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Optimization of Potassium Supply under Osmotic Stress Mitigates Oxidative Damage in Barley

Potassium (K) is the most abundant cation in plants, playing an important role in osmoregulation. Little is known about the effect of genotypic variation in the tolerance to osmotic stress under different K treatments in barley. In this study, we measured the interactive effects of osmotic stress an...

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Autores principales: Tavakol, Ershad, Jákli, Bálint, Cakmak, Ismail, Dittert, Klaus, Senbayram, Mehmet
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747552/
https://www.ncbi.nlm.nih.gov/pubmed/35009058
http://dx.doi.org/10.3390/plants11010055
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author Tavakol, Ershad
Jákli, Bálint
Cakmak, Ismail
Dittert, Klaus
Senbayram, Mehmet
author_facet Tavakol, Ershad
Jákli, Bálint
Cakmak, Ismail
Dittert, Klaus
Senbayram, Mehmet
author_sort Tavakol, Ershad
collection PubMed
description Potassium (K) is the most abundant cation in plants, playing an important role in osmoregulation. Little is known about the effect of genotypic variation in the tolerance to osmotic stress under different K treatments in barley. In this study, we measured the interactive effects of osmotic stress and K supply on growth and stress responses of two barley cultivars (Hordeum vulgare L.) and monitored reactive oxygen species (ROS) along with enzymatic antioxidant activity and their respective gene expression level. The selected cultivars (cv. Milford and cv. Sahin-91Sahin-91) were exposed to osmotic stress (−0.7 MPa) induced by polyethylene glycol 6000 (PEG) under low (0.04 mM) and adequate (0.8 mM) K levels in the nutrient solution. Leaf samples were collected and analyzed for levels of K, ROS, kinetic activity of antioxidants enzymes and expression levels of respective genes during the stress period. The results showed that optimal K supply under osmotic stress significantly decreases ROS production and adjusts antioxidant activity, leading to the reduction of oxidative stress in the studied plants. The cultivar Milford had a lower ROS level and a better tolerance to stress compared to the cultivar Sahin-91. We conclude that optimized K supply is of great importance in mitigating ROS-related damage induced by osmotic stress, specifically in drought-sensitive barley cultivars.
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spelling pubmed-87475522022-01-11 Optimization of Potassium Supply under Osmotic Stress Mitigates Oxidative Damage in Barley Tavakol, Ershad Jákli, Bálint Cakmak, Ismail Dittert, Klaus Senbayram, Mehmet Plants (Basel) Article Potassium (K) is the most abundant cation in plants, playing an important role in osmoregulation. Little is known about the effect of genotypic variation in the tolerance to osmotic stress under different K treatments in barley. In this study, we measured the interactive effects of osmotic stress and K supply on growth and stress responses of two barley cultivars (Hordeum vulgare L.) and monitored reactive oxygen species (ROS) along with enzymatic antioxidant activity and their respective gene expression level. The selected cultivars (cv. Milford and cv. Sahin-91Sahin-91) were exposed to osmotic stress (−0.7 MPa) induced by polyethylene glycol 6000 (PEG) under low (0.04 mM) and adequate (0.8 mM) K levels in the nutrient solution. Leaf samples were collected and analyzed for levels of K, ROS, kinetic activity of antioxidants enzymes and expression levels of respective genes during the stress period. The results showed that optimal K supply under osmotic stress significantly decreases ROS production and adjusts antioxidant activity, leading to the reduction of oxidative stress in the studied plants. The cultivar Milford had a lower ROS level and a better tolerance to stress compared to the cultivar Sahin-91. We conclude that optimized K supply is of great importance in mitigating ROS-related damage induced by osmotic stress, specifically in drought-sensitive barley cultivars. MDPI 2021-12-25 /pmc/articles/PMC8747552/ /pubmed/35009058 http://dx.doi.org/10.3390/plants11010055 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tavakol, Ershad
Jákli, Bálint
Cakmak, Ismail
Dittert, Klaus
Senbayram, Mehmet
Optimization of Potassium Supply under Osmotic Stress Mitigates Oxidative Damage in Barley
title Optimization of Potassium Supply under Osmotic Stress Mitigates Oxidative Damage in Barley
title_full Optimization of Potassium Supply under Osmotic Stress Mitigates Oxidative Damage in Barley
title_fullStr Optimization of Potassium Supply under Osmotic Stress Mitigates Oxidative Damage in Barley
title_full_unstemmed Optimization of Potassium Supply under Osmotic Stress Mitigates Oxidative Damage in Barley
title_short Optimization of Potassium Supply under Osmotic Stress Mitigates Oxidative Damage in Barley
title_sort optimization of potassium supply under osmotic stress mitigates oxidative damage in barley
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747552/
https://www.ncbi.nlm.nih.gov/pubmed/35009058
http://dx.doi.org/10.3390/plants11010055
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