Engineered HaloTag variants for fluorescence lifetime multiplexing

Self-labeling protein tags such as HaloTag are powerful tools that can label fusion proteins with synthetic fluorophores for use in fluorescence microscopy. Here we introduce HaloTag variants with either increased or decreased brightness and fluorescence lifetime compared with HaloTag7 when labeled...

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Detalles Bibliográficos
Autores principales: Frei, Michelle S., Tarnawski, Miroslaw, Roberti, M. Julia, Koch, Birgit, Hiblot, Julien, Johnsson, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748199/
https://www.ncbi.nlm.nih.gov/pubmed/34916672
http://dx.doi.org/10.1038/s41592-021-01341-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748199/
https://www.ncbi.nlm.nih.gov/pubmed/34916672
http://dx.doi.org/10.1038/s41592-021-01341-x

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