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Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells

Uropathogenic Escherichia coli (UPEC) is the most common pathogenic bacteria associated with urinary tract infection (UTI). UPEC can cause UTI by adhering to and invading uroepithelial cells. Fimbriae is the most important virulence factor of UPEC, and a potentially promising target in developing no...

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Autores principales: Yang, Wenbo, Liu, Peng, Chen, Ying, Lv, Qingyu, Wang, Zhongtian, Huang, Wenhua, Jiang, Hua, Zheng, Yuling, Jiang, Yongqiang, Sun, Liping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746591/
https://www.ncbi.nlm.nih.gov/pubmed/35011504
http://dx.doi.org/10.3390/molecules27010272
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author Yang, Wenbo
Liu, Peng
Chen, Ying
Lv, Qingyu
Wang, Zhongtian
Huang, Wenhua
Jiang, Hua
Zheng, Yuling
Jiang, Yongqiang
Sun, Liping
author_facet Yang, Wenbo
Liu, Peng
Chen, Ying
Lv, Qingyu
Wang, Zhongtian
Huang, Wenhua
Jiang, Hua
Zheng, Yuling
Jiang, Yongqiang
Sun, Liping
author_sort Yang, Wenbo
collection PubMed
description Uropathogenic Escherichia coli (UPEC) is the most common pathogenic bacteria associated with urinary tract infection (UTI). UPEC can cause UTI by adhering to and invading uroepithelial cells. Fimbriae is the most important virulence factor of UPEC, and a potentially promising target in developing novel antibacterial treatments. In this study, the antibacterial properties and effects of the compound dictamnine, extracted from the traditional Chinese medicine Cortex Dictamni, on the bacterial morphology, cell adhesion, and invasion of UPEC were studied. Dictamnine exhibited no obvious antibacterial activity against UPEC, but significantly impeded the ability of UPEC to adhere to and invade uroepithelial cells. RT-qPCR analysis showed that treatment downregulated the expression of type 1 fimbriae, P fimbriae, and curli fimbriae adhesion genes, and also downregulated adhesion-related receptor genes of uroepithelial cells. Transmission electron microscopy showed that dictamnine destroyed the structure of the fimbriae and the surface of the bacteria became smooth. These results suggest that dictamnine may help to prevent UTI by simultaneously targeting UPEC fimbriae and urothelial adhesin receptors, and may have a potential use as a new anti-UPEC drug.
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spelling pubmed-87465912022-01-11 Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells Yang, Wenbo Liu, Peng Chen, Ying Lv, Qingyu Wang, Zhongtian Huang, Wenhua Jiang, Hua Zheng, Yuling Jiang, Yongqiang Sun, Liping Molecules Article Uropathogenic Escherichia coli (UPEC) is the most common pathogenic bacteria associated with urinary tract infection (UTI). UPEC can cause UTI by adhering to and invading uroepithelial cells. Fimbriae is the most important virulence factor of UPEC, and a potentially promising target in developing novel antibacterial treatments. In this study, the antibacterial properties and effects of the compound dictamnine, extracted from the traditional Chinese medicine Cortex Dictamni, on the bacterial morphology, cell adhesion, and invasion of UPEC were studied. Dictamnine exhibited no obvious antibacterial activity against UPEC, but significantly impeded the ability of UPEC to adhere to and invade uroepithelial cells. RT-qPCR analysis showed that treatment downregulated the expression of type 1 fimbriae, P fimbriae, and curli fimbriae adhesion genes, and also downregulated adhesion-related receptor genes of uroepithelial cells. Transmission electron microscopy showed that dictamnine destroyed the structure of the fimbriae and the surface of the bacteria became smooth. These results suggest that dictamnine may help to prevent UTI by simultaneously targeting UPEC fimbriae and urothelial adhesin receptors, and may have a potential use as a new anti-UPEC drug. MDPI 2022-01-02 /pmc/articles/PMC8746591/ /pubmed/35011504 http://dx.doi.org/10.3390/molecules27010272 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Wenbo
Liu, Peng
Chen, Ying
Lv, Qingyu
Wang, Zhongtian
Huang, Wenhua
Jiang, Hua
Zheng, Yuling
Jiang, Yongqiang
Sun, Liping
Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells
title Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells
title_full Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells
title_fullStr Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells
title_full_unstemmed Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells
title_short Dictamnine Inhibits the Adhesion to and Invasion of Uropathogenic Escherichia Coli (UPEC) to Urothelial Cells
title_sort dictamnine inhibits the adhesion to and invasion of uropathogenic escherichia coli (upec) to urothelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746591/
https://www.ncbi.nlm.nih.gov/pubmed/35011504
http://dx.doi.org/10.3390/molecules27010272
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