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Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles

Novel molecularly imprinted polymers (MIPs) represent a selectively recognized technique for electrochemical detection design. This rapid and simple method prepared via chemical synthesis consists of a monomer crosslinked with an initiator, whereas low sensitivity remains a drawback. Nanomaterials c...

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Autores principales: Limthin, Dalawan, Leepheng, Piyawan, Klamchuen, Annop, Phromyothin, Darinee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747701/
https://www.ncbi.nlm.nih.gov/pubmed/35012114
http://dx.doi.org/10.3390/polym14010091
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author Limthin, Dalawan
Leepheng, Piyawan
Klamchuen, Annop
Phromyothin, Darinee
author_facet Limthin, Dalawan
Leepheng, Piyawan
Klamchuen, Annop
Phromyothin, Darinee
author_sort Limthin, Dalawan
collection PubMed
description Novel molecularly imprinted polymers (MIPs) represent a selectively recognized technique for electrochemical detection design. This rapid and simple method prepared via chemical synthesis consists of a monomer crosslinked with an initiator, whereas low sensitivity remains a drawback. Nanomaterials can improve charge transfer for MIP surface modification in order to overcome this problem. SPIONs have semiconductor and superparamagnetic properties that can enhance carrier mobility, causing high sensitivity of electrochemical detection. In this work, surface modification was achieved with a combination of MIP and SPIONs for gluten detection. The SPIONs were synthesized via the chemical co-precipitation method and mixed with MIPs by polymerizing gluten and methyl methacrylate (MMA), presented as a template and a monomer. Magnetic MIP (MMIP) was modified on a carbon-plate electrode. The morphology of modified electrode surfaces was determined by scanning electron microscopy–energy-dispersive X-ray spectrometry. The performance of the MMIP electrode was confirmed by cyclic voltammetry, amperometry, and electrochemical impedance spectroscopy. The MMIP electrode for gluten detection shows a dynamic linear range of 5–50 ppm, with a correlation coefficient of 0.994 and a low detection limit of 1.50 ppm, which is less than the U.S. Food and Drug Administration requirements (20 ppm); moreover, it exhibits excellent selectivity, sensitivity, stability, and reproducibility.
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spelling pubmed-87477012022-01-11 Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles Limthin, Dalawan Leepheng, Piyawan Klamchuen, Annop Phromyothin, Darinee Polymers (Basel) Article Novel molecularly imprinted polymers (MIPs) represent a selectively recognized technique for electrochemical detection design. This rapid and simple method prepared via chemical synthesis consists of a monomer crosslinked with an initiator, whereas low sensitivity remains a drawback. Nanomaterials can improve charge transfer for MIP surface modification in order to overcome this problem. SPIONs have semiconductor and superparamagnetic properties that can enhance carrier mobility, causing high sensitivity of electrochemical detection. In this work, surface modification was achieved with a combination of MIP and SPIONs for gluten detection. The SPIONs were synthesized via the chemical co-precipitation method and mixed with MIPs by polymerizing gluten and methyl methacrylate (MMA), presented as a template and a monomer. Magnetic MIP (MMIP) was modified on a carbon-plate electrode. The morphology of modified electrode surfaces was determined by scanning electron microscopy–energy-dispersive X-ray spectrometry. The performance of the MMIP electrode was confirmed by cyclic voltammetry, amperometry, and electrochemical impedance spectroscopy. The MMIP electrode for gluten detection shows a dynamic linear range of 5–50 ppm, with a correlation coefficient of 0.994 and a low detection limit of 1.50 ppm, which is less than the U.S. Food and Drug Administration requirements (20 ppm); moreover, it exhibits excellent selectivity, sensitivity, stability, and reproducibility. MDPI 2021-12-27 /pmc/articles/PMC8747701/ /pubmed/35012114 http://dx.doi.org/10.3390/polym14010091 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Limthin, Dalawan
Leepheng, Piyawan
Klamchuen, Annop
Phromyothin, Darinee
Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles
title Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles
title_full Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles
title_fullStr Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles
title_full_unstemmed Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles
title_short Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles
title_sort enhancement of electrochemical detection of gluten with surface modification based on molecularly imprinted polymers combined with superparamagnetic iron oxide nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747701/
https://www.ncbi.nlm.nih.gov/pubmed/35012114
http://dx.doi.org/10.3390/polym14010091
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