Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages

Upon viral infection, several proteins in the innate signaling pathway form aggregates, which in turn promote the activation of innate antiviral immune response. In this protocol, we use herpes simplex virus type 1 (HSV-1) to infect mouse peritoneal macrophages, and show how to detect the aggregatio...

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Detalles Bibliográficos
Autores principales: Yan, Zhenzhen, Liu, Hansen, Gao, Chengjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749298/
https://www.ncbi.nlm.nih.gov/pubmed/35036960
http://dx.doi.org/10.1016/j.xpro.2021.101080
Descripción
Sumario:Upon viral infection, several proteins in the innate signaling pathway form aggregates, which in turn promote the activation of innate antiviral immune response. In this protocol, we use herpes simplex virus type 1 (HSV-1) to infect mouse peritoneal macrophages, and show how to detect the aggregation of TBK1 upon viral infection. The protocol is adaptable for other proteins and other viruses. For complete details on the use and execution of this profile, please refer to Yan et al. (2021).

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