Canine and Feline Testicular Preservation

SIMPLE SUMMARY: Testicular cryopreservation enables the maintenance of reproductive potential, the creation of germplasm banks and the transport of genetic material between different regions. This biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have already died or that need to undergo gonadotoxic treatments. Despite advances in the use of cryopreserved testicular fragments, protocols that can be used in the clinical routine of dogs and cats have not yet been established. Due to the great importance of the topic, the objective of this review is to provide an overview of the subject, approaching the main works on testicular cryopreservation in dogs and cats. ABSTRACT: The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats.