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Changes in Triterpenes in Alismatis rhizoma after Processing Based on Targeted Metabolomics Using UHPLC-QTOF-MS/MS

Alismatis rhizoma (AR) has been used as an herbal medicine in China for over a thousand years. Crude AR, salt-processed AR (SAR), and bran-processed AR (BAR) are recorded in the Pharmacopoeia of the People′s Republic of China. However, the differences of chemical composition between crude AR and its...

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Autores principales: Dai, Mengxiang, Li, Sen, Shi, Qingxin, Xiang, Xingliang, Jin, Yuehui, Wei, Sha, Zhang, Lijun, Yang, Min, Song, Chengwu, Huang, Rongzeng, Jin, Shuna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747029/
https://www.ncbi.nlm.nih.gov/pubmed/35011422
http://dx.doi.org/10.3390/molecules27010185
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author Dai, Mengxiang
Li, Sen
Shi, Qingxin
Xiang, Xingliang
Jin, Yuehui
Wei, Sha
Zhang, Lijun
Yang, Min
Song, Chengwu
Huang, Rongzeng
Jin, Shuna
author_facet Dai, Mengxiang
Li, Sen
Shi, Qingxin
Xiang, Xingliang
Jin, Yuehui
Wei, Sha
Zhang, Lijun
Yang, Min
Song, Chengwu
Huang, Rongzeng
Jin, Shuna
author_sort Dai, Mengxiang
collection PubMed
description Alismatis rhizoma (AR) has been used as an herbal medicine in China for over a thousand years. Crude AR, salt-processed AR (SAR), and bran-processed AR (BAR) are recorded in the Pharmacopoeia of the People′s Republic of China. However, the differences of chemical composition between crude AR and its processing products remain limited. In this study, triterpenes were identified from crude AR, SAR, and BAR by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight-mass spectrometer (UHPLC-QTOF-MS/MS). Subsequently, the differences of triterpenes between the crude AR and processed ARs were compared via a targeted metabolomics approach. Finally, a total of 114 triterpenes were identified, of which 83, 100, and 103 triterpenes were found in crude AR, SAR, and BAR, respectively. After salt-processing, there were 17 triterpenes newly generated, 7 triterpenes with trends of increasing, and 37 triterpenes decreased. Meanwhile, 56 triterpenes including 21 newly generated and 35 with significant increases were observed in BAR. This study could be benefit to investigate the processing mechanism of AR, as well as support their clinical applications.
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spelling pubmed-87470292022-01-11 Changes in Triterpenes in Alismatis rhizoma after Processing Based on Targeted Metabolomics Using UHPLC-QTOF-MS/MS Dai, Mengxiang Li, Sen Shi, Qingxin Xiang, Xingliang Jin, Yuehui Wei, Sha Zhang, Lijun Yang, Min Song, Chengwu Huang, Rongzeng Jin, Shuna Molecules Article Alismatis rhizoma (AR) has been used as an herbal medicine in China for over a thousand years. Crude AR, salt-processed AR (SAR), and bran-processed AR (BAR) are recorded in the Pharmacopoeia of the People′s Republic of China. However, the differences of chemical composition between crude AR and its processing products remain limited. In this study, triterpenes were identified from crude AR, SAR, and BAR by ultra-high performance liquid chromatography coupled with quadrupole time-of-flight-mass spectrometer (UHPLC-QTOF-MS/MS). Subsequently, the differences of triterpenes between the crude AR and processed ARs were compared via a targeted metabolomics approach. Finally, a total of 114 triterpenes were identified, of which 83, 100, and 103 triterpenes were found in crude AR, SAR, and BAR, respectively. After salt-processing, there were 17 triterpenes newly generated, 7 triterpenes with trends of increasing, and 37 triterpenes decreased. Meanwhile, 56 triterpenes including 21 newly generated and 35 with significant increases were observed in BAR. This study could be benefit to investigate the processing mechanism of AR, as well as support their clinical applications. MDPI 2021-12-29 /pmc/articles/PMC8747029/ /pubmed/35011422 http://dx.doi.org/10.3390/molecules27010185 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dai, Mengxiang
Li, Sen
Shi, Qingxin
Xiang, Xingliang
Jin, Yuehui
Wei, Sha
Zhang, Lijun
Yang, Min
Song, Chengwu
Huang, Rongzeng
Jin, Shuna
Changes in Triterpenes in Alismatis rhizoma after Processing Based on Targeted Metabolomics Using UHPLC-QTOF-MS/MS
title Changes in Triterpenes in Alismatis rhizoma after Processing Based on Targeted Metabolomics Using UHPLC-QTOF-MS/MS
title_full Changes in Triterpenes in Alismatis rhizoma after Processing Based on Targeted Metabolomics Using UHPLC-QTOF-MS/MS
title_fullStr Changes in Triterpenes in Alismatis rhizoma after Processing Based on Targeted Metabolomics Using UHPLC-QTOF-MS/MS
title_full_unstemmed Changes in Triterpenes in Alismatis rhizoma after Processing Based on Targeted Metabolomics Using UHPLC-QTOF-MS/MS
title_short Changes in Triterpenes in Alismatis rhizoma after Processing Based on Targeted Metabolomics Using UHPLC-QTOF-MS/MS
title_sort changes in triterpenes in alismatis rhizoma after processing based on targeted metabolomics using uhplc-qtof-ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747029/
https://www.ncbi.nlm.nih.gov/pubmed/35011422
http://dx.doi.org/10.3390/molecules27010185
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