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Extracellular Nucleotides Affect the Proangiogenic Behavior of Fibroblasts, Keratinocytes, and Endothelial Cells
Chronic wound healing is currently a severe problem due to its incidence and associated complications. Intensive research is underway on substances that retain their biological activity in the wound microenvironment and stimulate the formation of new blood vessels critical for tissue regeneration. T...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745609/ https://www.ncbi.nlm.nih.gov/pubmed/35008664 http://dx.doi.org/10.3390/ijms23010238 |
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author | Węgłowska, Edyta Koziołkiewicz, Maria Kamińska, Daria Grobelski, Bartłomiej Pawełczak, Dariusz Kołodziejczyk, Marek Bielecki, Stanisław Gendaszewska-Darmach, Edyta |
author_facet | Węgłowska, Edyta Koziołkiewicz, Maria Kamińska, Daria Grobelski, Bartłomiej Pawełczak, Dariusz Kołodziejczyk, Marek Bielecki, Stanisław Gendaszewska-Darmach, Edyta |
author_sort | Węgłowska, Edyta |
collection | PubMed |
description | Chronic wound healing is currently a severe problem due to its incidence and associated complications. Intensive research is underway on substances that retain their biological activity in the wound microenvironment and stimulate the formation of new blood vessels critical for tissue regeneration. This group includes synthetic compounds with proangiogenic activity. Previously, we identified phosphorothioate analogs of nucleoside 5′-O-monophosphates as multifunctional ligands of P2Y6 and P2Y14 receptors. The effects of a series of unmodified and phosphorothioate nucleotide analogs on the secretion of VEGF from keratinocytes and fibroblasts, as well as their influence on the viability and proliferation of keratinocytes, fibroblasts, and endothelial cells were analyzed. In addition, the expression profiles of genes encoding nucleotide receptors in tested cell models were also investigated. In this study, we defined thymidine 5′-O-monophosphorothioate (TMPS) as a positive regulator of angiogenesis. Preliminary analyses confirmed the proangiogenic potency of TMPS in vivo. |
format | Online Article Text |
id | pubmed-8745609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87456092022-01-11 Extracellular Nucleotides Affect the Proangiogenic Behavior of Fibroblasts, Keratinocytes, and Endothelial Cells Węgłowska, Edyta Koziołkiewicz, Maria Kamińska, Daria Grobelski, Bartłomiej Pawełczak, Dariusz Kołodziejczyk, Marek Bielecki, Stanisław Gendaszewska-Darmach, Edyta Int J Mol Sci Article Chronic wound healing is currently a severe problem due to its incidence and associated complications. Intensive research is underway on substances that retain their biological activity in the wound microenvironment and stimulate the formation of new blood vessels critical for tissue regeneration. This group includes synthetic compounds with proangiogenic activity. Previously, we identified phosphorothioate analogs of nucleoside 5′-O-monophosphates as multifunctional ligands of P2Y6 and P2Y14 receptors. The effects of a series of unmodified and phosphorothioate nucleotide analogs on the secretion of VEGF from keratinocytes and fibroblasts, as well as their influence on the viability and proliferation of keratinocytes, fibroblasts, and endothelial cells were analyzed. In addition, the expression profiles of genes encoding nucleotide receptors in tested cell models were also investigated. In this study, we defined thymidine 5′-O-monophosphorothioate (TMPS) as a positive regulator of angiogenesis. Preliminary analyses confirmed the proangiogenic potency of TMPS in vivo. MDPI 2021-12-27 /pmc/articles/PMC8745609/ /pubmed/35008664 http://dx.doi.org/10.3390/ijms23010238 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Węgłowska, Edyta Koziołkiewicz, Maria Kamińska, Daria Grobelski, Bartłomiej Pawełczak, Dariusz Kołodziejczyk, Marek Bielecki, Stanisław Gendaszewska-Darmach, Edyta Extracellular Nucleotides Affect the Proangiogenic Behavior of Fibroblasts, Keratinocytes, and Endothelial Cells |
title | Extracellular Nucleotides Affect the Proangiogenic Behavior of Fibroblasts, Keratinocytes, and Endothelial Cells |
title_full | Extracellular Nucleotides Affect the Proangiogenic Behavior of Fibroblasts, Keratinocytes, and Endothelial Cells |
title_fullStr | Extracellular Nucleotides Affect the Proangiogenic Behavior of Fibroblasts, Keratinocytes, and Endothelial Cells |
title_full_unstemmed | Extracellular Nucleotides Affect the Proangiogenic Behavior of Fibroblasts, Keratinocytes, and Endothelial Cells |
title_short | Extracellular Nucleotides Affect the Proangiogenic Behavior of Fibroblasts, Keratinocytes, and Endothelial Cells |
title_sort | extracellular nucleotides affect the proangiogenic behavior of fibroblasts, keratinocytes, and endothelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745609/ https://www.ncbi.nlm.nih.gov/pubmed/35008664 http://dx.doi.org/10.3390/ijms23010238 |
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