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Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells
Background: Dyslipidaemias, particularly elevated plasma low-density lipoprotein cholesterol (LDL-C) levels, are major risk factors for cardiovascular disease (CVD). Besides pharmacological approaches, a nutritional strategy for CVD prevention has gained increasing attention. Among functional foods,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747205/ https://www.ncbi.nlm.nih.gov/pubmed/35011066 http://dx.doi.org/10.3390/nu14010193 |
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author | Macchi, Chiara Greco, Maria Francesca Ferri, Nicola Magni, Paolo Arnoldi, Anna Corsini, Alberto Sirtori, Cesare R. Ruscica, Massimiliano Lammi, Carmen |
author_facet | Macchi, Chiara Greco, Maria Francesca Ferri, Nicola Magni, Paolo Arnoldi, Anna Corsini, Alberto Sirtori, Cesare R. Ruscica, Massimiliano Lammi, Carmen |
author_sort | Macchi, Chiara |
collection | PubMed |
description | Background: Dyslipidaemias, particularly elevated plasma low-density lipoprotein cholesterol (LDL-C) levels, are major risk factors for cardiovascular disease (CVD). Besides pharmacological approaches, a nutritional strategy for CVD prevention has gained increasing attention. Among functional foods, the hypocholesterolemic properties of soy are driven by a stimulation of LDL-receptor (LDL-R) activity. Aim: To characterize the effect of two soy peptides, namely, β-conglycinin-derived YVVNPDNDEN and YVVNPDNNEN on the expression of proprotein convertase subtilisin/kexin type 9 (PCSK9), one of the key-regulators of the LDL-R. Methods: PCSK9 promoter activity (luciferase assay), PCSK9 protein expression (WB) and secretion (ELISA), PCSK9 interaction with LDL-R (binding assay) and human HepG2 cells were the objects of this investigation. Results: Treatment with YVVNPDNNEN peptide has led to a rise in PCSK9 gene expression (90.8%) and transcriptional activity (86.4%), and to a decrement in PCSK9 intracellular and secreted protein (−42.9%) levels. YVVNPDNNEN peptide reduced the protein expression of transcriptional factor HNF1α. Most changes driven by YVVNPDNDEN peptide were not statistically significant. Neither peptide inhibited the PCSK9–LDLR interaction. Conclusions: Although sharing a common effect on LDL-R levels through the inhibition of 3-hydroxy-3-methylglutaryl CoA reductase activity, only the YVVNPDNNEN peptide has an additional mechanism via the downregulation of PCSK9 protein levels. |
format | Online Article Text |
id | pubmed-8747205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87472052022-01-11 Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells Macchi, Chiara Greco, Maria Francesca Ferri, Nicola Magni, Paolo Arnoldi, Anna Corsini, Alberto Sirtori, Cesare R. Ruscica, Massimiliano Lammi, Carmen Nutrients Article Background: Dyslipidaemias, particularly elevated plasma low-density lipoprotein cholesterol (LDL-C) levels, are major risk factors for cardiovascular disease (CVD). Besides pharmacological approaches, a nutritional strategy for CVD prevention has gained increasing attention. Among functional foods, the hypocholesterolemic properties of soy are driven by a stimulation of LDL-receptor (LDL-R) activity. Aim: To characterize the effect of two soy peptides, namely, β-conglycinin-derived YVVNPDNDEN and YVVNPDNNEN on the expression of proprotein convertase subtilisin/kexin type 9 (PCSK9), one of the key-regulators of the LDL-R. Methods: PCSK9 promoter activity (luciferase assay), PCSK9 protein expression (WB) and secretion (ELISA), PCSK9 interaction with LDL-R (binding assay) and human HepG2 cells were the objects of this investigation. Results: Treatment with YVVNPDNNEN peptide has led to a rise in PCSK9 gene expression (90.8%) and transcriptional activity (86.4%), and to a decrement in PCSK9 intracellular and secreted protein (−42.9%) levels. YVVNPDNNEN peptide reduced the protein expression of transcriptional factor HNF1α. Most changes driven by YVVNPDNDEN peptide were not statistically significant. Neither peptide inhibited the PCSK9–LDLR interaction. Conclusions: Although sharing a common effect on LDL-R levels through the inhibition of 3-hydroxy-3-methylglutaryl CoA reductase activity, only the YVVNPDNNEN peptide has an additional mechanism via the downregulation of PCSK9 protein levels. MDPI 2021-12-31 /pmc/articles/PMC8747205/ /pubmed/35011066 http://dx.doi.org/10.3390/nu14010193 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Macchi, Chiara Greco, Maria Francesca Ferri, Nicola Magni, Paolo Arnoldi, Anna Corsini, Alberto Sirtori, Cesare R. Ruscica, Massimiliano Lammi, Carmen Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells |
title | Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells |
title_full | Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells |
title_fullStr | Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells |
title_full_unstemmed | Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells |
title_short | Impact of Soy β-Conglycinin Peptides on PCSK9 Protein Expression in HepG2 Cells |
title_sort | impact of soy β-conglycinin peptides on pcsk9 protein expression in hepg2 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747205/ https://www.ncbi.nlm.nih.gov/pubmed/35011066 http://dx.doi.org/10.3390/nu14010193 |
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