Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages

Upon viral infection, several proteins in the innate signaling pathway form aggregates, which in turn promote the activation of innate antiviral immune response. In this protocol, we use herpes simplex virus type 1 (HSV-1) to infect mouse peritoneal macrophages, and show how to detect the aggregatio...

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Detalles Bibliográficos
Autores principales: Yan, Zhenzhen, Liu, Hansen, Gao, Chengjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749298/
https://www.ncbi.nlm.nih.gov/pubmed/35036960
http://dx.doi.org/10.1016/j.xpro.2021.101080
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author Yan, Zhenzhen
Liu, Hansen
Gao, Chengjiang
author_facet Yan, Zhenzhen
Liu, Hansen
Gao, Chengjiang
author_sort Yan, Zhenzhen
collection PubMed
description Upon viral infection, several proteins in the innate signaling pathway form aggregates, which in turn promote the activation of innate antiviral immune response. In this protocol, we use herpes simplex virus type 1 (HSV-1) to infect mouse peritoneal macrophages, and show how to detect the aggregation of TBK1 upon viral infection. The protocol is adaptable for other proteins and other viruses. For complete details on the use and execution of this profile, please refer to Yan et al. (2021).
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spelling pubmed-87492982022-01-13 Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages Yan, Zhenzhen Liu, Hansen Gao, Chengjiang STAR Protoc Protocol Upon viral infection, several proteins in the innate signaling pathway form aggregates, which in turn promote the activation of innate antiviral immune response. In this protocol, we use herpes simplex virus type 1 (HSV-1) to infect mouse peritoneal macrophages, and show how to detect the aggregation of TBK1 upon viral infection. The protocol is adaptable for other proteins and other viruses. For complete details on the use and execution of this profile, please refer to Yan et al. (2021). Elsevier 2022-01-08 /pmc/articles/PMC8749298/ /pubmed/35036960 http://dx.doi.org/10.1016/j.xpro.2021.101080 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yan, Zhenzhen
Liu, Hansen
Gao, Chengjiang
Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages
title Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages
title_full Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages
title_fullStr Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages
title_full_unstemmed Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages
title_short Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages
title_sort native-page analysis of protein aggregation upon viral infection in mouse macrophages
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749298/
https://www.ncbi.nlm.nih.gov/pubmed/35036960
http://dx.doi.org/10.1016/j.xpro.2021.101080
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