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Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives

The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives....

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Autores principales: Kostyuk, Alexander I., Tossounian, Maria-Armineh, Panova, Anastasiya S., Thauvin, Marion, Raevskii, Roman I., Ezeriņa, Daria, Wahni, Khadija, Van Molle, Inge, Sergeeva, Anastasia D., Vertommen, Didier, Gorokhovatsky, Andrey Yu., Baranov, Mikhail S., Vriz, Sophie, Messens, Joris, Bilan, Dmitry S., Belousov, Vsevolod V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748444/
https://www.ncbi.nlm.nih.gov/pubmed/35013284
http://dx.doi.org/10.1038/s41467-021-27796-2
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author Kostyuk, Alexander I.
Tossounian, Maria-Armineh
Panova, Anastasiya S.
Thauvin, Marion
Raevskii, Roman I.
Ezeriņa, Daria
Wahni, Khadija
Van Molle, Inge
Sergeeva, Anastasia D.
Vertommen, Didier
Gorokhovatsky, Andrey Yu.
Baranov, Mikhail S.
Vriz, Sophie
Messens, Joris
Bilan, Dmitry S.
Belousov, Vsevolod V.
author_facet Kostyuk, Alexander I.
Tossounian, Maria-Armineh
Panova, Anastasiya S.
Thauvin, Marion
Raevskii, Roman I.
Ezeriņa, Daria
Wahni, Khadija
Van Molle, Inge
Sergeeva, Anastasia D.
Vertommen, Didier
Gorokhovatsky, Andrey Yu.
Baranov, Mikhail S.
Vriz, Sophie
Messens, Joris
Bilan, Dmitry S.
Belousov, Vsevolod V.
author_sort Kostyuk, Alexander I.
collection PubMed
description The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 10(6) M(−1)s(−1) range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model.
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spelling pubmed-87484442022-01-20 Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives Kostyuk, Alexander I. Tossounian, Maria-Armineh Panova, Anastasiya S. Thauvin, Marion Raevskii, Roman I. Ezeriņa, Daria Wahni, Khadija Van Molle, Inge Sergeeva, Anastasia D. Vertommen, Didier Gorokhovatsky, Andrey Yu. Baranov, Mikhail S. Vriz, Sophie Messens, Joris Bilan, Dmitry S. Belousov, Vsevolod V. Nat Commun Article The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 10(6) M(−1)s(−1) range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model. Nature Publishing Group UK 2022-01-10 /pmc/articles/PMC8748444/ /pubmed/35013284 http://dx.doi.org/10.1038/s41467-021-27796-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kostyuk, Alexander I.
Tossounian, Maria-Armineh
Panova, Anastasiya S.
Thauvin, Marion
Raevskii, Roman I.
Ezeriņa, Daria
Wahni, Khadija
Van Molle, Inge
Sergeeva, Anastasia D.
Vertommen, Didier
Gorokhovatsky, Andrey Yu.
Baranov, Mikhail S.
Vriz, Sophie
Messens, Joris
Bilan, Dmitry S.
Belousov, Vsevolod V.
Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
title Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
title_full Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
title_fullStr Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
title_full_unstemmed Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
title_short Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
title_sort hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748444/
https://www.ncbi.nlm.nih.gov/pubmed/35013284
http://dx.doi.org/10.1038/s41467-021-27796-2
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