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Affinity and Specificity for Binding to Glycosaminoglycans Can Be Tuned by Adapting Peptide Length and Sequence

Although glycosaminoglycan (GAG)–protein interactions are important in many physiological and pathological processes, the structural requirements for binding are poorly defined. Starting with GAG-binding peptide CXCL9(74-103), peptides were designed to elucidate the contribution to the GAG-binding a...

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Autores principales: Crijns, Helena, Adyns, Lowie, Ganseman, Eva, Cambier, Seppe, Vandekerckhove, Eline, Pörtner, Noëmie, Vanbrabant, Lotte, Struyf, Sofie, Gerlza, Tanja, Kungl, Andreas, Proost, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745253/
https://www.ncbi.nlm.nih.gov/pubmed/35008874
http://dx.doi.org/10.3390/ijms23010447
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author Crijns, Helena
Adyns, Lowie
Ganseman, Eva
Cambier, Seppe
Vandekerckhove, Eline
Pörtner, Noëmie
Vanbrabant, Lotte
Struyf, Sofie
Gerlza, Tanja
Kungl, Andreas
Proost, Paul
author_facet Crijns, Helena
Adyns, Lowie
Ganseman, Eva
Cambier, Seppe
Vandekerckhove, Eline
Pörtner, Noëmie
Vanbrabant, Lotte
Struyf, Sofie
Gerlza, Tanja
Kungl, Andreas
Proost, Paul
author_sort Crijns, Helena
collection PubMed
description Although glycosaminoglycan (GAG)–protein interactions are important in many physiological and pathological processes, the structural requirements for binding are poorly defined. Starting with GAG-binding peptide CXCL9(74-103), peptides were designed to elucidate the contribution to the GAG-binding affinity of different: (1) GAG-binding motifs (i.e., BBXB and BBBXXB); (2) amino acids in GAG-binding motifs and linker sequences; and (3) numbers of GAG-binding motifs. The affinity of eight chemically synthesized peptides for various GAGs was determined by isothermal fluorescence titration (IFT). Moreover, the binding of peptides to cellular GAGs on Chinese hamster ovary (CHO) cells was assessed using flow cytometry with and without soluble GAGs. The repetition of GAG-binding motifs in the peptides contributed to a higher affinity for heparan sulfate (HS) in the IFT measurements. Furthermore, the presence of Gln residues in both GAG-binding motifs and linker sequences increased the affinity of trimer peptides for low-molecular-weight heparin (LMWH), partially desulfated (ds)LMWH and HS, but not for hyaluronic acid. In addition, the peptides bound to cellular GAGs with differential affinity, and the addition of soluble HS or heparin reduced the binding of CXCL9(74-103) to cellular GAGs. These results indicate that the affinity and specificity of peptides for GAGs can be tuned by adapting their amino acid sequence and their number of GAG-binding motifs.
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spelling pubmed-87452532022-01-11 Affinity and Specificity for Binding to Glycosaminoglycans Can Be Tuned by Adapting Peptide Length and Sequence Crijns, Helena Adyns, Lowie Ganseman, Eva Cambier, Seppe Vandekerckhove, Eline Pörtner, Noëmie Vanbrabant, Lotte Struyf, Sofie Gerlza, Tanja Kungl, Andreas Proost, Paul Int J Mol Sci Article Although glycosaminoglycan (GAG)–protein interactions are important in many physiological and pathological processes, the structural requirements for binding are poorly defined. Starting with GAG-binding peptide CXCL9(74-103), peptides were designed to elucidate the contribution to the GAG-binding affinity of different: (1) GAG-binding motifs (i.e., BBXB and BBBXXB); (2) amino acids in GAG-binding motifs and linker sequences; and (3) numbers of GAG-binding motifs. The affinity of eight chemically synthesized peptides for various GAGs was determined by isothermal fluorescence titration (IFT). Moreover, the binding of peptides to cellular GAGs on Chinese hamster ovary (CHO) cells was assessed using flow cytometry with and without soluble GAGs. The repetition of GAG-binding motifs in the peptides contributed to a higher affinity for heparan sulfate (HS) in the IFT measurements. Furthermore, the presence of Gln residues in both GAG-binding motifs and linker sequences increased the affinity of trimer peptides for low-molecular-weight heparin (LMWH), partially desulfated (ds)LMWH and HS, but not for hyaluronic acid. In addition, the peptides bound to cellular GAGs with differential affinity, and the addition of soluble HS or heparin reduced the binding of CXCL9(74-103) to cellular GAGs. These results indicate that the affinity and specificity of peptides for GAGs can be tuned by adapting their amino acid sequence and their number of GAG-binding motifs. MDPI 2021-12-31 /pmc/articles/PMC8745253/ /pubmed/35008874 http://dx.doi.org/10.3390/ijms23010447 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Crijns, Helena
Adyns, Lowie
Ganseman, Eva
Cambier, Seppe
Vandekerckhove, Eline
Pörtner, Noëmie
Vanbrabant, Lotte
Struyf, Sofie
Gerlza, Tanja
Kungl, Andreas
Proost, Paul
Affinity and Specificity for Binding to Glycosaminoglycans Can Be Tuned by Adapting Peptide Length and Sequence
title Affinity and Specificity for Binding to Glycosaminoglycans Can Be Tuned by Adapting Peptide Length and Sequence
title_full Affinity and Specificity for Binding to Glycosaminoglycans Can Be Tuned by Adapting Peptide Length and Sequence
title_fullStr Affinity and Specificity for Binding to Glycosaminoglycans Can Be Tuned by Adapting Peptide Length and Sequence
title_full_unstemmed Affinity and Specificity for Binding to Glycosaminoglycans Can Be Tuned by Adapting Peptide Length and Sequence
title_short Affinity and Specificity for Binding to Glycosaminoglycans Can Be Tuned by Adapting Peptide Length and Sequence
title_sort affinity and specificity for binding to glycosaminoglycans can be tuned by adapting peptide length and sequence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745253/
https://www.ncbi.nlm.nih.gov/pubmed/35008874
http://dx.doi.org/10.3390/ijms23010447
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