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Increased expression of the fluorescent reporter protein ymNeonGreen in Saccharomyces cerevisiae by reducing RNA secondary structure near the start codon.

Expression of a new fluorescent reporter protein called mNeonGreen, that is not based on the jellyfish green fluorescent protein (GFP) sequence, shows increased brightness and folding speed compared to enhanced GFP. However, in vivo brightness of mNeonGreen and its yeast-optimized variant ymNeonGree...

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Detalles Bibliográficos
Autores principales: Hector, Ronald E., Mertens, Jeffrey A., Nichols, Nancy N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749125/
https://www.ncbi.nlm.nih.gov/pubmed/35036336
http://dx.doi.org/10.1016/j.btre.2021.e00697