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Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L.
Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca(2+)](cyt)). Members of the Arabidopsis thaliana (L.) reduced hyperosmolality-gated calcium-permeable channels (OSCA) gene family are reported to be involved in sensing extracellular chang...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745296/ https://www.ncbi.nlm.nih.gov/pubmed/35008895 http://dx.doi.org/10.3390/ijms23010469 |
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author | Tong, Kai Wu, Xinyang He, Long Qiu, Shiyou Liu, Shuang Cai, Linna Rao, Shaofei Chen, Jianping |
author_facet | Tong, Kai Wu, Xinyang He, Long Qiu, Shiyou Liu, Shuang Cai, Linna Rao, Shaofei Chen, Jianping |
author_sort | Tong, Kai |
collection | PubMed |
description | Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca(2+)](cyt)). Members of the Arabidopsis thaliana (L.) reduced hyperosmolality-gated calcium-permeable channels (OSCA) gene family are reported to be involved in sensing extracellular changes to trigger hyperosmolality-induced [Ca(2+)](cyt) increases and controlling stomatal closure during immune signaling. Wheat (Triticum aestivum L.) is a very important food crop, but there are few studies of its OSCA gene family members. In this study, 42 OSCA members were identified in the wheat genome, and phylogenetic analysis can divide them into four clades. The members of each clade have similar gene structures, conserved motifs, and domains. TaOSCA genes were predicted to be regulated by cis-acting elements such as STRE, MBS, DRE1, ABRE, etc. Quantitative PCR results showed that they have different expression patterns in different tissues. The expression profiles of 15 selected TaOSCAs were examined after PEG (polyethylene glycol), NaCl, and ABA (abscisic acid) treatment. All 15 TaOSCA members responded to PEG treatment, while TaOSCA12/-39 responded simultaneously to PEG and ABA. This study informs research into the biological function and evolution of TaOSCA and lays the foundation for the breeding and genetic improvement of wheat. |
format | Online Article Text |
id | pubmed-8745296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87452962022-01-11 Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L. Tong, Kai Wu, Xinyang He, Long Qiu, Shiyou Liu, Shuang Cai, Linna Rao, Shaofei Chen, Jianping Int J Mol Sci Article Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca(2+)](cyt)). Members of the Arabidopsis thaliana (L.) reduced hyperosmolality-gated calcium-permeable channels (OSCA) gene family are reported to be involved in sensing extracellular changes to trigger hyperosmolality-induced [Ca(2+)](cyt) increases and controlling stomatal closure during immune signaling. Wheat (Triticum aestivum L.) is a very important food crop, but there are few studies of its OSCA gene family members. In this study, 42 OSCA members were identified in the wheat genome, and phylogenetic analysis can divide them into four clades. The members of each clade have similar gene structures, conserved motifs, and domains. TaOSCA genes were predicted to be regulated by cis-acting elements such as STRE, MBS, DRE1, ABRE, etc. Quantitative PCR results showed that they have different expression patterns in different tissues. The expression profiles of 15 selected TaOSCAs were examined after PEG (polyethylene glycol), NaCl, and ABA (abscisic acid) treatment. All 15 TaOSCA members responded to PEG treatment, while TaOSCA12/-39 responded simultaneously to PEG and ABA. This study informs research into the biological function and evolution of TaOSCA and lays the foundation for the breeding and genetic improvement of wheat. MDPI 2021-12-31 /pmc/articles/PMC8745296/ /pubmed/35008895 http://dx.doi.org/10.3390/ijms23010469 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tong, Kai Wu, Xinyang He, Long Qiu, Shiyou Liu, Shuang Cai, Linna Rao, Shaofei Chen, Jianping Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L. |
title | Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L. |
title_full | Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L. |
title_fullStr | Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L. |
title_full_unstemmed | Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L. |
title_short | Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L. |
title_sort | genome-wide identification and expression profile of osca gene family members in triticum aestivum l. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745296/ https://www.ncbi.nlm.nih.gov/pubmed/35008895 http://dx.doi.org/10.3390/ijms23010469 |
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