Cargando…

Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification

Covalent crosslinks within or between proteins play a key role in determining the structure and function of proteins. Some of these are formed intentionally by either enzymatic or molecular reactions and are critical to normal physiological function. Others are generated as a consequence of exposure...

Descripción completa

Detalles Bibliográficos
Autores principales: Fuentes-Lemus, Eduardo, Hägglund, Per, López-Alarcón, Camilo, Davies, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746199/
https://www.ncbi.nlm.nih.gov/pubmed/35011250
http://dx.doi.org/10.3390/molecules27010015
_version_ 1784630527659081728
author Fuentes-Lemus, Eduardo
Hägglund, Per
López-Alarcón, Camilo
Davies, Michael J.
author_facet Fuentes-Lemus, Eduardo
Hägglund, Per
López-Alarcón, Camilo
Davies, Michael J.
author_sort Fuentes-Lemus, Eduardo
collection PubMed
description Covalent crosslinks within or between proteins play a key role in determining the structure and function of proteins. Some of these are formed intentionally by either enzymatic or molecular reactions and are critical to normal physiological function. Others are generated as a consequence of exposure to oxidants (radicals, excited states or two-electron species) and other endogenous or external stimuli, or as a result of the actions of a number of enzymes (e.g., oxidases and peroxidases). Increasing evidence indicates that the accumulation of unwanted crosslinks, as is seen in ageing and multiple pathologies, has adverse effects on biological function. In this article, we review the spectrum of crosslinks, both reducible and non-reducible, currently known to be formed on proteins; the mechanisms of their formation; and experimental approaches to the detection, identification and characterization of these species.
format Online
Article
Text
id pubmed-8746199
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87461992022-01-11 Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification Fuentes-Lemus, Eduardo Hägglund, Per López-Alarcón, Camilo Davies, Michael J. Molecules Review Covalent crosslinks within or between proteins play a key role in determining the structure and function of proteins. Some of these are formed intentionally by either enzymatic or molecular reactions and are critical to normal physiological function. Others are generated as a consequence of exposure to oxidants (radicals, excited states or two-electron species) and other endogenous or external stimuli, or as a result of the actions of a number of enzymes (e.g., oxidases and peroxidases). Increasing evidence indicates that the accumulation of unwanted crosslinks, as is seen in ageing and multiple pathologies, has adverse effects on biological function. In this article, we review the spectrum of crosslinks, both reducible and non-reducible, currently known to be formed on proteins; the mechanisms of their formation; and experimental approaches to the detection, identification and characterization of these species. MDPI 2021-12-21 /pmc/articles/PMC8746199/ /pubmed/35011250 http://dx.doi.org/10.3390/molecules27010015 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Fuentes-Lemus, Eduardo
Hägglund, Per
López-Alarcón, Camilo
Davies, Michael J.
Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification
title Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification
title_full Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification
title_fullStr Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification
title_full_unstemmed Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification
title_short Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification
title_sort oxidative crosslinking of peptides and proteins: mechanisms of formation, detection, characterization and quantification
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746199/
https://www.ncbi.nlm.nih.gov/pubmed/35011250
http://dx.doi.org/10.3390/molecules27010015
work_keys_str_mv AT fuenteslemuseduardo oxidativecrosslinkingofpeptidesandproteinsmechanismsofformationdetectioncharacterizationandquantification
AT hagglundper oxidativecrosslinkingofpeptidesandproteinsmechanismsofformationdetectioncharacterizationandquantification
AT lopezalarconcamilo oxidativecrosslinkingofpeptidesandproteinsmechanismsofformationdetectioncharacterizationandquantification
AT daviesmichaelj oxidativecrosslinkingofpeptidesandproteinsmechanismsofformationdetectioncharacterizationandquantification