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Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies

Blood feeding and host-seeking behaviors of a mosquito play an imperative role in determining its vectorial capacity in transmitting pathogens. Unfortunately, limited information is available regarding blood feeding behavior of Anopheles species in Malaysia. Collection of resting Anopheles mosquitoe...

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Autores principales: Jeyaprakasam, Nantha Kumar, Low, Van Lun, Liew, Jonathan Wee Kent, Pramasivan, Sandthya, Wan-Sulaiman, Wan-Yusoff, Saeung, Atiporn, Vythilingam, Indra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748441/
https://www.ncbi.nlm.nih.gov/pubmed/35013403
http://dx.doi.org/10.1038/s41598-021-04106-w
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author Jeyaprakasam, Nantha Kumar
Low, Van Lun
Liew, Jonathan Wee Kent
Pramasivan, Sandthya
Wan-Sulaiman, Wan-Yusoff
Saeung, Atiporn
Vythilingam, Indra
author_facet Jeyaprakasam, Nantha Kumar
Low, Van Lun
Liew, Jonathan Wee Kent
Pramasivan, Sandthya
Wan-Sulaiman, Wan-Yusoff
Saeung, Atiporn
Vythilingam, Indra
author_sort Jeyaprakasam, Nantha Kumar
collection PubMed
description Blood feeding and host-seeking behaviors of a mosquito play an imperative role in determining its vectorial capacity in transmitting pathogens. Unfortunately, limited information is available regarding blood feeding behavior of Anopheles species in Malaysia. Collection of resting Anopheles mosquitoes for blood meal analysis poses a great challenge especially for forest dwelling mosquitoes. Therefore, a laboratory-based study was conducted to evaluate the potential use of mosquitoes caught using human landing catch (HLC) for blood meal analysis, and subsequently to document blood feeding behavior of local Anopheles mosquitoes in Peninsular Malaysia. The laboratory-based experiment from this study revealed that mosquitoes caught using HLC had the potential to be used for blood meal analysis. Besides HLC, mosquitoes were also collected using manual aspirator and Mosquito Magnet. Overall, 47.4% of 321 field-caught Anopheles mosquitoes belonging to six species were positive for vertebrate host DNA in their blood meal. The most frequent blood meal source was human (45.9%) followed by wild boar (27.4%), dog (15.3%) and monkey (7.5%). Interestingly, only Anopheles cracens and Anopheles introlatus (Leucosphyrus Group) fed on monkey. This study further confirmed that members of the Leucosphyrus Group are the predominant vectors for knowlesi malaria transmission in Peninsular Malaysia mainly due to their simio-anthropophagic feeding behavior.
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spelling pubmed-87484412022-01-11 Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies Jeyaprakasam, Nantha Kumar Low, Van Lun Liew, Jonathan Wee Kent Pramasivan, Sandthya Wan-Sulaiman, Wan-Yusoff Saeung, Atiporn Vythilingam, Indra Sci Rep Article Blood feeding and host-seeking behaviors of a mosquito play an imperative role in determining its vectorial capacity in transmitting pathogens. Unfortunately, limited information is available regarding blood feeding behavior of Anopheles species in Malaysia. Collection of resting Anopheles mosquitoes for blood meal analysis poses a great challenge especially for forest dwelling mosquitoes. Therefore, a laboratory-based study was conducted to evaluate the potential use of mosquitoes caught using human landing catch (HLC) for blood meal analysis, and subsequently to document blood feeding behavior of local Anopheles mosquitoes in Peninsular Malaysia. The laboratory-based experiment from this study revealed that mosquitoes caught using HLC had the potential to be used for blood meal analysis. Besides HLC, mosquitoes were also collected using manual aspirator and Mosquito Magnet. Overall, 47.4% of 321 field-caught Anopheles mosquitoes belonging to six species were positive for vertebrate host DNA in their blood meal. The most frequent blood meal source was human (45.9%) followed by wild boar (27.4%), dog (15.3%) and monkey (7.5%). Interestingly, only Anopheles cracens and Anopheles introlatus (Leucosphyrus Group) fed on monkey. This study further confirmed that members of the Leucosphyrus Group are the predominant vectors for knowlesi malaria transmission in Peninsular Malaysia mainly due to their simio-anthropophagic feeding behavior. Nature Publishing Group UK 2022-01-10 /pmc/articles/PMC8748441/ /pubmed/35013403 http://dx.doi.org/10.1038/s41598-021-04106-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jeyaprakasam, Nantha Kumar
Low, Van Lun
Liew, Jonathan Wee Kent
Pramasivan, Sandthya
Wan-Sulaiman, Wan-Yusoff
Saeung, Atiporn
Vythilingam, Indra
Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies
title Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies
title_full Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies
title_fullStr Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies
title_full_unstemmed Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies
title_short Blood meal analysis of Anopheles vectors of simian malaria based on laboratory and field studies
title_sort blood meal analysis of anopheles vectors of simian malaria based on laboratory and field studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748441/
https://www.ncbi.nlm.nih.gov/pubmed/35013403
http://dx.doi.org/10.1038/s41598-021-04106-w
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