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CRISPR/Cas9 Genome Editing vs. Over-Expression for Fluorescent Extracellular Vesicle-Labeling: A Quantitative Analysis

Over-expression of fluorescently-labeled markers for extracellular vesicles is frequently used to visualize vesicle up-take and transport. EVs that are labeled by over-expression show considerable heterogeneity regarding the number of fluorophores on single particles, which could potentially bias tr...

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Detalles Bibliográficos
Autores principales:	Strohmeier, Karin, Hofmann, Martina, Hauser, Fabian, Sivun, Dmitry, Puthukodan, Sujitha, Karner, Andreas, Sandner, Georg, Le Renard, Pol-Edern, Jacak, Jaroslaw, Mairhofer, Mario
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745383/ https://www.ncbi.nlm.nih.gov/pubmed/35008709 http://dx.doi.org/10.3390/ijms23010282

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