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Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection

The worldwide increase in bacterial resistance and healthcare-associated bacterial infections pose a serious threat to human health. The antimicrobial photodynamic method reveals the opportunity for a new therapeutic approach that is based on the limited delivery of photosensitizer from the material...

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Autores principales: Buzalewicz, Igor, Hołowacz, Iwona, Matczuk, Anna K., Guźniczak, Mateusz, Skrzela, Dominika, Karwańska, Magdalena, Wieliczko, Alina, Kowal, Katarzyna, Ulatowska-Jarża, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745701/
https://www.ncbi.nlm.nih.gov/pubmed/35008705
http://dx.doi.org/10.3390/ijms23010279
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author Buzalewicz, Igor
Hołowacz, Iwona
Matczuk, Anna K.
Guźniczak, Mateusz
Skrzela, Dominika
Karwańska, Magdalena
Wieliczko, Alina
Kowal, Katarzyna
Ulatowska-Jarża, Agnieszka
author_facet Buzalewicz, Igor
Hołowacz, Iwona
Matczuk, Anna K.
Guźniczak, Mateusz
Skrzela, Dominika
Karwańska, Magdalena
Wieliczko, Alina
Kowal, Katarzyna
Ulatowska-Jarża, Agnieszka
author_sort Buzalewicz, Igor
collection PubMed
description The worldwide increase in bacterial resistance and healthcare-associated bacterial infections pose a serious threat to human health. The antimicrobial photodynamic method reveals the opportunity for a new therapeutic approach that is based on the limited delivery of photosensitizer from the material surface. Nanoporous inorganic–organic composites were obtained by entrapment of photosensitizer Photolon in polysiloxanes that was prepared by the sol–gel method. The material was characterized by its porosity, optical properties (fluorescence and absorbance), and laser-induced antimicrobial activity against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The permanent encapsulation of Photolon in the silica coating and the antimicrobial efficiency was confirmed by confocal microscope and digital holotomography. The generation of free radicals from nanoporous surfaces was proved by scanning Kelvin probe microscopy. For the first time, it was confirmed that Kelvin probe microscopy can be a label-free, noncontact alternative to other conventional methods based on fluorescence or chemiluminescence probes, etc. It was confirmed that the proposed photoactive coating enables the antibacterial photodynamic effect based on free radicals released from the surface of the coating. The highest bactericidal efficiency of the proposed coating was 87.16%. This coating can selectively limit the multiplication of bacterial cells, while protecting the environment and reducing the risk of surface contamination.
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spelling pubmed-87457012022-01-11 Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection Buzalewicz, Igor Hołowacz, Iwona Matczuk, Anna K. Guźniczak, Mateusz Skrzela, Dominika Karwańska, Magdalena Wieliczko, Alina Kowal, Katarzyna Ulatowska-Jarża, Agnieszka Int J Mol Sci Article The worldwide increase in bacterial resistance and healthcare-associated bacterial infections pose a serious threat to human health. The antimicrobial photodynamic method reveals the opportunity for a new therapeutic approach that is based on the limited delivery of photosensitizer from the material surface. Nanoporous inorganic–organic composites were obtained by entrapment of photosensitizer Photolon in polysiloxanes that was prepared by the sol–gel method. The material was characterized by its porosity, optical properties (fluorescence and absorbance), and laser-induced antimicrobial activity against Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The permanent encapsulation of Photolon in the silica coating and the antimicrobial efficiency was confirmed by confocal microscope and digital holotomography. The generation of free radicals from nanoporous surfaces was proved by scanning Kelvin probe microscopy. For the first time, it was confirmed that Kelvin probe microscopy can be a label-free, noncontact alternative to other conventional methods based on fluorescence or chemiluminescence probes, etc. It was confirmed that the proposed photoactive coating enables the antibacterial photodynamic effect based on free radicals released from the surface of the coating. The highest bactericidal efficiency of the proposed coating was 87.16%. This coating can selectively limit the multiplication of bacterial cells, while protecting the environment and reducing the risk of surface contamination. MDPI 2021-12-28 /pmc/articles/PMC8745701/ /pubmed/35008705 http://dx.doi.org/10.3390/ijms23010279 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Buzalewicz, Igor
Hołowacz, Iwona
Matczuk, Anna K.
Guźniczak, Mateusz
Skrzela, Dominika
Karwańska, Magdalena
Wieliczko, Alina
Kowal, Katarzyna
Ulatowska-Jarża, Agnieszka
Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection
title Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection
title_full Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection
title_fullStr Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection
title_full_unstemmed Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection
title_short Photolon Nanoporous Photoactive Material with Antibacterial Activity and Label-Free Noncontact Method for Free Radical Detection
title_sort photolon nanoporous photoactive material with antibacterial activity and label-free noncontact method for free radical detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745701/
https://www.ncbi.nlm.nih.gov/pubmed/35008705
http://dx.doi.org/10.3390/ijms23010279
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