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Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway
Intestinal melatonin exerts diverse biological effects on the body. Our previous research showed that the abundance of the butyrate-producing bacteria, Roseburia, is positively related to the expression of colonic mucosal melatonin. However, the detailed relationship is unclear. Therefore, we aimed...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746519/ https://www.ncbi.nlm.nih.gov/pubmed/35010992 http://dx.doi.org/10.3390/nu14010117 |
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author | Song, Lijin He, Meibo Sun, Qinghua Wang, Yujing Zhang, Jindong Fang, Yuan Liu, Shuangjiang Duan, Liping |
author_facet | Song, Lijin He, Meibo Sun, Qinghua Wang, Yujing Zhang, Jindong Fang, Yuan Liu, Shuangjiang Duan, Liping |
author_sort | Song, Lijin |
collection | PubMed |
description | Intestinal melatonin exerts diverse biological effects on the body. Our previous research showed that the abundance of the butyrate-producing bacteria, Roseburia, is positively related to the expression of colonic mucosal melatonin. However, the detailed relationship is unclear. Therefore, we aimed to explore whether Roseburia regulates intestinal melatonin and its underlying mechanisms. Male Sprague–Dawley germfree rats were orally administered with or without Roseburia hominis. R. hominis treatment significantly increased the intestinal melatonin level. The concentrations of propionate and butyrate in the intestinal contents were significantly elevated after gavage of R. hominis. Propionate or butyrate treatment increased melatonin, 5-hydroxytryptamine (5-HT), arylalkylamine N-acetyltransferase (AANAT), and phosphorylated cAMP-response element-binding protein (p-CREB) levels. When pretreated with telotristat ethyl, the inhibitor of tryptophan hydroxylase (TPH), or siRNA of Aanat, or 666-15, i.e., an inhibitor of CREB, propionate, or butyrate, could not promote melatonin production in the pheochromocytoma cell line BON-1. Metabolomics analysis showed that propionate and butyrate stimulation regulated levels of some metabolites and some metabolic pathways in BON-1 cell supernatants. In conclusion, propionate and butyrate, i.e., metabolites of R. hominis, can promote intestinal melatonin synthesis by increasing 5-HT levels and promoting p-CREB-mediated Aanat transcription, thereby offering a potential target for ameliorating intestinal diseases. |
format | Online Article Text |
id | pubmed-8746519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87465192022-01-11 Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway Song, Lijin He, Meibo Sun, Qinghua Wang, Yujing Zhang, Jindong Fang, Yuan Liu, Shuangjiang Duan, Liping Nutrients Article Intestinal melatonin exerts diverse biological effects on the body. Our previous research showed that the abundance of the butyrate-producing bacteria, Roseburia, is positively related to the expression of colonic mucosal melatonin. However, the detailed relationship is unclear. Therefore, we aimed to explore whether Roseburia regulates intestinal melatonin and its underlying mechanisms. Male Sprague–Dawley germfree rats were orally administered with or without Roseburia hominis. R. hominis treatment significantly increased the intestinal melatonin level. The concentrations of propionate and butyrate in the intestinal contents were significantly elevated after gavage of R. hominis. Propionate or butyrate treatment increased melatonin, 5-hydroxytryptamine (5-HT), arylalkylamine N-acetyltransferase (AANAT), and phosphorylated cAMP-response element-binding protein (p-CREB) levels. When pretreated with telotristat ethyl, the inhibitor of tryptophan hydroxylase (TPH), or siRNA of Aanat, or 666-15, i.e., an inhibitor of CREB, propionate, or butyrate, could not promote melatonin production in the pheochromocytoma cell line BON-1. Metabolomics analysis showed that propionate and butyrate stimulation regulated levels of some metabolites and some metabolic pathways in BON-1 cell supernatants. In conclusion, propionate and butyrate, i.e., metabolites of R. hominis, can promote intestinal melatonin synthesis by increasing 5-HT levels and promoting p-CREB-mediated Aanat transcription, thereby offering a potential target for ameliorating intestinal diseases. MDPI 2021-12-28 /pmc/articles/PMC8746519/ /pubmed/35010992 http://dx.doi.org/10.3390/nu14010117 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Song, Lijin He, Meibo Sun, Qinghua Wang, Yujing Zhang, Jindong Fang, Yuan Liu, Shuangjiang Duan, Liping Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway |
title | Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway |
title_full | Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway |
title_fullStr | Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway |
title_full_unstemmed | Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway |
title_short | Roseburia hominis Increases Intestinal Melatonin Level by Activating p-CREB-AANAT Pathway |
title_sort | roseburia hominis increases intestinal melatonin level by activating p-creb-aanat pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746519/ https://www.ncbi.nlm.nih.gov/pubmed/35010992 http://dx.doi.org/10.3390/nu14010117 |
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