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Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract

Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is ranked as the third most common cause of cancer-related mortality worldwide. Schinus molle (S. mole) L. is an important medicinal plant that contains many bioactive compounds with pharmacological properties. The role o...

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Autores principales: Hailan, Waleed Ali, Al-Anazi, Khalid Mashay, Farah, Mohammad Abul, Ali, Mohammad Ajmal, Al-Kawmani, Ahmed Ali, Abou-Tarboush, Faisal Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746381/
https://www.ncbi.nlm.nih.gov/pubmed/35010111
http://dx.doi.org/10.3390/nano12010161
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author Hailan, Waleed Ali
Al-Anazi, Khalid Mashay
Farah, Mohammad Abul
Ali, Mohammad Ajmal
Al-Kawmani, Ahmed Ali
Abou-Tarboush, Faisal Mohammed
author_facet Hailan, Waleed Ali
Al-Anazi, Khalid Mashay
Farah, Mohammad Abul
Ali, Mohammad Ajmal
Al-Kawmani, Ahmed Ali
Abou-Tarboush, Faisal Mohammed
author_sort Hailan, Waleed Ali
collection PubMed
description Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is ranked as the third most common cause of cancer-related mortality worldwide. Schinus molle (S. mole) L. is an important medicinal plant that contains many bioactive compounds with pharmacological properties. The role of S. molle leaf extract in the biosynthesis of silver nanoparticles (AgNPs) was determined. The biosynthesized AgNPs were thoroughly characterized by UV–vis spectrophotometry, transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) techniques. Furthermore, the cytotoxic effect of the biosynthesized AgNPs using S. molle (SMAgNPs) against HepG2 liver cancer cells was investigated. Reactive oxygen species generation, apoptosis induction, DNA damage, and autophagy activity were analyzed. The results clearly showed that the biosynthesized silver nanoparticles inhibited the proliferation of HepG2 by significantly (p < 0.05) inducing oxidative stress, cytotoxicity, DNA damage, apoptosis, and autophagy in a dose- and time-dependent manner. These findings may encourage integrating the potential of natural products and the efficiency of silver nanoparticles for the fabrication of safe, environmentally friendly, and effective anticancer agents.
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spelling pubmed-87463812022-01-11 Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract Hailan, Waleed Ali Al-Anazi, Khalid Mashay Farah, Mohammad Abul Ali, Mohammad Ajmal Al-Kawmani, Ahmed Ali Abou-Tarboush, Faisal Mohammed Nanomaterials (Basel) Article Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is ranked as the third most common cause of cancer-related mortality worldwide. Schinus molle (S. mole) L. is an important medicinal plant that contains many bioactive compounds with pharmacological properties. The role of S. molle leaf extract in the biosynthesis of silver nanoparticles (AgNPs) was determined. The biosynthesized AgNPs were thoroughly characterized by UV–vis spectrophotometry, transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) techniques. Furthermore, the cytotoxic effect of the biosynthesized AgNPs using S. molle (SMAgNPs) against HepG2 liver cancer cells was investigated. Reactive oxygen species generation, apoptosis induction, DNA damage, and autophagy activity were analyzed. The results clearly showed that the biosynthesized silver nanoparticles inhibited the proliferation of HepG2 by significantly (p < 0.05) inducing oxidative stress, cytotoxicity, DNA damage, apoptosis, and autophagy in a dose- and time-dependent manner. These findings may encourage integrating the potential of natural products and the efficiency of silver nanoparticles for the fabrication of safe, environmentally friendly, and effective anticancer agents. MDPI 2022-01-03 /pmc/articles/PMC8746381/ /pubmed/35010111 http://dx.doi.org/10.3390/nano12010161 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hailan, Waleed Ali
Al-Anazi, Khalid Mashay
Farah, Mohammad Abul
Ali, Mohammad Ajmal
Al-Kawmani, Ahmed Ali
Abou-Tarboush, Faisal Mohammed
Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract
title Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract
title_full Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract
title_fullStr Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract
title_full_unstemmed Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract
title_short Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract
title_sort reactive oxygen species-mediated cytotoxicity in liver carcinoma cells induced by silver nanoparticles biosynthesized using schinus molle extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746381/
https://www.ncbi.nlm.nih.gov/pubmed/35010111
http://dx.doi.org/10.3390/nano12010161
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