Cargando…

Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(β-amino esters), and Hydroxyapatite

New composite 3D scaffolds were developed as a combination of synthetic polymer, poly(2-hydroxyethyl methacrylate) (PHEMA), and a natural polymer, gelatin, with a ceramic component, nanohydroxyapatite (ID nHAp) dopped with metal ions. The combination of a synthetic polymer, to be able to tune the st...

Descripción completa

Detalles Bibliográficos
Autores principales: Filipović, Vuk V., Babić Radić, Marija M., Vuković, Jovana S., Vukomanović, Marija, Rubert, Marina, Hofmann, Sandra, Müller, Ralph, Tomić, Simonida Lj.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747754/
https://www.ncbi.nlm.nih.gov/pubmed/35012041
http://dx.doi.org/10.3390/polym14010018
_version_ 1784630904702894080
author Filipović, Vuk V.
Babić Radić, Marija M.
Vuković, Jovana S.
Vukomanović, Marija
Rubert, Marina
Hofmann, Sandra
Müller, Ralph
Tomić, Simonida Lj.
author_facet Filipović, Vuk V.
Babić Radić, Marija M.
Vuković, Jovana S.
Vukomanović, Marija
Rubert, Marina
Hofmann, Sandra
Müller, Ralph
Tomić, Simonida Lj.
author_sort Filipović, Vuk V.
collection PubMed
description New composite 3D scaffolds were developed as a combination of synthetic polymer, poly(2-hydroxyethyl methacrylate) (PHEMA), and a natural polymer, gelatin, with a ceramic component, nanohydroxyapatite (ID nHAp) dopped with metal ions. The combination of a synthetic polymer, to be able to tune the structure and the physicochemical and mechanical properties, and a natural polymer, to ensure the specific biological functions of the scaffold, with inorganic filler was applied. The goal was to make a new material with superior properties for applications in the biomedical field which mimics as closely as possible the native bone extracellular matrix (ECM). Biodegradable PHEMA hydrogel was obtained by crosslinking HEMA by poly(β-amino esters) (PBAE). The scaffold’s physicochemical and mechanical properties, in vitro degradation, and biological activity were assessed so to study the effects of the incorporation of nHAp in the (PHEMA/PBAE/gelatin) hydrogel, as well as the effect of the different pore-forming methods. Cryogels had higher elasticity, swelling, porosity, and percent of mass loss during degradation than the samples obtained by porogenation. The composite scaffolds had a higher mechanical strength, 10.14 MPa for the porogenated samples and 5.87 MPa for the cryogels, but a slightly lower degree of swelling, percent of mass loss, and porosity than the hybrid ones. All the scaffolds were nontoxic and had a high cell adhesion rate, which was 15–20% higher in the composite samples. Cell metabolic activity after 2 and 7 days of culture was higher in the composites, although not statistically different. After 28 days, cell metabolic activity was similar in all scaffolds and the TCP control. No effect of integrating nHAp into the scaffolds on osteogenic cell differentiation could be observed. Synergetic effects occurred which influenced the mechanical behavior, structure, physicochemical properties, and interactions with biological species.
format Online
Article
Text
id pubmed-8747754
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87477542022-01-11 Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(β-amino esters), and Hydroxyapatite Filipović, Vuk V. Babić Radić, Marija M. Vuković, Jovana S. Vukomanović, Marija Rubert, Marina Hofmann, Sandra Müller, Ralph Tomić, Simonida Lj. Polymers (Basel) Article New composite 3D scaffolds were developed as a combination of synthetic polymer, poly(2-hydroxyethyl methacrylate) (PHEMA), and a natural polymer, gelatin, with a ceramic component, nanohydroxyapatite (ID nHAp) dopped with metal ions. The combination of a synthetic polymer, to be able to tune the structure and the physicochemical and mechanical properties, and a natural polymer, to ensure the specific biological functions of the scaffold, with inorganic filler was applied. The goal was to make a new material with superior properties for applications in the biomedical field which mimics as closely as possible the native bone extracellular matrix (ECM). Biodegradable PHEMA hydrogel was obtained by crosslinking HEMA by poly(β-amino esters) (PBAE). The scaffold’s physicochemical and mechanical properties, in vitro degradation, and biological activity were assessed so to study the effects of the incorporation of nHAp in the (PHEMA/PBAE/gelatin) hydrogel, as well as the effect of the different pore-forming methods. Cryogels had higher elasticity, swelling, porosity, and percent of mass loss during degradation than the samples obtained by porogenation. The composite scaffolds had a higher mechanical strength, 10.14 MPa for the porogenated samples and 5.87 MPa for the cryogels, but a slightly lower degree of swelling, percent of mass loss, and porosity than the hybrid ones. All the scaffolds were nontoxic and had a high cell adhesion rate, which was 15–20% higher in the composite samples. Cell metabolic activity after 2 and 7 days of culture was higher in the composites, although not statistically different. After 28 days, cell metabolic activity was similar in all scaffolds and the TCP control. No effect of integrating nHAp into the scaffolds on osteogenic cell differentiation could be observed. Synergetic effects occurred which influenced the mechanical behavior, structure, physicochemical properties, and interactions with biological species. MDPI 2021-12-22 /pmc/articles/PMC8747754/ /pubmed/35012041 http://dx.doi.org/10.3390/polym14010018 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Filipović, Vuk V.
Babić Radić, Marija M.
Vuković, Jovana S.
Vukomanović, Marija
Rubert, Marina
Hofmann, Sandra
Müller, Ralph
Tomić, Simonida Lj.
Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(β-amino esters), and Hydroxyapatite
title Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(β-amino esters), and Hydroxyapatite
title_full Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(β-amino esters), and Hydroxyapatite
title_fullStr Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(β-amino esters), and Hydroxyapatite
title_full_unstemmed Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(β-amino esters), and Hydroxyapatite
title_short Biodegradable Hydrogel Scaffolds Based on 2-Hydroxyethyl Methacrylate, Gelatin, Poly(β-amino esters), and Hydroxyapatite
title_sort biodegradable hydrogel scaffolds based on 2-hydroxyethyl methacrylate, gelatin, poly(β-amino esters), and hydroxyapatite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8747754/
https://www.ncbi.nlm.nih.gov/pubmed/35012041
http://dx.doi.org/10.3390/polym14010018
work_keys_str_mv AT filipovicvukv biodegradablehydrogelscaffoldsbasedon2hydroxyethylmethacrylategelatinpolybaminoestersandhydroxyapatite
AT babicradicmarijam biodegradablehydrogelscaffoldsbasedon2hydroxyethylmethacrylategelatinpolybaminoestersandhydroxyapatite
AT vukovicjovanas biodegradablehydrogelscaffoldsbasedon2hydroxyethylmethacrylategelatinpolybaminoestersandhydroxyapatite
AT vukomanovicmarija biodegradablehydrogelscaffoldsbasedon2hydroxyethylmethacrylategelatinpolybaminoestersandhydroxyapatite
AT rubertmarina biodegradablehydrogelscaffoldsbasedon2hydroxyethylmethacrylategelatinpolybaminoestersandhydroxyapatite
AT hofmannsandra biodegradablehydrogelscaffoldsbasedon2hydroxyethylmethacrylategelatinpolybaminoestersandhydroxyapatite
AT mullerralph biodegradablehydrogelscaffoldsbasedon2hydroxyethylmethacrylategelatinpolybaminoestersandhydroxyapatite
AT tomicsimonidalj biodegradablehydrogelscaffoldsbasedon2hydroxyethylmethacrylategelatinpolybaminoestersandhydroxyapatite