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Two Novel Dimorphism-Related Virulence Factors of Zymoseptoria tritici Identified Using Agrobacterium-Mediated Insertional Mutagenesis

Diseases caused by dimorphic phytopathogenic and systemic dimorphic fungi have markedly increased in prevalence in the last decades, and understanding the morphogenic transition to the virulent state might yield novel means of controlling dimorphic fungi. The dimorphic fungus Z. tritici causes signi...

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Autores principales: Yemelin, Alexander, Brauchler, Annamaria, Jacob, Stefan, Foster, Andrew J., Laufer, Julian, Heck, Larissa, Antelo, Luis, Andresen, Karsten, Thines, Eckhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745584/
https://www.ncbi.nlm.nih.gov/pubmed/35008825
http://dx.doi.org/10.3390/ijms23010400
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author Yemelin, Alexander
Brauchler, Annamaria
Jacob, Stefan
Foster, Andrew J.
Laufer, Julian
Heck, Larissa
Antelo, Luis
Andresen, Karsten
Thines, Eckhard
author_facet Yemelin, Alexander
Brauchler, Annamaria
Jacob, Stefan
Foster, Andrew J.
Laufer, Julian
Heck, Larissa
Antelo, Luis
Andresen, Karsten
Thines, Eckhard
author_sort Yemelin, Alexander
collection PubMed
description Diseases caused by dimorphic phytopathogenic and systemic dimorphic fungi have markedly increased in prevalence in the last decades, and understanding the morphogenic transition to the virulent state might yield novel means of controlling dimorphic fungi. The dimorphic fungus Z. tritici causes significant economic impact on wheat production, and yet the regulation of the dimorphic switch, a key first step in successful plant colonization, is still largely unexplored in this fungus. The fungus is amenable to suppression by fungicides at this switch point, and the identification of the factors controlling the dimorphic switch provides a potential source of novel targets to control Septoria tritici blotch (STB). Inhibition of the dimorphic switch can potentially prevent penetration and avoid any damage to the host plant. The aim of the current work was to unveil genetic determinants of the dimorphic transition in Z. tritici by using a forward genetics strategy. Using this approach, we unveiled two novel factors involved in the switch to the pathogenic state and used reverse genetics and complementation to confirm the role of the novel virulence factors and further gained insight into the role of these genes, using transcriptome analysis via RNA-Seq. The transcriptomes generated potentially contain key determinants of the dimorphic transition.
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spelling pubmed-87455842022-01-11 Two Novel Dimorphism-Related Virulence Factors of Zymoseptoria tritici Identified Using Agrobacterium-Mediated Insertional Mutagenesis Yemelin, Alexander Brauchler, Annamaria Jacob, Stefan Foster, Andrew J. Laufer, Julian Heck, Larissa Antelo, Luis Andresen, Karsten Thines, Eckhard Int J Mol Sci Article Diseases caused by dimorphic phytopathogenic and systemic dimorphic fungi have markedly increased in prevalence in the last decades, and understanding the morphogenic transition to the virulent state might yield novel means of controlling dimorphic fungi. The dimorphic fungus Z. tritici causes significant economic impact on wheat production, and yet the regulation of the dimorphic switch, a key first step in successful plant colonization, is still largely unexplored in this fungus. The fungus is amenable to suppression by fungicides at this switch point, and the identification of the factors controlling the dimorphic switch provides a potential source of novel targets to control Septoria tritici blotch (STB). Inhibition of the dimorphic switch can potentially prevent penetration and avoid any damage to the host plant. The aim of the current work was to unveil genetic determinants of the dimorphic transition in Z. tritici by using a forward genetics strategy. Using this approach, we unveiled two novel factors involved in the switch to the pathogenic state and used reverse genetics and complementation to confirm the role of the novel virulence factors and further gained insight into the role of these genes, using transcriptome analysis via RNA-Seq. The transcriptomes generated potentially contain key determinants of the dimorphic transition. MDPI 2021-12-30 /pmc/articles/PMC8745584/ /pubmed/35008825 http://dx.doi.org/10.3390/ijms23010400 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yemelin, Alexander
Brauchler, Annamaria
Jacob, Stefan
Foster, Andrew J.
Laufer, Julian
Heck, Larissa
Antelo, Luis
Andresen, Karsten
Thines, Eckhard
Two Novel Dimorphism-Related Virulence Factors of Zymoseptoria tritici Identified Using Agrobacterium-Mediated Insertional Mutagenesis
title Two Novel Dimorphism-Related Virulence Factors of Zymoseptoria tritici Identified Using Agrobacterium-Mediated Insertional Mutagenesis
title_full Two Novel Dimorphism-Related Virulence Factors of Zymoseptoria tritici Identified Using Agrobacterium-Mediated Insertional Mutagenesis
title_fullStr Two Novel Dimorphism-Related Virulence Factors of Zymoseptoria tritici Identified Using Agrobacterium-Mediated Insertional Mutagenesis
title_full_unstemmed Two Novel Dimorphism-Related Virulence Factors of Zymoseptoria tritici Identified Using Agrobacterium-Mediated Insertional Mutagenesis
title_short Two Novel Dimorphism-Related Virulence Factors of Zymoseptoria tritici Identified Using Agrobacterium-Mediated Insertional Mutagenesis
title_sort two novel dimorphism-related virulence factors of zymoseptoria tritici identified using agrobacterium-mediated insertional mutagenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745584/
https://www.ncbi.nlm.nih.gov/pubmed/35008825
http://dx.doi.org/10.3390/ijms23010400
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