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Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol
We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retri...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745613/ https://www.ncbi.nlm.nih.gov/pubmed/35008649 http://dx.doi.org/10.3390/ijms23010223 |
| _version_ | 1784630387476004864 |
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| author | von Schoenfeld, Anna Bronsert, Peter Poc, Michael Fuller, Andrew Filby, Andrew Kraft, Stefan Kurowski, Konrad Sörensen, Kristin Huber, Julia Pfeiffer, Jens Proietti, Michele Stehl, Verena Werner, Martin Seidl, Maximilian |
| author_facet | von Schoenfeld, Anna Bronsert, Peter Poc, Michael Fuller, Andrew Filby, Andrew Kraft, Stefan Kurowski, Konrad Sörensen, Kristin Huber, Julia Pfeiffer, Jens Proietti, Michele Stehl, Verena Werner, Martin Seidl, Maximilian |
| author_sort | von Schoenfeld, Anna |
| collection | PubMed |
| description | We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retrieval. Histomorphologic details are preserved, and crossreactivity and loss of signal intensity are not detectable. Combined with a DAB-based hydrophobic masking of metal-labeled primary antibodies, FOLGAS allows the extended use of CyTOF imaging in FFPE sections. |
| format | Online Article Text |
| id | pubmed-8745613 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | MDPI |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-87456132022-01-11 Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol von Schoenfeld, Anna Bronsert, Peter Poc, Michael Fuller, Andrew Filby, Andrew Kraft, Stefan Kurowski, Konrad Sörensen, Kristin Huber, Julia Pfeiffer, Jens Proietti, Michele Stehl, Verena Werner, Martin Seidl, Maximilian Int J Mol Sci Article We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retrieval. Histomorphologic details are preserved, and crossreactivity and loss of signal intensity are not detectable. Combined with a DAB-based hydrophobic masking of metal-labeled primary antibodies, FOLGAS allows the extended use of CyTOF imaging in FFPE sections. MDPI 2021-12-25 /pmc/articles/PMC8745613/ /pubmed/35008649 http://dx.doi.org/10.3390/ijms23010223 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Article von Schoenfeld, Anna Bronsert, Peter Poc, Michael Fuller, Andrew Filby, Andrew Kraft, Stefan Kurowski, Konrad Sörensen, Kristin Huber, Julia Pfeiffer, Jens Proietti, Michele Stehl, Verena Werner, Martin Seidl, Maximilian Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol |
| title | Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol |
| title_full | Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol |
| title_fullStr | Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol |
| title_full_unstemmed | Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol |
| title_short | Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol |
| title_sort | multiple immunostainings with different epitope retrievals—the folgas protocol |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745613/ https://www.ncbi.nlm.nih.gov/pubmed/35008649 http://dx.doi.org/10.3390/ijms23010223 |
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