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Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria

We have developed a convenient and selective method for the detection of Gram-positive bacteria using a ditopic poly(amidoamine) (PAMAM) dendrimer probe. The dendrimer that was modified with dipicolylamine (dpa) and phenylboronic acid groups showed selectivity toward Staphylococcus aureus. The ditop...

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Autores principales: Mikagi, Ayame, Manita, Koichi, Yoyasu, Asuka, Tsuchido, Yuji, Kanzawa, Nobuyuki, Hashimoto, Takeshi, Hayashita, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746651/
https://www.ncbi.nlm.nih.gov/pubmed/35011488
http://dx.doi.org/10.3390/molecules27010256
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author Mikagi, Ayame
Manita, Koichi
Yoyasu, Asuka
Tsuchido, Yuji
Kanzawa, Nobuyuki
Hashimoto, Takeshi
Hayashita, Takashi
author_facet Mikagi, Ayame
Manita, Koichi
Yoyasu, Asuka
Tsuchido, Yuji
Kanzawa, Nobuyuki
Hashimoto, Takeshi
Hayashita, Takashi
author_sort Mikagi, Ayame
collection PubMed
description We have developed a convenient and selective method for the detection of Gram-positive bacteria using a ditopic poly(amidoamine) (PAMAM) dendrimer probe. The dendrimer that was modified with dipicolylamine (dpa) and phenylboronic acid groups showed selectivity toward Staphylococcus aureus. The ditopic dendrimer system had higher sensitivity and better pH tolerance than the monotopic PAMAM dendrimer probe. We also investigated the mechanisms of various ditopic PAMAM dendrimer probes and found that the selectivity toward Gram-positive bacteria was dependent on a variety of interactions. Supramolecular interactions, such as electrostatic interaction and hydrophobic interaction, per se, did not contribute to the bacterial recognition ability, nor did they improve the selectivity of the ditopic dendrimer system. In contrast, the ditopic PAMAM dendrimer probe that had a phosphate-sensing dpa group and formed a chelate with metal ions showed improved selectivity toward S. aureus. The results suggested that the targeted ditopic PAMAM dendrimer probe showed selectivity toward Gram-positive bacteria. This study is expected to contribute to the elucidation of the interaction between synthetic molecules and bacterial surface. Moreover, our novel method showed potential for the rapid and species-specific recognition of various bacteria.
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spelling pubmed-87466512022-01-11 Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria Mikagi, Ayame Manita, Koichi Yoyasu, Asuka Tsuchido, Yuji Kanzawa, Nobuyuki Hashimoto, Takeshi Hayashita, Takashi Molecules Article We have developed a convenient and selective method for the detection of Gram-positive bacteria using a ditopic poly(amidoamine) (PAMAM) dendrimer probe. The dendrimer that was modified with dipicolylamine (dpa) and phenylboronic acid groups showed selectivity toward Staphylococcus aureus. The ditopic dendrimer system had higher sensitivity and better pH tolerance than the monotopic PAMAM dendrimer probe. We also investigated the mechanisms of various ditopic PAMAM dendrimer probes and found that the selectivity toward Gram-positive bacteria was dependent on a variety of interactions. Supramolecular interactions, such as electrostatic interaction and hydrophobic interaction, per se, did not contribute to the bacterial recognition ability, nor did they improve the selectivity of the ditopic dendrimer system. In contrast, the ditopic PAMAM dendrimer probe that had a phosphate-sensing dpa group and formed a chelate with metal ions showed improved selectivity toward S. aureus. The results suggested that the targeted ditopic PAMAM dendrimer probe showed selectivity toward Gram-positive bacteria. This study is expected to contribute to the elucidation of the interaction between synthetic molecules and bacterial surface. Moreover, our novel method showed potential for the rapid and species-specific recognition of various bacteria. MDPI 2021-12-31 /pmc/articles/PMC8746651/ /pubmed/35011488 http://dx.doi.org/10.3390/molecules27010256 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mikagi, Ayame
Manita, Koichi
Yoyasu, Asuka
Tsuchido, Yuji
Kanzawa, Nobuyuki
Hashimoto, Takeshi
Hayashita, Takashi
Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria
title Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria
title_full Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria
title_fullStr Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria
title_full_unstemmed Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria
title_short Rapid Bacterial Recognition over a Wide pH Range by Boronic Acid-Based Ditopic Dendrimer Probes for Gram-Positive Bacteria
title_sort rapid bacterial recognition over a wide ph range by boronic acid-based ditopic dendrimer probes for gram-positive bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746651/
https://www.ncbi.nlm.nih.gov/pubmed/35011488
http://dx.doi.org/10.3390/molecules27010256
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