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Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR

Macrophage migration inhibitory factor (MIF) significantly contributes to rheumatoid arthritis (RA) pathogenesis. We aimed to evaluate the canonical (CD74/CD44) and non-canonical MIF receptors (CXCR2,4 and 7) expression and sCD74 to establish their association with RA clinical activity according to...

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Autores principales: Sánchez-Zuno, Gabriela Athziri, Bucala, Richard, Hernández-Bello, Jorge, Román-Fernández, Ilce Valeria, García-Chagollán, Mariel, Nicoletti, Ferdinando, Matuz-Flores, Mónica Guadalupe, García-Arellano, Samuel, Esparza-Michel, Judith Alejandra, Cerpa-Cruz, Sergio, Pérez-Guerrero, Edsaúl Emilio, Muñoz-Valle, José Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745239/
https://www.ncbi.nlm.nih.gov/pubmed/35011861
http://dx.doi.org/10.3390/jcm11010120
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author Sánchez-Zuno, Gabriela Athziri
Bucala, Richard
Hernández-Bello, Jorge
Román-Fernández, Ilce Valeria
García-Chagollán, Mariel
Nicoletti, Ferdinando
Matuz-Flores, Mónica Guadalupe
García-Arellano, Samuel
Esparza-Michel, Judith Alejandra
Cerpa-Cruz, Sergio
Pérez-Guerrero, Edsaúl Emilio
Muñoz-Valle, José Francisco
author_facet Sánchez-Zuno, Gabriela Athziri
Bucala, Richard
Hernández-Bello, Jorge
Román-Fernández, Ilce Valeria
García-Chagollán, Mariel
Nicoletti, Ferdinando
Matuz-Flores, Mónica Guadalupe
García-Arellano, Samuel
Esparza-Michel, Judith Alejandra
Cerpa-Cruz, Sergio
Pérez-Guerrero, Edsaúl Emilio
Muñoz-Valle, José Francisco
author_sort Sánchez-Zuno, Gabriela Athziri
collection PubMed
description Macrophage migration inhibitory factor (MIF) significantly contributes to rheumatoid arthritis (RA) pathogenesis. We aimed to evaluate the canonical (CD74/CD44) and non-canonical MIF receptors (CXCR2,4 and 7) expression and sCD74 to establish their association with RA clinical activity according to DAS28-ESR. Methodology: 101 RA patients with different clinical activities (remission (n = 27), low (n = 16), moderate (n = 35) and high (n = 23)) and 9 control subjects (CS) were included. Expression was evaluated by flow cytometry and levels of soluble CD74 (sCD74) by ELISA. Data analysis was performed with FlowJov10.0, STATAv12.0, and GraphPad Prism v7.0. Results: According to disease activity, CXCR7 expression (percentage of expression and mean fluorescence intensity (MFI)) was higher in granulocytes from patients in remission, while the expression of CXCR4 was higher in patients with high disease activity (p < 0.05). The expression of CD74 was higher in B cells (p < 0.05) and monocytes (p < 0.01) from patients in remission. Regarding sCD74 levels these were higher in patients with high disease activity when compared to those in remission (p <0.05). Conclusions: The results support the need for further study of the role of sCD74 as a soluble MIF decoy receptor, sequestering it to negatively regulate MIF signaling though its membrane receptors. The expression patterns of CXCR4 and CXCR7 show that the latter is a scavenger-type receptor that prevents endocytosis and even degradation of CXCR4 under inflammatory conditions.
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spelling pubmed-87452392022-01-11 Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR Sánchez-Zuno, Gabriela Athziri Bucala, Richard Hernández-Bello, Jorge Román-Fernández, Ilce Valeria García-Chagollán, Mariel Nicoletti, Ferdinando Matuz-Flores, Mónica Guadalupe García-Arellano, Samuel Esparza-Michel, Judith Alejandra Cerpa-Cruz, Sergio Pérez-Guerrero, Edsaúl Emilio Muñoz-Valle, José Francisco J Clin Med Article Macrophage migration inhibitory factor (MIF) significantly contributes to rheumatoid arthritis (RA) pathogenesis. We aimed to evaluate the canonical (CD74/CD44) and non-canonical MIF receptors (CXCR2,4 and 7) expression and sCD74 to establish their association with RA clinical activity according to DAS28-ESR. Methodology: 101 RA patients with different clinical activities (remission (n = 27), low (n = 16), moderate (n = 35) and high (n = 23)) and 9 control subjects (CS) were included. Expression was evaluated by flow cytometry and levels of soluble CD74 (sCD74) by ELISA. Data analysis was performed with FlowJov10.0, STATAv12.0, and GraphPad Prism v7.0. Results: According to disease activity, CXCR7 expression (percentage of expression and mean fluorescence intensity (MFI)) was higher in granulocytes from patients in remission, while the expression of CXCR4 was higher in patients with high disease activity (p < 0.05). The expression of CD74 was higher in B cells (p < 0.05) and monocytes (p < 0.01) from patients in remission. Regarding sCD74 levels these were higher in patients with high disease activity when compared to those in remission (p <0.05). Conclusions: The results support the need for further study of the role of sCD74 as a soluble MIF decoy receptor, sequestering it to negatively regulate MIF signaling though its membrane receptors. The expression patterns of CXCR4 and CXCR7 show that the latter is a scavenger-type receptor that prevents endocytosis and even degradation of CXCR4 under inflammatory conditions. MDPI 2021-12-27 /pmc/articles/PMC8745239/ /pubmed/35011861 http://dx.doi.org/10.3390/jcm11010120 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-Zuno, Gabriela Athziri
Bucala, Richard
Hernández-Bello, Jorge
Román-Fernández, Ilce Valeria
García-Chagollán, Mariel
Nicoletti, Ferdinando
Matuz-Flores, Mónica Guadalupe
García-Arellano, Samuel
Esparza-Michel, Judith Alejandra
Cerpa-Cruz, Sergio
Pérez-Guerrero, Edsaúl Emilio
Muñoz-Valle, José Francisco
Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR
title Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR
title_full Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR
title_fullStr Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR
title_full_unstemmed Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR
title_short Canonical (CD74/CD44) and Non-Canonical (CXCR2, 4 and 7) MIF Receptors Are Differentially Expressed in Rheumatoid Arthritis Patients Evaluated by DAS28-ESR
title_sort canonical (cd74/cd44) and non-canonical (cxcr2, 4 and 7) mif receptors are differentially expressed in rheumatoid arthritis patients evaluated by das28-esr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745239/
https://www.ncbi.nlm.nih.gov/pubmed/35011861
http://dx.doi.org/10.3390/jcm11010120
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