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Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut–Immune System–Brain Axis

The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs). These components affect immune function, brain development and behaviour...

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Autores principales: Szklany, Kirsten, Engen, Phillip A., Naqib, Ankur, Green, Stefan J., Keshavarzian, Ali, Lopez Rincon, Alejandro, Siebrand, Cynthia J., Diks, Mara A. P., van de Kaa, Melanie, Garssen, Johan, Knippels, Leon M. J., Kraneveld, Aletta D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746884/
https://www.ncbi.nlm.nih.gov/pubmed/35011046
http://dx.doi.org/10.3390/nu14010173
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author Szklany, Kirsten
Engen, Phillip A.
Naqib, Ankur
Green, Stefan J.
Keshavarzian, Ali
Lopez Rincon, Alejandro
Siebrand, Cynthia J.
Diks, Mara A. P.
van de Kaa, Melanie
Garssen, Johan
Knippels, Leon M. J.
Kraneveld, Aletta D.
author_facet Szklany, Kirsten
Engen, Phillip A.
Naqib, Ankur
Green, Stefan J.
Keshavarzian, Ali
Lopez Rincon, Alejandro
Siebrand, Cynthia J.
Diks, Mara A. P.
van de Kaa, Melanie
Garssen, Johan
Knippels, Leon M. J.
Kraneveld, Aletta D.
author_sort Szklany, Kirsten
collection PubMed
description The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs). These components affect immune function, brain development and behaviour. We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice. Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs). Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed. Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups. Compared to control diet, the scGOS:lcFOS and combination diets increased caecal saccharolytic fermentation activity. The diets enhanced the number of enterochromaffin cells. The combination diet had no effects on the immune cells. Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group. The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system. No apparent additive effect was observed when scGOS:lcFOS and n-3 PUFAs were combined. The results suggest that scGOS:lcFOS and n-3 PUFAs together create a balance—the best of both in a healthy host.
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spelling pubmed-87468842022-01-11 Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut–Immune System–Brain Axis Szklany, Kirsten Engen, Phillip A. Naqib, Ankur Green, Stefan J. Keshavarzian, Ali Lopez Rincon, Alejandro Siebrand, Cynthia J. Diks, Mara A. P. van de Kaa, Melanie Garssen, Johan Knippels, Leon M. J. Kraneveld, Aletta D. Nutrients Article The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs). These components affect immune function, brain development and behaviour. We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice. Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs). Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed. Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups. Compared to control diet, the scGOS:lcFOS and combination diets increased caecal saccharolytic fermentation activity. The diets enhanced the number of enterochromaffin cells. The combination diet had no effects on the immune cells. Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group. The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system. No apparent additive effect was observed when scGOS:lcFOS and n-3 PUFAs were combined. The results suggest that scGOS:lcFOS and n-3 PUFAs together create a balance—the best of both in a healthy host. MDPI 2021-12-30 /pmc/articles/PMC8746884/ /pubmed/35011046 http://dx.doi.org/10.3390/nu14010173 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szklany, Kirsten
Engen, Phillip A.
Naqib, Ankur
Green, Stefan J.
Keshavarzian, Ali
Lopez Rincon, Alejandro
Siebrand, Cynthia J.
Diks, Mara A. P.
van de Kaa, Melanie
Garssen, Johan
Knippels, Leon M. J.
Kraneveld, Aletta D.
Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut–Immune System–Brain Axis
title Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut–Immune System–Brain Axis
title_full Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut–Immune System–Brain Axis
title_fullStr Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut–Immune System–Brain Axis
title_full_unstemmed Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut–Immune System–Brain Axis
title_short Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut–Immune System–Brain Axis
title_sort dietary supplementation throughout life with non-digestible oligosaccharides and/or n-3 poly-unsaturated fatty acids in healthy mice modulates the gut–immune system–brain axis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8746884/
https://www.ncbi.nlm.nih.gov/pubmed/35011046
http://dx.doi.org/10.3390/nu14010173
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