Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay

Mobilized colistin resistance (mcr) genes confer resistance to colistin, a last-resort antibiotic for multidrug-resistant Gram-negative infections. In this case report, we describe a novel lipid-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) diagnos...

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Detalles Bibliográficos
Autores principales: Smith, Richard D., Izac, Jerilyn R., Ha, Michael, Yang, Hyojik, Johnson, J. Kristie, Ernst, Robert K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Case Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749148/
https://www.ncbi.nlm.nih.gov/pubmed/35024564
http://dx.doi.org/10.1099/acmi.0.000309
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author Smith, Richard D.
Izac, Jerilyn R.
Ha, Michael
Yang, Hyojik
Johnson, J. Kristie
Ernst, Robert K.
author_facet Smith, Richard D.
Izac, Jerilyn R.
Ha, Michael
Yang, Hyojik
Johnson, J. Kristie
Ernst, Robert K.
author_sort Smith, Richard D.
collection PubMed
description Mobilized colistin resistance (mcr) genes confer resistance to colistin, a last-resort antibiotic for multidrug-resistant Gram-negative infections. In this case report, we describe a novel lipid-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) diagnostic used to rapidly identify an mcr-1-positive Escherichia coli directly from a patient with a urinary tract infection without the need for ex vivo growth.
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spelling pubmed-87491482022-01-11 Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay Smith, Richard D. Izac, Jerilyn R. Ha, Michael Yang, Hyojik Johnson, J. Kristie Ernst, Robert K. Access Microbiol Case Reports Mobilized colistin resistance (mcr) genes confer resistance to colistin, a last-resort antibiotic for multidrug-resistant Gram-negative infections. In this case report, we describe a novel lipid-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) diagnostic used to rapidly identify an mcr-1-positive Escherichia coli directly from a patient with a urinary tract infection without the need for ex vivo growth. Microbiology Society 2021-12-17 /pmc/articles/PMC8749148/ /pubmed/35024564 http://dx.doi.org/10.1099/acmi.0.000309 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. The Microbiology Society waived the open access fees for this article.
spellingShingle Case Reports
Smith, Richard D.
Izac, Jerilyn R.
Ha, Michael
Yang, Hyojik
Johnson, J. Kristie
Ernst, Robert K.
Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay
title Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay
title_full Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay
title_fullStr Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay
title_full_unstemmed Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay
title_short Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay
title_sort rapid identification of mcr-1-positive escherichia coli from patient urine using a novel lipid-based maldi-tof-ms assay
topic Case Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749148/
https://www.ncbi.nlm.nih.gov/pubmed/35024564
http://dx.doi.org/10.1099/acmi.0.000309
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