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Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players

A deeper insight into the mechanisms responsible for athlete performance that may serve as specific and detailed training indicators is still desired, because conventionally used biomarkers provide limited information about the adaptive processes that occur during exercise. The objective of our stud...

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Autores principales: Podgórski, Rafał, Cieśla, Marek, Podgórska, Dominika, Bajorek, Wojciech, Płonka, Artur, Czarny, Wojciech, Trybulski, Robert, Król, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746094/
https://www.ncbi.nlm.nih.gov/pubmed/35012004
http://dx.doi.org/10.3390/jcm11010263
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author Podgórski, Rafał
Cieśla, Marek
Podgórska, Dominika
Bajorek, Wojciech
Płonka, Artur
Czarny, Wojciech
Trybulski, Robert
Król, Paweł
author_facet Podgórski, Rafał
Cieśla, Marek
Podgórska, Dominika
Bajorek, Wojciech
Płonka, Artur
Czarny, Wojciech
Trybulski, Robert
Król, Paweł
author_sort Podgórski, Rafał
collection PubMed
description A deeper insight into the mechanisms responsible for athlete performance that may serve as specific and detailed training indicators is still desired, because conventionally used biomarkers provide limited information about the adaptive processes that occur during exercise. The objective of our study was to assess insulin-like growth factor 1 receptors (IGF1R) gene expression and evaluate plasma concentration of selected microRNAs (miRNAs) during a 10-week training period (sampling times: week 1, 4, 7, and 10) in a group of 12 professional female volleyball players. Circulating miRNAs (miR-223, miR-320a, and miR-486) with established concentration in plasma and documented association with the IGF1 signaling pathway, which is involved in muscle development and recovery, were tested. The levels of analyzed miRNAs, tested by one-way ANOVA, were significantly different between four training periods during a 10-week training cycle (miR-223 p < 0.0001, miR-320a p = 0.00021, miR-486 p = 0.0037, respectively). The levels of IGF1R also appeared to be different (p = 0.00092), and their expression showed a trend to increase between the first and third periods. In the fourth period, the expression decreased, although it was higher compared with the baseline. Correlations between concentration levels of miR-223 and miR-320a (rs = 0.54, p < 0.001), as well as between miR-320a and miR-486 (rs = 0.73, p < 0.001) were also found. In the fourth period, a negative correlation between miR-223 plasma level and leucocyte IGF1R expression was found (rs = −0.63, p = 0.028). Multiple linear regression analysis showed that miR-320a (p = 0.024) and creatine kinase (p = 0.028) had the greatest impact on the expression levels of the IGF1R gene. Future studies are required to define whether these miRNAs, especially miR-320a, as well as IGF1R expression could be useful biomarkers of physiological changes during exercise and to discover their detailed biological roles in mode-specific exercise training adaptations of professional athletes.
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spelling pubmed-87460942022-01-11 Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players Podgórski, Rafał Cieśla, Marek Podgórska, Dominika Bajorek, Wojciech Płonka, Artur Czarny, Wojciech Trybulski, Robert Król, Paweł J Clin Med Article A deeper insight into the mechanisms responsible for athlete performance that may serve as specific and detailed training indicators is still desired, because conventionally used biomarkers provide limited information about the adaptive processes that occur during exercise. The objective of our study was to assess insulin-like growth factor 1 receptors (IGF1R) gene expression and evaluate plasma concentration of selected microRNAs (miRNAs) during a 10-week training period (sampling times: week 1, 4, 7, and 10) in a group of 12 professional female volleyball players. Circulating miRNAs (miR-223, miR-320a, and miR-486) with established concentration in plasma and documented association with the IGF1 signaling pathway, which is involved in muscle development and recovery, were tested. The levels of analyzed miRNAs, tested by one-way ANOVA, were significantly different between four training periods during a 10-week training cycle (miR-223 p < 0.0001, miR-320a p = 0.00021, miR-486 p = 0.0037, respectively). The levels of IGF1R also appeared to be different (p = 0.00092), and their expression showed a trend to increase between the first and third periods. In the fourth period, the expression decreased, although it was higher compared with the baseline. Correlations between concentration levels of miR-223 and miR-320a (rs = 0.54, p < 0.001), as well as between miR-320a and miR-486 (rs = 0.73, p < 0.001) were also found. In the fourth period, a negative correlation between miR-223 plasma level and leucocyte IGF1R expression was found (rs = −0.63, p = 0.028). Multiple linear regression analysis showed that miR-320a (p = 0.024) and creatine kinase (p = 0.028) had the greatest impact on the expression levels of the IGF1R gene. Future studies are required to define whether these miRNAs, especially miR-320a, as well as IGF1R expression could be useful biomarkers of physiological changes during exercise and to discover their detailed biological roles in mode-specific exercise training adaptations of professional athletes. MDPI 2022-01-05 /pmc/articles/PMC8746094/ /pubmed/35012004 http://dx.doi.org/10.3390/jcm11010263 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Podgórski, Rafał
Cieśla, Marek
Podgórska, Dominika
Bajorek, Wojciech
Płonka, Artur
Czarny, Wojciech
Trybulski, Robert
Król, Paweł
Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players
title Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players
title_full Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players
title_fullStr Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players
title_full_unstemmed Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players
title_short Plasma microRNA-320a as a Potential Biomarker of Physiological Changes during Training in Professional Volleyball Players
title_sort plasma microrna-320a as a potential biomarker of physiological changes during training in professional volleyball players
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746094/
https://www.ncbi.nlm.nih.gov/pubmed/35012004
http://dx.doi.org/10.3390/jcm11010263
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