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Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles
Novel molecularly imprinted polymers (MIPs) represent a selectively recognized technique for electrochemical detection design. This rapid and simple method prepared via chemical synthesis consists of a monomer crosslinked with an initiator, whereas low sensitivity remains a drawback. Nanomaterials c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747701/ https://www.ncbi.nlm.nih.gov/pubmed/35012114 http://dx.doi.org/10.3390/polym14010091 |
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author | Limthin, Dalawan Leepheng, Piyawan Klamchuen, Annop Phromyothin, Darinee |
author_facet | Limthin, Dalawan Leepheng, Piyawan Klamchuen, Annop Phromyothin, Darinee |
author_sort | Limthin, Dalawan |
collection | PubMed |
description | Novel molecularly imprinted polymers (MIPs) represent a selectively recognized technique for electrochemical detection design. This rapid and simple method prepared via chemical synthesis consists of a monomer crosslinked with an initiator, whereas low sensitivity remains a drawback. Nanomaterials can improve charge transfer for MIP surface modification in order to overcome this problem. SPIONs have semiconductor and superparamagnetic properties that can enhance carrier mobility, causing high sensitivity of electrochemical detection. In this work, surface modification was achieved with a combination of MIP and SPIONs for gluten detection. The SPIONs were synthesized via the chemical co-precipitation method and mixed with MIPs by polymerizing gluten and methyl methacrylate (MMA), presented as a template and a monomer. Magnetic MIP (MMIP) was modified on a carbon-plate electrode. The morphology of modified electrode surfaces was determined by scanning electron microscopy–energy-dispersive X-ray spectrometry. The performance of the MMIP electrode was confirmed by cyclic voltammetry, amperometry, and electrochemical impedance spectroscopy. The MMIP electrode for gluten detection shows a dynamic linear range of 5–50 ppm, with a correlation coefficient of 0.994 and a low detection limit of 1.50 ppm, which is less than the U.S. Food and Drug Administration requirements (20 ppm); moreover, it exhibits excellent selectivity, sensitivity, stability, and reproducibility. |
format | Online Article Text |
id | pubmed-8747701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87477012022-01-11 Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles Limthin, Dalawan Leepheng, Piyawan Klamchuen, Annop Phromyothin, Darinee Polymers (Basel) Article Novel molecularly imprinted polymers (MIPs) represent a selectively recognized technique for electrochemical detection design. This rapid and simple method prepared via chemical synthesis consists of a monomer crosslinked with an initiator, whereas low sensitivity remains a drawback. Nanomaterials can improve charge transfer for MIP surface modification in order to overcome this problem. SPIONs have semiconductor and superparamagnetic properties that can enhance carrier mobility, causing high sensitivity of electrochemical detection. In this work, surface modification was achieved with a combination of MIP and SPIONs for gluten detection. The SPIONs were synthesized via the chemical co-precipitation method and mixed with MIPs by polymerizing gluten and methyl methacrylate (MMA), presented as a template and a monomer. Magnetic MIP (MMIP) was modified on a carbon-plate electrode. The morphology of modified electrode surfaces was determined by scanning electron microscopy–energy-dispersive X-ray spectrometry. The performance of the MMIP electrode was confirmed by cyclic voltammetry, amperometry, and electrochemical impedance spectroscopy. The MMIP electrode for gluten detection shows a dynamic linear range of 5–50 ppm, with a correlation coefficient of 0.994 and a low detection limit of 1.50 ppm, which is less than the U.S. Food and Drug Administration requirements (20 ppm); moreover, it exhibits excellent selectivity, sensitivity, stability, and reproducibility. MDPI 2021-12-27 /pmc/articles/PMC8747701/ /pubmed/35012114 http://dx.doi.org/10.3390/polym14010091 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Limthin, Dalawan Leepheng, Piyawan Klamchuen, Annop Phromyothin, Darinee Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles |
title | Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles |
title_full | Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles |
title_fullStr | Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles |
title_full_unstemmed | Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles |
title_short | Enhancement of Electrochemical Detection of Gluten with Surface Modification Based on Molecularly Imprinted Polymers Combined with Superparamagnetic Iron Oxide Nanoparticles |
title_sort | enhancement of electrochemical detection of gluten with surface modification based on molecularly imprinted polymers combined with superparamagnetic iron oxide nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747701/ https://www.ncbi.nlm.nih.gov/pubmed/35012114 http://dx.doi.org/10.3390/polym14010091 |
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