Antimicrobial Resistance and PFGE Molecular Typing of Salmonella enterica serovar Gallinarum Isolates from Chickens in South Korea from 2013 to 2018

SIMPLE SUMMARY: Salmonella enterica serovar Gallinarum (S. enterica ser. Gallinarum) is a host-specific agent of fowl typhoid (FT). This is one of the most important bacterial infections in the poultry industry in both developing and developed countries, including South Korea. The use of antimicrobial drugs is the first choice for disease control. Antimicrobials, such as β-lactams, aminoglycosides, and fluoroquinolones, are frequently used to treat FT. However, the continuous use of antimicrobial drugs has led to the emergence and persistence of antimicrobial-resistant S. enterica ser. Gallinarum. In this study, we analyzed the antimicrobial susceptibility and epidemiological relationship of thirty isolates of S. enterica ser. Gallinarum isolated from poultry farms with an FT outbreak from 2013 to 2018 in South Korea. All the isolates showed a multi-drug resistant (MDR) phenotype. This study confirmed horizontal transmission and cross-contamination between farms within the same integrated poultry company or between farms belonging to different companies. The characterization of these isolates would be helpful to develop prevention and control strategies for the MDR S. enterica ser. Gallinarum infection in South Korea. ABSTRACT: Antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) genotypes of collected S. enterica ser. Gallinarum isolates were investigated to examine the epidemiological relationship between field outbreak isolates of S. enterica ser. Gallinarum. Thirty S. enterica ser. Gallinarum isolates collected from poultry farms with FT outbreaks from 2013 to 2018 in South Korea were analyzed. All isolates were resistant to at least 3 of the 18 antimicrobials tested and exhibited an MDR phenotype. All isolates showed resistance to streptomycin, sulfisoxazole, and colistin. One isolate was resistant to 9 antimicrobials. The antimicrobial resistance profile, streptomycin-sulfisoxazole-colistin-nalidixic acid-ciprofloxacin-gentamicin (18/30, 60.0%), was the most prevalent. PFGE types were classified into 10 groups with a 100% correlation cutoff in dendrograms for 30 field isolates. The dominant PFGE types were 1 (8/30, 26.7%), 4 (7/30, 23.3%), and 9 (5/30, 16.7%). Interestingly some isolates collected from the same and different companies had the same PFGE type. We reported a high MDR rate in S. enterica ser. Gallinarum isolates. The present study highlights the occurrence of horizontal spread and cyclic contamination of MDR S. enterica ser. Gallinarum within the same company. Furthermore, we showed cross-contamination between different companies. The characterization of these isolates would be helpful in the development of prevention and control strategies for MDR S. enterica ser. Gallinarum infection in South Korea.