes, the a- and b-waves did not decrease significantly in eyes exposed to 500-nm or longer wavelength light. The results indicate that the retinal damage induced by visible light observed in albino rats depends on the wavelength and energy level of the exposed light. MDPI 2021-12-28 /pmc/articles/PMC8745104/ /pubmed/35008736 http://dx.doi.org/10.3390/ijms23010309 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kaidzu, Sachiko
Okuno, Tsutomu
Tanito, Masaki
Ohira, Akihiro
Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths
title Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths
title_full Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths
title_fullStr Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths
title_full_unstemmed Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths
title_short Structural and Functional Change in Albino Rat Retina Induced by Various Visible Light Wavelengths
title_sort structural and functional change in albino rat retina induced by various visible light wavelengths
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745104/
https://www.ncbi.nlm.nih.gov/pubmed/35008736
http://dx.doi.org/10.3390/ijms23010309
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