Cargando…

Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits

The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial, antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Brčić Karačonji, Irena, Jurica, Karlo, Gašić, Uroš, Dramićanin, Aleksandra, Tešić, Živoslav, Milojković Opsenica, Dušanka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747429/
https://www.ncbi.nlm.nih.gov/pubmed/35009028
http://dx.doi.org/10.3390/plants11010025
_version_ 1784630834399019008
author Brčić Karačonji, Irena
Jurica, Karlo
Gašić, Uroš
Dramićanin, Aleksandra
Tešić, Živoslav
Milojković Opsenica, Dušanka
author_facet Brčić Karačonji, Irena
Jurica, Karlo
Gašić, Uroš
Dramićanin, Aleksandra
Tešić, Živoslav
Milojković Opsenica, Dušanka
author_sort Brčić Karačonji, Irena
collection PubMed
description The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial, antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was determined by Folin–Ciocalteu’s assay, while RSA was investigated using DPPH reagent. A total of 64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were the major compounds found in fruits. To the authors’ knowledge, 16 and 5 phenolics in leaves and fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of 67.07–104.74 and 16.78–25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA for leaves and fruits were in the ranges of 408.92–430.98 and 74.30–104.04 μmol Trolox equivalents (TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves. Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to A. unedo as a promising health-promoting plant and natural food preservative.
format Online
Article
Text
id pubmed-8747429
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87474292022-01-11 Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits Brčić Karačonji, Irena Jurica, Karlo Gašić, Uroš Dramićanin, Aleksandra Tešić, Živoslav Milojković Opsenica, Dušanka Plants (Basel) Article The strawberry tree (Arbutus unedo L., Ericaceae family) is an evergreen Mediterranean shrub whose leaves and fruits are used in traditional medicine due to their antioxidant, antimicrobial, antidiabetic, diuretic, and antiproliferative properties. The health benefits are mainly attributed to the presence of phenolic compounds. The aim of this study was to compare the phenolic profiles, total phenolic content (TPC), and radical scavenging activity (RSA) of A. unedo leaves and fruits collected at two locations in Croatia. Phenolic profiles were identified using an ultra-high-performance liquid chromatograph (UHPLC) coupled with a hybrid mass spectrometer (LTQ Orbitrap MS). TPC was determined by Folin–Ciocalteu’s assay, while RSA was investigated using DPPH reagent. A total of 64 phenolics (60 and 42 compounds in leaves and fruits, respectively) were identified. Hyperoside and flavan-3-ols were predominant compounds in leaves, while gallocatechin and catechin were the major compounds found in fruits. To the authors’ knowledge, 16 and 5 phenolics in leaves and fruits, respectively, were reported for the first time. Principal component analysis (PCA) showed that UHPLC-LTQ Orbitrap MS could be used to identify which phenolics were able to discriminate samples regarding plant tissue and geographical origin. TPC in leaves and fruits were in the ranges of 67.07–104.74 and 16.78–25.86 mg gallic acid equivalents (GAE)/g dried weight (dw), respectively. RSA for leaves and fruits were in the ranges of 408.92–430.98 and 74.30–104.04 μmol Trolox equivalents (TE)/g dw, respectively. The number of identified phenolics was lower in fruits compared to leaves. Such a large number of bioactive phenolics identified and the strong antioxidant activity pointed to A. unedo as a promising health-promoting plant and natural food preservative. MDPI 2021-12-22 /pmc/articles/PMC8747429/ /pubmed/35009028 http://dx.doi.org/10.3390/plants11010025 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brčić Karačonji, Irena
Jurica, Karlo
Gašić, Uroš
Dramićanin, Aleksandra
Tešić, Živoslav
Milojković Opsenica, Dušanka
Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
title Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
title_full Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
title_fullStr Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
title_full_unstemmed Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
title_short Comparative Study on the Phenolic Fingerprint and Antioxidant Activity of Strawberry Tree (Arbutus unedo L.) Leaves and Fruits
title_sort comparative study on the phenolic fingerprint and antioxidant activity of strawberry tree (arbutus unedo l.) leaves and fruits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747429/
https://www.ncbi.nlm.nih.gov/pubmed/35009028
http://dx.doi.org/10.3390/plants11010025
work_keys_str_mv AT brcickaraconjiirena comparativestudyonthephenolicfingerprintandantioxidantactivityofstrawberrytreearbutusunedolleavesandfruits
AT juricakarlo comparativestudyonthephenolicfingerprintandantioxidantactivityofstrawberrytreearbutusunedolleavesandfruits
AT gasicuros comparativestudyonthephenolicfingerprintandantioxidantactivityofstrawberrytreearbutusunedolleavesandfruits
AT dramicaninaleksandra comparativestudyonthephenolicfingerprintandantioxidantactivityofstrawberrytreearbutusunedolleavesandfruits
AT tesiczivoslav comparativestudyonthephenolicfingerprintandantioxidantactivityofstrawberrytreearbutusunedolleavesandfruits
AT milojkovicopsenicadusanka comparativestudyonthephenolicfingerprintandantioxidantactivityofstrawberrytreearbutusunedolleavesandfruits