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Advances in Design Strategies of Multiplex Electrochemical Aptasensors
In recent years, the need for simple, fast, and economical detection of food and environmental contaminants, and the necessity to monitor biomarkers of different diseases have considerably accelerated the development of biosensor technology. However, designing biosensors capable of simultaneous dete...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749765/ https://www.ncbi.nlm.nih.gov/pubmed/35009703 http://dx.doi.org/10.3390/s22010161 |
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author | Grabowska, Iwona Hepel, Maria Kurzątkowska-Adaszyńska, Katarzyna |
author_facet | Grabowska, Iwona Hepel, Maria Kurzątkowska-Adaszyńska, Katarzyna |
author_sort | Grabowska, Iwona |
collection | PubMed |
description | In recent years, the need for simple, fast, and economical detection of food and environmental contaminants, and the necessity to monitor biomarkers of different diseases have considerably accelerated the development of biosensor technology. However, designing biosensors capable of simultaneous determination of two or more analytes in a single measurement, for example on a single working electrode in single solution, is still a great challenge. On the other hand, such analysis offers many advantages compared to single analyte tests, such as cost per test, labor, throughput, and convenience. Because of the high sensitivity and scalability of the electrochemical detection systems on the one hand and the specificity of aptamers on the other, the electrochemical aptasensors are considered to be highly effective devices for simultaneous detection of multiple-target analytes. In this review, we describe and evaluate multi-label approaches based on (1) metal quantum dots and metal ions, (2) redox labels, and (3) enzyme labels. We focus on recently developed strategies for multiplex sensing using electrochemical aptasensors. Furthermore, we emphasize the use of different nanomaterials in the construction of these aptasensors. Based on examples from the existing literature, we highlight recent applications of multiplexed detection platforms in clinical diagnostics, food control, and environmental monitoring. Finally, we discuss the advantages and disadvantages of the aptasensors developed so far, and debate possible challenges and prospects. |
format | Online Article Text |
id | pubmed-8749765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87497652022-01-12 Advances in Design Strategies of Multiplex Electrochemical Aptasensors Grabowska, Iwona Hepel, Maria Kurzątkowska-Adaszyńska, Katarzyna Sensors (Basel) Review In recent years, the need for simple, fast, and economical detection of food and environmental contaminants, and the necessity to monitor biomarkers of different diseases have considerably accelerated the development of biosensor technology. However, designing biosensors capable of simultaneous determination of two or more analytes in a single measurement, for example on a single working electrode in single solution, is still a great challenge. On the other hand, such analysis offers many advantages compared to single analyte tests, such as cost per test, labor, throughput, and convenience. Because of the high sensitivity and scalability of the electrochemical detection systems on the one hand and the specificity of aptamers on the other, the electrochemical aptasensors are considered to be highly effective devices for simultaneous detection of multiple-target analytes. In this review, we describe and evaluate multi-label approaches based on (1) metal quantum dots and metal ions, (2) redox labels, and (3) enzyme labels. We focus on recently developed strategies for multiplex sensing using electrochemical aptasensors. Furthermore, we emphasize the use of different nanomaterials in the construction of these aptasensors. Based on examples from the existing literature, we highlight recent applications of multiplexed detection platforms in clinical diagnostics, food control, and environmental monitoring. Finally, we discuss the advantages and disadvantages of the aptasensors developed so far, and debate possible challenges and prospects. MDPI 2021-12-27 /pmc/articles/PMC8749765/ /pubmed/35009703 http://dx.doi.org/10.3390/s22010161 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Grabowska, Iwona Hepel, Maria Kurzątkowska-Adaszyńska, Katarzyna Advances in Design Strategies of Multiplex Electrochemical Aptasensors |
title | Advances in Design Strategies of Multiplex Electrochemical Aptasensors |
title_full | Advances in Design Strategies of Multiplex Electrochemical Aptasensors |
title_fullStr | Advances in Design Strategies of Multiplex Electrochemical Aptasensors |
title_full_unstemmed | Advances in Design Strategies of Multiplex Electrochemical Aptasensors |
title_short | Advances in Design Strategies of Multiplex Electrochemical Aptasensors |
title_sort | advances in design strategies of multiplex electrochemical aptasensors |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749765/ https://www.ncbi.nlm.nih.gov/pubmed/35009703 http://dx.doi.org/10.3390/s22010161 |
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