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The Elaboration of Effective Coatings for Photonic Crystal Chips in Optical Biosensors
Here, we propose and study several types of quartz surface coatings designed for the high-performance sorption of biomolecules and their subsequent detection by a photonic crystal surface mode (PC SM) biosensor. The deposition and sorption of biomolecules are revealed by analyzing changes in the pro...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747551/ https://www.ncbi.nlm.nih.gov/pubmed/35012173 http://dx.doi.org/10.3390/polym14010152 |
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author | Sizova, Svetlana Shakurov, Ruslan Mitko, Tatiana Shirshikov, Fedor Solovyeva, Daria Konopsky, Valery Alieva, Elena Klinov, Dmitry Bespyatykh, Julia Basmanov, Dmitry |
author_facet | Sizova, Svetlana Shakurov, Ruslan Mitko, Tatiana Shirshikov, Fedor Solovyeva, Daria Konopsky, Valery Alieva, Elena Klinov, Dmitry Bespyatykh, Julia Basmanov, Dmitry |
author_sort | Sizova, Svetlana |
collection | PubMed |
description | Here, we propose and study several types of quartz surface coatings designed for the high-performance sorption of biomolecules and their subsequent detection by a photonic crystal surface mode (PC SM) biosensor. The deposition and sorption of biomolecules are revealed by analyzing changes in the propagation parameters of optical modes on the surface of a photonic crystal (PC). The method makes it possible to measure molecular and cellular affinity interactions in real time by independently recording the values of the angle of total internal reflection and the angle of excitation of the surface wave on the surface of the PC. A series of dextrans with various anchor groups (aldehyde, carboxy, epoxy) suitable for binding with bioligands have been studied. We have carried out comparative experiments with dextrans with other molecular weights. The results confirmed that dextran with a Mw of 500 kDa and anchor epoxy groups have a promising potential as a matrix for the detection of proteins in optical biosensors. The proposed approach would make it possible to enhance the sensitivity of the PC SM biosensor and also permit studying the binding process of low molecular weight molecules in real time. |
format | Online Article Text |
id | pubmed-8747551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87475512022-01-11 The Elaboration of Effective Coatings for Photonic Crystal Chips in Optical Biosensors Sizova, Svetlana Shakurov, Ruslan Mitko, Tatiana Shirshikov, Fedor Solovyeva, Daria Konopsky, Valery Alieva, Elena Klinov, Dmitry Bespyatykh, Julia Basmanov, Dmitry Polymers (Basel) Article Here, we propose and study several types of quartz surface coatings designed for the high-performance sorption of biomolecules and their subsequent detection by a photonic crystal surface mode (PC SM) biosensor. The deposition and sorption of biomolecules are revealed by analyzing changes in the propagation parameters of optical modes on the surface of a photonic crystal (PC). The method makes it possible to measure molecular and cellular affinity interactions in real time by independently recording the values of the angle of total internal reflection and the angle of excitation of the surface wave on the surface of the PC. A series of dextrans with various anchor groups (aldehyde, carboxy, epoxy) suitable for binding with bioligands have been studied. We have carried out comparative experiments with dextrans with other molecular weights. The results confirmed that dextran with a Mw of 500 kDa and anchor epoxy groups have a promising potential as a matrix for the detection of proteins in optical biosensors. The proposed approach would make it possible to enhance the sensitivity of the PC SM biosensor and also permit studying the binding process of low molecular weight molecules in real time. MDPI 2021-12-31 /pmc/articles/PMC8747551/ /pubmed/35012173 http://dx.doi.org/10.3390/polym14010152 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sizova, Svetlana Shakurov, Ruslan Mitko, Tatiana Shirshikov, Fedor Solovyeva, Daria Konopsky, Valery Alieva, Elena Klinov, Dmitry Bespyatykh, Julia Basmanov, Dmitry The Elaboration of Effective Coatings for Photonic Crystal Chips in Optical Biosensors |
title | The Elaboration of Effective Coatings for Photonic Crystal Chips in Optical Biosensors |
title_full | The Elaboration of Effective Coatings for Photonic Crystal Chips in Optical Biosensors |
title_fullStr | The Elaboration of Effective Coatings for Photonic Crystal Chips in Optical Biosensors |
title_full_unstemmed | The Elaboration of Effective Coatings for Photonic Crystal Chips in Optical Biosensors |
title_short | The Elaboration of Effective Coatings for Photonic Crystal Chips in Optical Biosensors |
title_sort | elaboration of effective coatings for photonic crystal chips in optical biosensors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747551/ https://www.ncbi.nlm.nih.gov/pubmed/35012173 http://dx.doi.org/10.3390/polym14010152 |
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