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Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity

Obesity rates among children are growing rapidly worldwide, placing massive pressure on healthcare systems. Untargeted metabolomics can expand our understanding of the pathogenesis of obesity and elucidate mechanisms related to its symptoms. However, the metabolic signatures of obesity in children h...

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Autores principales: Szczerbinski, Lukasz, Wojciechowska, Gladys, Olichwier, Adam, Taylor, Mark A., Puchta, Urszula, Konopka, Paulina, Paszko, Adam, Citko, Anna, Goscik, Joanna, Fiehn, Oliver, Fan, Sili, Wasilewska, Anna, Taranta-Janusz, Katarzyna, Kretowski, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747180/
https://www.ncbi.nlm.nih.gov/pubmed/35011090
http://dx.doi.org/10.3390/nu14010214
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author Szczerbinski, Lukasz
Wojciechowska, Gladys
Olichwier, Adam
Taylor, Mark A.
Puchta, Urszula
Konopka, Paulina
Paszko, Adam
Citko, Anna
Goscik, Joanna
Fiehn, Oliver
Fan, Sili
Wasilewska, Anna
Taranta-Janusz, Katarzyna
Kretowski, Adam
author_facet Szczerbinski, Lukasz
Wojciechowska, Gladys
Olichwier, Adam
Taylor, Mark A.
Puchta, Urszula
Konopka, Paulina
Paszko, Adam
Citko, Anna
Goscik, Joanna
Fiehn, Oliver
Fan, Sili
Wasilewska, Anna
Taranta-Janusz, Katarzyna
Kretowski, Adam
author_sort Szczerbinski, Lukasz
collection PubMed
description Obesity rates among children are growing rapidly worldwide, placing massive pressure on healthcare systems. Untargeted metabolomics can expand our understanding of the pathogenesis of obesity and elucidate mechanisms related to its symptoms. However, the metabolic signatures of obesity in children have not been thoroughly investigated. Herein, we explored metabolites associated with obesity development in childhood. Untargeted metabolomic profiling was performed on fasting serum samples from 27 obese Caucasian children and adolescents and 15 sex- and age-matched normal-weight children. Three metabolomic assays were combined and yielded 726 unique identified metabolites: gas chromatography–mass spectrometry (GC–MS), hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC LC–MS/MS), and lipidomics. Univariate and multivariate analyses showed clear discrimination between the untargeted metabolomes of obese and normal-weight children, with 162 significantly differentially expressed metabolites between groups. Children with obesity had higher concentrations of branch-chained amino acids and various lipid metabolites, including phosphatidylcholines, cholesteryl esters, triglycerides. Thus, an early manifestation of obesity pathogenesis and its metabolic consequences in the serum metabolome are correlated with altered lipid metabolism. Obesity metabolite patterns in the adult population were very similar to the metabolic signature of childhood obesity. Identified metabolites could be potential biomarkers and used to study obesity pathomechanisms.
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spelling pubmed-87471802022-01-11 Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity Szczerbinski, Lukasz Wojciechowska, Gladys Olichwier, Adam Taylor, Mark A. Puchta, Urszula Konopka, Paulina Paszko, Adam Citko, Anna Goscik, Joanna Fiehn, Oliver Fan, Sili Wasilewska, Anna Taranta-Janusz, Katarzyna Kretowski, Adam Nutrients Article Obesity rates among children are growing rapidly worldwide, placing massive pressure on healthcare systems. Untargeted metabolomics can expand our understanding of the pathogenesis of obesity and elucidate mechanisms related to its symptoms. However, the metabolic signatures of obesity in children have not been thoroughly investigated. Herein, we explored metabolites associated with obesity development in childhood. Untargeted metabolomic profiling was performed on fasting serum samples from 27 obese Caucasian children and adolescents and 15 sex- and age-matched normal-weight children. Three metabolomic assays were combined and yielded 726 unique identified metabolites: gas chromatography–mass spectrometry (GC–MS), hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC LC–MS/MS), and lipidomics. Univariate and multivariate analyses showed clear discrimination between the untargeted metabolomes of obese and normal-weight children, with 162 significantly differentially expressed metabolites between groups. Children with obesity had higher concentrations of branch-chained amino acids and various lipid metabolites, including phosphatidylcholines, cholesteryl esters, triglycerides. Thus, an early manifestation of obesity pathogenesis and its metabolic consequences in the serum metabolome are correlated with altered lipid metabolism. Obesity metabolite patterns in the adult population were very similar to the metabolic signature of childhood obesity. Identified metabolites could be potential biomarkers and used to study obesity pathomechanisms. MDPI 2022-01-04 /pmc/articles/PMC8747180/ /pubmed/35011090 http://dx.doi.org/10.3390/nu14010214 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szczerbinski, Lukasz
Wojciechowska, Gladys
Olichwier, Adam
Taylor, Mark A.
Puchta, Urszula
Konopka, Paulina
Paszko, Adam
Citko, Anna
Goscik, Joanna
Fiehn, Oliver
Fan, Sili
Wasilewska, Anna
Taranta-Janusz, Katarzyna
Kretowski, Adam
Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity
title Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity
title_full Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity
title_fullStr Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity
title_full_unstemmed Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity
title_short Untargeted Metabolomics Analysis of the Serum Metabolic Signature of Childhood Obesity
title_sort untargeted metabolomics analysis of the serum metabolic signature of childhood obesity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747180/
https://www.ncbi.nlm.nih.gov/pubmed/35011090
http://dx.doi.org/10.3390/nu14010214
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